The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Soluble cytokine receptors are present in normal human urine.
Open accessAbstract
Affinity chromatography of crude human urinary proteins on either human rIL-6, human rIFN-gamma, or anti-IFN-gamma-R mAb yielded the two respective soluble receptors in significant quantities. A single sequence of 30 amino acid residues was obtained by NH2-terminal microsequencing of the protein peak purified in tandem by affinity chromatography on an IL-6 column and reversed-phase HPLC. This sequence was identical to the predicted NH2-terminal sequence of IL-6-R as previously reported. Analysis of the eluted proteins from both IFN-gamma and anti-IFN-gamma-R columns by inhibition of solid phase RIA, ELISA, SDS-PAGE, and Western blotting proved the existence of soluble IFN-gamma-R in normal urine. Our finding, together with the already known presence of urinary TNF binding proteins and a soluble IL-2-R both in plasma and in urine, indicates that release of soluble cytokine receptors into body fluids is a general phenomenon that occurs under normal physiological conditions.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,221
A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels
Oakley, B. R., Kirsch, D. R., & Morris, N. R. (1980) · Analytical Biochemistry · doi:10.1016/0003-2697(80)90470-4 · Cited by 3,247
Soluble interleukin 2 receptors are released from activated human lymphoid cells in vitro.
Rubin, L. A., Kurman, C. C., Fritz, M. E., Biddison, W. E., Boutin, B., Yarchoan, R., & Nelson, D. L. (1985) · The Journal of Immunology · doi:10.4049/jimmunol.135.5.3172 · Cited by 1,145
Cloning and Expression of the Human Interleukin-6 (BSF-2/IFNβ 2) Receptor
Yamasaki, K., Taga, T., Hirata, Y., Yawata, H., Kawanishi, Y., Seed, B., Taniguchi, T., Hirano, T., & Kishimoto, T. (1988) · Science · doi:10.1126/science.3136546 · Cited by 1,060
A human inhibitor of tumor necrosis factor alpha.
Seckinger, P., Isaaz, S., & Dayer, J. M. (1988) · The Journal of experimental medicine · doi:10.1084/jem.167.4.1511 · Cited by 255
A tumor necrosis factor binding protein is present in human biological fluids
Peetre, C., Thysell, H., GRUBB, A. O., & Olsson, I. (1988) · European Journal Of Haematology · doi:10.1111/j.1600-0609.1988.tb00220.x · Cited by 115
The human interferon-gamma receptor. Purification, characterization, and preparation of antibodies.
Novick, D., Orchansky, P. L., Revel, M., & Rubinstein, M. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47439-x · Cited by 82
Self-citationNovick, D., Eshhar, Z., Fischer, D. G., Friedlander, J., & Rubinstein, M. (1983) · The EMBO Journal · doi:10.1002/j.1460-2075.1983.tb01618.x · Cited by 64
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Cited by
Targeting the IL-6/JAK/STAT3 signalling axis in cancer
Johnson, D. E., O'Keefe, R. A., & Grandis, J. R. (2018) · Nature Reviews Clinical Oncology · doi:10.1038/nrclinonc.2018.8 · Cited by 3,110
IL-6 as a keystone cytokine in health and disease
Hunter, C. A., & Jones, S. A. (2015) · Nature Immunology · doi:10.1038/ni.3153 · Cited by 2,511
Age-Associated Increased Interleukin-6 Gene Expression, Late-Life Diseases, and Frailty
Ershler, W. B., & Keller, E. T. (2000) · Annual Review of Medicine · doi:10.1146/annurev.med.51.1.245 · Cited by 1,282
Rose-John, S. (2012) · International Journal of Biological Sciences · doi:10.7150/ijbs.4989 · Cited by 1,040
“Interestingly, a soluble form of the IL-6R (sIL-6R) has been found in body fluids such as urine and blood [13] and it was shown that in humans, the sIL-6R could be generated by two different mechanisms, limited proteolysis of the membrane bound receptor by the metalloprotease ADAM17 [14] and by translation of a differ…”
BackgroundMolecular cloning and expression of a receptor for human tumor necrosis factor
Schall, T. J., Lewis, M., Koller, K. J., Lee, A., Rice, G. C., Wong, G. H. W., Gatanaga, T., Granger, G. A., Lentz, R., Raab, H., et al. (1990) · Cell · doi:10.1016/0092-8674(90)90816-w · Cited by 964
Metadata
- Authors
- Novick, D., Engelmann, H., Wallach, D., & Rubinstein, M.
- Venue
- The Journal of experimental medicine
- Publisher
- Rockefeller University Press
- Published
- 1989-10-01
- Volume
- 170
- Issue
- 4
- Pages
- 1409-1414
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W2120486924
- Cited by
- 434
- References
- 9
- FWCI
- 17.43
- Type
- journal article
- Language
- en
- Open access
- green
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- Novick, D., Engelmann, H., Wallach, D., & Rubinstein, M. (1989). Soluble cytokine receptors are present in normal human urine. The Journal of experimental medicine. https://doi.org/10.1084/jem.170.4.1409
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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