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Cited by 657

The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.

Dendritic cells pulsed with protein antigens in vitro can prime antigen-specific, MHC-restricted T cells in situ.

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Inaba, K, Metlay, J P, Crowley, M T, Steinman, R M·1990·The Journal of experimental medicine
DOI 10.1084/jem.172.2.631
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Abstract

T cells recognize peptides that are bound to MHC molecules on the surface of different types of antigen-presenting cells (APC). Antigen presentation most often is studied using T cells that have undergone priming in situ, or cell lines that have been chronically stimulated in vitro. The use of primed cells provides sufficient numbers of antigen-reactive lymphocytes for experimental study. A more complete understanding of immunogenicity, however, requires that one develop systems for studying the onset of a T cell response from unprimed lymphocytes, especially in situ. Here it is shown that mouse T cells can be reliably primed in situ using dendritic cells as APC. The dendritic cells were isolated from spleen, pulsed with protein antigens, and then administered to naive mice. Antigen-responsive T cells developed in the draining lymphoid tissue, and these T cells only recognized protein when presented on cells bearing the same MHC products as the original priming dendritic cells. In contrast, little or no priming was seen if antigen-pulsed spleen cells or peritoneal cells were injected. Since very small amounts of the foreign protein were visualized within endocytic vacuoles of antigen-pulsed dendritic cells, it is suggested that dendritic cells have a small but relevant vacuolar system for presenting antigens over a several day period in situ.

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Metadata

Authors
Inaba, K., Metlay, J. P., Crowley, M. T., & Steinman, R. M.
Venue
The Journal of experimental medicine
Publisher
Rockefeller University Press
Published
1990-08-01
Volume
172
Issue
2
Pages
631-640
ISSN
  • 0022-1007 (ISSN-L)
  • 1540-9538
DOI
10.1084/jem.172.2.631
OpenAlex
W2148647317
Cited by
657
References
33
FWCI
12.10
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Dendritic cells pulsed with protein antigens in vitro can prime antigen-specific, MHC-restricted T cells in situ. https://app.cochat.ai/papers/10.1084/jem.172.2.631
The paper
Inaba, K., Metlay, J. P., Crowley, M. T., & Steinman, R. M. (1990). Dendritic cells pulsed with protein antigens in vitro can prime antigen-specific, MHC-restricted T cells in situ. The Journal of experimental medicine. https://doi.org/10.1084/jem.172.2.631

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.