Fluorescence lifetime imaging microscopy in life sciences
Open accessAbstract
Fluorescence lifetime imaging microscopy (FLIM) and fluorescence anisotropy imaging microscopy (FAIM) are versatile tools for the investigation of the molecular environment of fluorophores in living cells.Owing to nanometre-scale interactions via Förster resonance energy transfer (FRET), FLIM and FAIM are powerful microscopy methods for the detection of conformational changes and protein-protein interactions reflecting the biochemical status of live cells.This review provides an overview of recent advances in photonics techniques, quantitative data analysis methods and applications in the life sciences.
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Developments in experimental techniques such as ultrafast spectroscopy, single molecule spectroscopy, time-resolved microscopy and single particle imaging have enabled study of biological dynamics on increasingly small length and time scales, revealing processes necessary for living function that depend on a delicate interplay between quantum and classical physical effects.
“Recently, developments in experimental techniques such as ultrafast spectroscopy [6], single molecule spectroscopy [7 -11], timeresolved microscopy [12 -14] and single particle imaging [15 -18] have enabled us to study biological dynamics on increasingly small length and time scales, revealing a variety of processes necessary for the function of the living system that depend on a delicate interpl”
From The future of quantum biology (2018) · cites this paper as [14]
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References 160 · Cited by 168
- Authors
- Borst, J. W., & Visser, A. J. W. G.
- Affiliations
- Wageningen University & Research (NL)
- ORCID
- Borst, Jan Willem
- Venue
- Measurement Science and Technology
- Publisher
- IOP Publishing
- Published
- 2010-09-14
- Volume
- 21
- Issue
- 10
- Pages
- 102002
- ISSN
- 0957-0233 (ISSN-L)
- 1361-6501
- OpenAlex
- W2006944854
- Cited by
- 168
- References
- 160
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 9 · 2023: 9 · 2022: 5 · 2021: 10 · 2020: 12 · 2019: 6 · 2018: 9 · 2017: 9 · 2016: 14 · 2015: 12 · 2014: 18 · 2013: 25 · 2012: 11
- FWCI
- 8.69
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Measurement Science and Technology (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Photochemistry and Electron Transfer Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Keywords
- fluorescence lifetime imaging, fluorescence resonance energy transfer, protein-protein interaction, conformational change, live-cell imaging, quantitative data analysis
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