Development of a Cocktail of Recombinant‐Expressed Human Rabies Virus–Neutralizing Monoclonal Antibodies for Postexposure Prophylaxis of Rabies
Abstract
To provide a cost-effective and safe replacement for human rabies immunoglobulin (HRIG), we used DNA recombinant technology to express 3 human rabies virus-neutralizing human monoclonal antibodies (huMAbs) in a rhabdovirus vector (RhV). Infection of either baby hamster kidney cells or CHO cells, with the resulting RhV-huMAb recombinant viruses, yielded high-level production (< or =40 micro g/mL/48 h) of RhV recombinant-expressed huMAbs (rhuMAbs) that differ in both isotype and epitope-recognition specificity. A cocktail of these rhuMAbs neutralizes several fixed and street wild-type rabies viruses (RVs). Mice and hamsters treated only once with this rhuMAb cocktail after infection with a lethal dose of RV were protected. In the mouse models, the postexposure prophylaxis (PEP) efficacy obtained with the rhuMAb cocktail was comparable to that obtained with HRIG, a finding strongly suggesting that rhuMAbs should be given serious consideration for use in future PEP of humans.
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The monoclonal antibody S057 (CR57) provides broad neutralization of a variety of rabies virus strains via binding to glycoprotein G.
“we selected the monoclonal antibody S057, also well known as CR57, one component of the published mAb cocktail, because it provides broad neutralization of a variety of rabies virus strains via binding to glycoprotein G.”
From mRNA mediates passive vaccination against infectious agents, toxins, and tumors (2017) · cites this paper as Prosniak et al, 2003
The passage labeled Abstract and Table 1 identify SO57 as a cocktail component and report neutralization of several fixed and street rabies-virus strains. Our copy does not identify SO57 as CR57 or say that its neutralization occurs through binding to rabies-virus glycoprotein G; its glycoprotein discussion concerns the expression vector instead. Page numbers were not supplied. (LLM, conf=0.86)
Experimental — accuracy not yet measuredIn 2003, a cocktail of mAbs was developed which protected Syrian hamsters from lethal challenges with a highly virulent rabies virus strain in post-exposure scenarios.
“In 2003, a cocktail of mAbs was developed which protected Syrian hamsters from lethal challenges with a highly virulent rabies virus strain in post‐exposure scenarios (Prosniak et al, 2003).”
From mRNA mediates passive vaccination against infectious agents, toxins, and tumors (2017) · cites this paper as Prosniak et al, 2003
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Cites
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Self-citationUse of mouse anti-rabies monoclonal antibodies in postexposure treatment of rabies.
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Research works printed but not linked: 1
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mRNA mediates passive vaccination against infectious agents, toxins, and tumors
Thran, M., Mukherjee, J., Pönisch, M., Fiedler, K., Thess, A., Mui, B. L., Hope, M. J., Tam, Y. K., Horscroft, N., Heidenreich, R., et al. (2017) · EMBO Molecular Medicine · doi:10.15252/emmm.201707678 · Cited by 166 · see citing works
“1) have been published previously (Prosniak et al, 2003).”
BackgroundMonoclonal antibodies for prophylactic and therapeutic use against viral infections
Both, L., Banyard, A. C., van Dolleweerd, C., Wright, E., Ma, J. K.-C., & Fooks, A. R. (2013) · Vaccine · doi:10.1016/j.vaccine.2013.01.025 · Cited by 107 · see citing works
Genetic delivery of an anti-RSV antibody to protect against pulmonary infection with RSV
Skaricic, D., Traube, C., De, B., Joh, J., Boyer, J., Crystal, R. G., & Worgall, S. (2008) · Virology · doi:10.1016/j.virol.2008.04.016 · Cited by 54 · see citing works
- Authors
- Prosniak, M., Faber, M., Hanlon, C. A., Rupprecht, C. E., Hooper, D. C., & Dietzschold, B.
- Affiliations
- Thomas Jefferson University (US)
- Centers for Disease Control and Prevention (US)
- Corresponding author
- Dietzschold, Bernhard
- Venue
- The Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-07
- Volume
- 188
- Issue
- 1
- Pages
- 53-56
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- OpenAlex
- W2123301882
- Cited by
- 97
- References
- 12
- Citations by year
- 2025: 8 · 2024: 2 · 2023: 5 · 2022: 4 · 2021: 1 · 2020: 3 · 2018: 3 · 2017: 6 · 2016: 2 · 2015: 2 · 2014: 8 · 2013: 8 · 2012: 4
- FWCI
- 1.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Infectious Diseases (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (AI 450079)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › Rabies epidemiology and control
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Keywords
- rabies virus, post-exposure prophylaxis, human monoclonal antibodies, recombinant antibodies, antibody cocktail, neutralizing antibodies, CHO cell expression
- MeSH terms
- Animals, Antibodies, Monoclonal, Antibodies, Viral, Antibody Specificity, Cell Line, Female, Genetic Engineering, Cricetinae, Humans, Immunization, Passive, Neutralization Tests, Rabies, Rabies virus, Recombinant Proteins, Gene Expression, Mice
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