Cell‐Wall Alterations as an Attribute of Mycobacterium tuberculosis in Latent Infection
Abstract
Ziehl-Neelsen (ZN) staining is the key technique for diagnosis of mycobacterial infections; however, a high percentage of patients exhibit positive signs of tuberculosis, as indicated by pathology, culture of mycobacteria, and polymerase chain-reaction analysis, and yet show negative results on ZN staining. In this report we present evidence that such ZN-negative specimens represent Mycobacterium tuberculosis bacilli in a dormant state with distinct cell-wall alterations: the classical cell-wall composition-dependent ZN staining of M. tuberculosis in lung sections gradually discontinued with persistence of infection, both in mice and in human patients; in contrast, detection of mycobacteria by cell-wall composition-independent staining using a polyclonal anti-M. bovis Bacille-Calmette-Guérin serum continued with persistence of infection. These findings have important implications for diagnosis, as well as for both chemotherapy and development of vaccine strategies.'
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Regulation of the cell wall affects immune responses and determines sensitivity to antibiotics.
“regulation of the cell wall affects immune responses13 (Box 1) and determines sensitivity to antibiotics14–18 (Box 2).”
From The mycobacterial cell envelope — a moving target (2020) · cites this paper as [16]
M. tuberculosis cells in latent infection have a cell wall that is different from that observed during active infection.
“This observational study with human and mouse tissue sections shows that M. tuberculosis cells in latent infection have a cell wall that is different from that observed during active infection.”
From The mycobacterial cell envelope — a moving target (2020) · cites this paper as [16]
Loss of acid-fastness has also been observed in latent infections in humans and chronic stage infections in mice; it is possible that this could be mediated through regulation of KasB.
“Loss of acid-fastness has also been observed in latent infections in humans and chronic stage infections in mice16; it is possible that this could be mediated through regulation of KasB.”
From The mycobacterial cell envelope — a moving target (2020) · cites this paper as [16]
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Modeling of Mycobacterium tuberculosis dormancy in bacterial cultures.
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Cytokinin signaling alters acid-fast staining of Mycobacterium tuberculosis
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Superior protection elicited by live-attenuated vaccines in the murine model of paratuberculosis.
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“to compare the vaccine potential of the sigL mutant to that of the leuD mutant because tissue colonization levels in the leuD experiments were analyzed using only Ziehl-Neelsen staining (55, 56), a less sensitive assay than the counting of CFU, used in our study.”
Method
- Authors
- Seiler, P., Ulrichs, T., Bandermann, S., Pradl, L., Jörg, S., Krenn, V., Morawietz, L., Kaufmann, S. H. E., & Aichele, P.
- Affiliations
- Max Planck Society (DE)
- Charité – Universitätsmedizin Berlin (DE)
- Corresponding author
- Seiler, Peter
- Venue
- The Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-11
- Volume
- 188
- Issue
- 9
- Pages
- 1326-1331
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- OpenAlex
- W1979690999
- Cited by
- 157
- References
- 0
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 5 · 2023: 3 · 2022: 8 · 2021: 5 · 2020: 6 · 2019: 12 · 2018: 6 · 2017: 9 · 2016: 7 · 2015: 9 · 2014: 14 · 2013: 7 · 2012: 10
- FWCI
- 4.69
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Infectious Diseases (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- FreiDok plus (Universitätsbibliothek Freiburg) (submittedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Health Sciences › Medicine › Surgery › Infectious Diseases and Tuberculosis
- Keywords
- Mycobacterium tuberculosis, latent tuberculosis infection, Ziehl-Neelsen staining, dormant mycobacteria, persistent infection, tuberculosis diagnosis, tuberculosis vaccine development
- MeSH terms
- Animals, Bacterial Proteins, BCG Vaccine, Cell Wall, Female, Humans, Immunohistochemistry, Lung, Male, Mice, Inbred C57BL, Mycobacterium tuberculosis, Rosaniline Dyes, Staining and Labeling, Tuberculosis, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
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