Cell cycle-dependent alteration in NAC1 nuclear body dynamics and morphology
Open accessAbstract
NAC1, a BTB/POZ family member, has been suggested to participate in maintaining the stemness of embryonic stem cells and has been implicated in the pathogenesis of human cancer. In ovarian cancer, NAC1 upregulation is associated with disease aggressiveness and with the development of chemoresistance. Like other BTB/POZ proteins, NAC1 forms discrete nuclear bodies in non-dividing cells. To investigate the biological role of NAC1 nuclear bodies, we characterized the expression dynamics of NAC1 nuclear bodies during different phases of the cell cycle. Fluorescence recovery after photobleaching assays revealed that NAC1 was rapidly exchanged between the nucleoplasm and NAC1 nuclear bodies in interphase cells. The number of NAC1 bodies significantly increased and their size decreased in the S phase as compared to the G₀/G₁ and G₂ phases. NAC1 nuclear bodies disappeared and NAC1 became diffuse during mitosis. NAC1 nuclear bodies reappeared immediately after completion of mitosis. These results indicate that a cell cycle-dependent regulatory mechanism controls NAC1 body formation in the nucleus and suggest that NAC1 body dynamics are associated with mitosis or cytokinesis.
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DNA content and cell cycle phases can be identified from intensity of fluorescently labeled nuclei in images.
“It has been shown in our previous studies that DNA content and cell cycle phases can be identified from the intensity of fluorescently labeled nuclei in images [24–26].”
From Senescent cell heterogeneity and responses to senolytic treatment are related to cell cycle status during senescence induction (2025) · cites this paper as [25]
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- Authors
- Wu, P.-H., Hung, S.-H., Ren, T., Shih, I.-M., & Tseng, Y.
- Affiliations
- University of Florida (US)
- Johns Hopkins University (US)
- Venue
- Physical Biology
- Publisher
- IOP Publishing
- Published
- 2011-02-01
- Volume
- 8
- Issue
- 1
- Pages
- 015005
- ISSN
- 1478-3967 (ISSN-L)
- 1478-3975
- PMC
- PMC3164573
- OpenAlex
- W2036579115
- Cited by
- 32
- References
- 1
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 5 · 2021: 1 · 2020: 4 · 2019: 1 · 2017: 3 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 4 · 2012: 2
- FWCI
- 1.23
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Physical Biology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Cancer Institute (U54CA143868, CA-103937, R01CA103937)
- National Institute of Biomedical Imaging and Bioengineering (R01EB004416)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- NAC1, nuclear bodies, cell cycle, mitosis, cytokinesis, FRAP, ovarian cancer, chemoresistance
- MeSH terms
- Cell Cycle, Cell Nucleus, Female, Humans, Neoplasm Proteins, Neoplasms, Ovarian Neoplasms, Repressor Proteins, Cell Line, Tumor
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