The DOI matches its Crossref record.
Improved procedures for immunoferritin labeling of ultrathin frozen sections.
Open accessAbstract
In employing fixed frozen ultrathin sections as substrates for immunoferritin labeling of intracellular antigens, we have found that conventional glutaraldehyde fixation sometimes permits very little specific staining of the sections, either because it inactivates certain protein antigens, or because it renders them inaccessible to the antibody stains. We have developed several fixation procedures that are chemically milder and allow a uniform but less extensive cross-linking of the specimen. With these procedures and precautions in the handling of the more fragile frozen sections, excellent structural preservation and specific immunoferritin labeling has been achieved with several systems.
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Citations
Cited by
Louvard, D. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.7.4132 · Cited by 331
Identification of human endogenous retroviruses with complex mRNA expression and particle formation.
Löwer, R., Boller, K., Hasenmaier, B., Korbmacher, C., Müller-Lantzsch, N., Löwer, J., & Kurth, R. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.10.4480 · Cited by 268
Metadata
- Authors
- Tokuyasu, K. T., & Singer, S. J.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1976-12-01
- Volume
- 71
- Issue
- 3
- Pages
- 894-906
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2095227193
- Cited by
- 277
- References
- 0
- FWCI
- 9.37
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Improved procedures for immunoferritin labeling of ultrathin frozen sections. https://app.cochat.ai/papers/10.1083/jcb.71.3.894
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- Tokuyasu, K. T., & Singer, S. J. (1976). Improved procedures for immunoferritin labeling of ultrathin frozen sections. The Journal of cell biology. https://doi.org/10.1083/jcb.71.3.894
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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