The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Endosome pH measured in single cells by dual fluorescence flow cytometry: rapid acidification of insulin to pH 6.
Open accessAbstract
The acidification of various ligands was measured on a cell by cell basis for cell suspensions by correlated dual fluorescence flow cytometry. Mouse 3T3 cells were incubated with a mixture of fluorescein- and rhodamine-conjugated ligands, and the ratio of fluorescein and rhodamine fluorescence was used as a measure of endosome pH. The calibration of this ratio by both fluorometry and flow cytometry is described. Dual parameter histograms of average endosome pH per cell versus amount of internalization were calculated from this data, for samples in the absence and presence of chloroquine added to neutralize acidic cellular vesicles. The kinetics of acidification of insulin were measured and compared with previous results obtained with the chloroquine ratio technique. Rapid acidification of internalized ligand was observed both for insulin, which was mostly internalized via nonspecific pathways, and for alpha 2-macroglobulin, which was mainly internalized by specific receptor-mediated endocytosis. The average pH observed for internalized insulin was less than pH 6 within 10 min after addition of insulin. At 30 min, the average pH began to decrease to approximately pH 5, presumably because of fusion of endosomes with lysosomes.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Ohkuma, S., & Poole, B. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.7.3327 · Cited by 2,174
Thomas, J. A., Buchsbaum, R. N., Zimniak, A., & Racker, E. (1979) · Biochemistry · doi:10.1021/bi00578a012 · Cited by 2,132
Endocytosis and the recycling of plasma membrane.
Steinman, R. M., Mellman, I. S., Muller, W. A., & Cohn, Z. A. (1983) · The Journal of cell biology · doi:10.1083/jcb.96.1.1 · Cited by 1,450
On the entry of semliki forest virus into BHK-21 cells
Helenius, A., Kartenbeck, J., Simons, K., & Fries, E. (1980) · The Journal of Cell Biology · doi:10.1083/jcb.84.2.404 · Cited by 807
Gonzalez-Noriega, A., Grubb, J. H., Talkad, V, & Sly, W. S. (1980) · The Journal of cell biology · doi:10.1083/jcb.85.3.839 · Cited by 736
Infectious entry pathway of influenza virus in a canine kidney cell line.
Matlin, K. S., Reggio, H., Helenius, A., & Simons, K. (1981) · The Journal of cell biology · doi:10.1083/jcb.91.3.601 · Cited by 694
Rapid acidification of endocytic vesicles containing α2-macroglobulin
Tycko, B., & Maxfield, F. R. (1982) · Cell · doi:10.1016/0092-8674(82)90219-7 · Cited by 671
van Renswoude, J., Bridges, K. R., Harford, J. B., & Klausner, R. D. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.20.6186 · Cited by 488
Diphtheria toxin entry into cells is facilitated by low pH.
Sandvig, K., & Olsnes, S. (1980) · The Journal of cell biology · doi:10.1083/jcb.87.3.828 · Cited by 461
The respiratory burst of phagocytic cells is associated with a rise in vacuolar pH
Segal, A. W., Geisow, M., Garcia, R., Harper, A., & Miller, R. (1981) · Nature · doi:10.1038/290406a0 · Cited by 423
Cited by
Ge, Z., & Liu, S. (2013) · Chemical Society Reviews · doi:10.1039/c3cs60048c · Cited by 914
Qiu, A., Jansen, M., Sakaris, A., Min, S. H., Chattopadhyay, S., Tsai, E., Sandoval, C., Zhao, R., Akabas, M. H., & Goldman, I. D. (2006) · Cell · doi:10.1016/j.cell.2006.09.041 · Cited by 847
A Review on the Synthesis and Functionalization of Gold Nanoparticles as a Drug Delivery Vehicle
Amina, S. J., & Guo, B. (2020) · International Journal of Nanomedicine · doi:10.2147/ijn.s279094 · Cited by 561
The cell biology of systemic insulin function
Tokarz, V. L., MacDonald, P. E., & Klip, A. (2018) · Journal of Cell Biology · doi:10.1083/jcb.201802095 · Cited by 471
“As endosomes acidify, any remaining insulin or partially degraded insulin that escaped complete degradation by IDE dissociates from the IR (Murphy et al., 1984; Fig.”
BackgroundStimuli-responsive nanomaterials for therapeutic protein delivery
Lu, Y., Sun, W., & Gu, Z. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.08.015 · Cited by 415
Metadata
- Authors
- Murphy, R. F., Powers, S., & Cantor, C. R.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1984-05-01
- Volume
- 98
- Issue
- 5
- Pages
- 1757-1762
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2114001610
- Cited by
- 285
- References
- 23
- FWCI
- 4.58
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Endosome pH measured in single cells by dual fluorescence flow cytometry: rapid acidification of insulin to pH 6. https://app.cochat.ai/papers/10.1083/jcb.98.5.1757
- The paper
- Murphy, R. F., Powers, S., & Cantor, C. R. (1984). Endosome pH measured in single cells by dual fluorescence flow cytometry: rapid acidification of insulin to pH 6. The Journal of cell biology. https://doi.org/10.1083/jcb.98.5.1757
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
