Fundamentals and application of magnetic particles in cell isolation and enrichment: a review
Open accessAbstract
Magnetic sorting using magnetic beads has become a routine methodology for the separation of key cell populations from biological suspensions. Due to the inherent ability of magnets to provide forces at a distance, magnetic cell manipulation is now a standardized process step in numerous processes in tissue engineering, medicine, and in fundamental biological research. Herein we review the current status of magnetic particles to enable isolation and separation of cells, with a strong focus on the fundamental governing physical phenomena, properties and syntheses of magnetic particles and on current applications of magnet-based cell separation in laboratory and clinical settings. We highlight the contribution of cell separation to biomedical research and medicine and detail modern cell-separation methods (both magnetic and non-magnetic). In addition to a review of the current state-of-the-art in magnet-based cell sorting, we discuss current challenges and available opportunities for further research, development and commercialization of magnetic particle-based cell-separation systems.
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MACS is extensively used in preclinical and clinical cancer research and for sorting white blood cell populations and stem cells.
“MACS is extensively used in preclinical and clinical cancer research, as well as for sorting white blood cell populations and stem cells [201].”
From Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications (2019) · cites this paper as [201]
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Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications
Dadfar, S. M., Roemhild, K., Drude, N. I., von Stillfried, S., Knüchel, R., Kiessling, F., & Lammers, T. (2019) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2019.01.005 · Cited by 1,181 · see citing works
- Authors
- Plouffe, B. D., Murthy, S. K., & Lewis, L. H.
- Affiliations
- Northeastern University (US)
- Corresponding author
- Lewis, Laura H
- Venue
- Reports on Progress in Physics
- Publisher
- IOP Publishing
- Published
- 2014-12-04
- Volume
- 78
- Issue
- 1
- Pages
- 016601
- ISSN
- 0034-4885 (ISSN-L)
- 1361-6633
- PMC
- PMC4310825
- OpenAlex
- W2031386013
- Cited by
- 334
- References
- 5
- Citations by year
- 2026: 20 · 2025: 21 · 2024: 24 · 2023: 21 · 2022: 34 · 2021: 38 · 2020: 42 · 2019: 40 · 2018: 30 · 2017: 27 · 2016: 27 · 2015: 9 · 2013: 1
- FWCI
- 1.81
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Reports on Progress in Physics (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Richard Saferstein
- National Institutes of Health (R01-EB009327)
- Division of Graduate Education (NSF-DGE-0504331)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Physical Sciences › Engineering › Computational Mechanics › Field-Flow Fractionation Techniques
- Keywords
- magnetic particles, magnetic cell separation, magnetic beads, cell isolation, cell enrichment, magnetic force, tissue engineering
- MeSH terms
- Animals, Cell Separation, Humans, Magnets, Magnetic Phenomena
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