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Cited by 360

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Recombinant Galectin-1 and Its Genetic Delivery Suppress Collagen-Induced Arthritis via T Cell Apoptosis

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Rabinovich, Gabriel A., Daly, Gordon, Dreja, Hanna, Tailor, Hitakshi, Riera, Clelia M., Hirabayashi, Jun, Chernajovsky, Yuti·1999·The Journal of Experimental Medicine
DOI 10.1084/jem.190.3.385
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Abstract

Galectin-1 (GAL-1), a member of a family of conserved β-galactoside–binding proteins, has been shown to induce in vitro apoptosis of activated T cells and immature thymocytes. We assessed the therapeutic effects and mechanisms of action of delivery of GAL-1 in a collagen-induced arthritis model. A single injection of syngeneic DBA/1 fibroblasts engineered to secrete GAL-1 at the day of disease onset was able to abrogate clinical and histopathological manifestations of arthritis. This effect was reproduced by daily administration of recombinant GAL-1. GAL-1 treatment resulted in reduction in anticollagen immunoglobulin (Ig)G levels. The cytokine profile in draining lymph node cells and the anticollagen IgG isotypes in mice sera at the end of the treatment clearly showed inhibition of the proinflammatory response and skewing towards a type 2–polarized immune reaction. Lymph node cells from mice engaged in the gene therapy protocol increased their susceptibility to antigen-induced apoptosis. Moreover, GAL-1–expressing fibroblasts and recombinant GAL-1 revealed a specific dose-dependent inhibitory effect in vitro in antigen-dependent interleukin 2 production to an Aq-restricted, collagen type 2–specific T cell hybridoma clone. Thus, a correlation between the apoptotic properties of GAL-1 in vitro and its immunomodulatory properties in vivo supports its therapeutic potential in the treatment of T helper cell type 1–mediated autoimmune disorders.

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Metadata

Authors
Rabinovich, G. A., Daly, G., Dreja, H., Tailor, H., Riera, C. M., Hirabayashi, J., & Chernajovsky, Y.
Venue
The Journal of Experimental Medicine
Publisher
Rockefeller University Press
Published
1999-08-02
Volume
190
Issue
3
Pages
385-398
ISSN
  • 0022-1007 (ISSN-L)
  • 1540-9538
DOI
10.1084/jem.190.3.385
OpenAlex
W2043763521
Cited by
360
References
1
FWCI
5.66
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Recombinant Galectin-1 and Its Genetic Delivery Suppress Collagen-Induced Arthritis via T Cell Apoptosis. https://app.cochat.ai/papers/10.1084/jem.190.3.385
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Rabinovich, G. A., Daly, G., Dreja, H., Tailor, H., Riera, C. M., Hirabayashi, J., & Chernajovsky, Y. (1999). Recombinant Galectin-1 and Its Genetic Delivery Suppress Collagen-Induced Arthritis via T Cell Apoptosis. The Journal of Experimental Medicine. https://doi.org/10.1084/jem.190.3.385

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