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Cited by 161Bone Metabolism and DiseasesBone health and osteoporosis researchAlkaline Phosphatase Research Studies

ELECTRON MICROSCOPY OF OSTEOCLASTS IN HEALING FRACTURES OF RAT BONE

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Gonzales, Federico, Karnovsky, Morris J.·1961·The Journal of Cell Biology
DOI 10.1083/jcb.9.2.299PMC PMC2225009
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Abstract

Osmium-fixed, undecalcified, callus tissue from healing fractures of rat tibias was sectioned with a diamond knife for study with the electron microscope. Large multinucleated cells were found adjacent to bone. A characteristic labyrinthine infolded border was consistently seen in parts of the cells close to the bone surface. The innermost parts of this "ruffled border" gave rise to vacuoles. The bone surface was always disrupted under the "ruffled border" of the cells. Needle-like crystals were seen at the osseous fringe, within folds in the ruffled border as well as within vacuoles deeper in the cells. Collagen fibers denuded of crystals were never observed. Mitochondria, containing clusters of fine granules, were abundant. The part of the cell away from bone contained rough endoplasmic reticulum and the cell membrane was thrown into irregular microvilli. These observations are discussed in relation to current concepts of osteoclastic resorption of bone.

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  • Ca- and P-containing granules were identified within the mitochondria of osteoclasts, chondrocytes, osteoblasts, osteocytes, calcifying cartilage, and mineralizing bone if samples were prepared with anhydrous or cryo techniques.

    “Ca- and P-containing granules were identified within the mitochondria of osteoclasts [116], chondrocytes [117, 118], osteoblasts [119], osteocytes [120–122], calcifying cartilage [123], and mineralizing bone [124–126] if samples were prepared with anhydrous or cryo techniques.”

    From A Review of Phosphate Mineral Nucleation in Biology and Geobiology (2013) · cites this paper as [116]

    The figure captions (pp. 313–314) identify granules within osteoclast mitochondria. But the Discussion says their interpretation awaits further work; it does not identify them as calcium- and phosphorus-containing. Materials and Methods (p. 300) describes buffered osmium fixation followed by ethanol dehydration, not anhydrous or cryo preparation. The other cell and tissue types have separate citations and are not attributed to this source. (LLM, conf=0.91)

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Authors
Gonzales, F., & Karnovsky, M. J.
Affiliations
Harvard University (US)
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
1961-02-01
Volume
9
Issue
2
Pages
299-316
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.9.2.299
PMC
PMC2225009
OpenAlex
W2065456574
Cited by
161
References
12
Citations by year
2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 5 · 2014: 1 · 2013: 2
FWCI
3.66
Type
journal article
Language
en
Open access
Locations
  • The Journal of Cell Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Alkaline Phosphatase Research Studies
Keywords
osteoclasts, bone regeneration, callus, ruffled border, bone resorption, multinucleated cells, vacuoles, needle-like crystals, mitochondria, rough endoplasmic reticulum, microvilli, tibia, rats
MeSH terms
Animals, Bone and Bones, Bone Resorption, Cell Membrane, Microscopy, Electron, Mitochondria, Osteoclasts, Vacuoles, Wound Healing, Fractures, Bone, Rats

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