Inhibition of Vancomycin‐Resistant Enterococci by an In Vitro Continuous‐Flow Competitive Exclusion Culture Containing Human Stool Flora
Abstract
An in vitro anaerobic continuous-flow competitive exclusion (CFCE) culture model was used to study the ability of human stool flora to inhibit the growth of vancomycin-resistant (VR) enterococci (VRE). The CFCE culture was established from a stool sample obtained from a healthy adult. When 10(3) or 10(6) cfu/mL of VR Enterococcus faecium were added to the CFCE culture, the VRE were eliminated within 6 or 9 days, respectively. When 10(7) cfu/mL of the CFCE culture was added to a continuous-flow culture that contained 10(7) cfu/mL of VRE, the density of VRE was reduced but not eliminated. These data support the hypothesis that the indigenous intestinal flora inhibit growth of VRE and suggest that CFCE cultures may be a useful means to study interactions between the indigenous flora and VRE.
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Nutrient availability significantly impacts the diversity and abundance of the micro-organisms that colonize the intestine.
“Nutrient availability significantly impacts the diversity and abundance of the micro-organisms that colonize the intestine [58–63].”
From Role of the gut microbiota in nutrient competition and protection against intestinal pathogen colonization (2023) · cites this paper as [63]
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- Authors
- Donskey, C. J., Hume, M. E., Callaway, T. R., Das, S. M., Hoyen, C. K., & Rice, L. B.
- Affiliations
- Louis Stokes Cleveland VA Medical Center (US)
- Agricultural Research Service (US)
- Rainbow Babies & Children's Hospital (US)
- Venue
- The Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-12-15
- Volume
- 184
- Issue
- 12
- Pages
- 1624-1627
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- OpenAlex
- W2152226385
- Cited by
- 16
- References
- 15
- Citations by year
- 2023: 1 · 2019: 1 · 2017: 2 · 2012: 1
- FWCI
- 0.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Infectious Diseases (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Keywords
- vancomycin-resistant enterococci, Enterococcus faecium, competitive exclusion, continuous flow culture, intestinal bacteria, antimicrobial resistance, in vitro culture model, bacterial growth inhibition
- MeSH terms
- Anaerobiosis, Antibiosis, Bacteria, Bacteriological Techniques, Culture Media, Feces, Humans, Colony Count, Microbial, Enterococcus faecium, Vancomycin Resistance
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