The DOI matches its Crossref record.
Synthesis and secretion of thrombospondin by cultured human endothelial cells.
Open accessAbstract
Thrombospondin, the major glycoprotein released from alpha-granules of thrombin-stimulated platelets, is a disulfide-bonded trimer of 160 kilodalton subunits and apparently functions as a platelet lectin. Because cultured human umbilical vein endothelial cells synthesize and secrete a glycoprotein (GP-160) which is a disulfide-bonded multimer of 160 kdalton subunits, the possibility that GP-160 is thrombospondin was investigated. Tritiated GP-160 could be immunoisolated from [3H]leucine-labeled endothelial cell postculture medium using a rabbit antiserum to human platelet thrombospondin. Thrombospondin and GP-160 comigrated in two different two-dimensional electrophoretic systems. Both proteins are disulfide-bonded trimers of acidic 160-kdalton subunits. A competitive radioimmunoassay for binding of 125I-thrombospondin to the rabbit antibodies indicated that 49 micrograms of thrombospondin antigen per 10(6) confluent endothelial cells accumulated in postculture medium over 24 h. Thus, endothelial cells secrete large amounts of a glycoprotein that is identical or very similar to platelet thrombospondin.
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Cited by
Lillis, A. P., Van Duyn, L. B., Murphy-Ullrich, J. E., & Strickland, D. K. (2008) · Physiological Reviews · doi:10.1152/physrev.00033.2007 · Cited by 669
Isolation of the thrombospondin membrane receptor.
Asch, A. S., Barnwell, J., Silverstein, R. L., & Nachman, R. L. (1987) · Journal of Clinical Investigation · doi:10.1172/jci112918 · Cited by 508
van Mourik, J. A., Lawrence, D. A., & Loskutoff, D. J. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)42691-3 · Cited by 450
Taraboletti, G., Roberts, D., Liotta, L. A., & Giavazzi, R. (1990) · The Journal of cell biology · doi:10.1083/jcb.111.2.765 · Cited by 411
Barnwell, J. W., Asch, A. S., Nachman, R. L., Yamaya, M., Aikawa, M., & Ingravallo, P. (1989) · Journal of Clinical Investigation · doi:10.1172/jci114234 · Cited by 373
Metadata
- Authors
- Mosher, D. F., Doyle, M. J., & Jaffe, E. A.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1982-05-01
- Volume
- 93
- Issue
- 2
- Pages
- 343-348
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2085931182
- Cited by
- 304
- References
- 0
- FWCI
- 15.22
- Type
- journal article
- Language
- en
- Open access
- green
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- Mosher, D. F., Doyle, M. J., & Jaffe, E. A. (1982). Synthesis and secretion of thrombospondin by cultured human endothelial cells. The Journal of cell biology. https://doi.org/10.1083/jcb.93.2.343
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