In vitro and in vivo evaluation of bone formation using solid freeform fabrication-based bone morphogenic protein-2 releasing PCL/PLGA scaffolds
Abstract
The aim of this study was to develop novel polycaprolactone/poly(lactic-co-glycolic acid) (PCL/PLGA) scaffolds with a heparin-dopamine (Hep-DOPA) conjugate for controlled release of bone morphogenic protein-2 (BMP-2) to enhance osteoblast activity in vitro and also bone formation in vivo. PCL/PLGA scaffolds were prepared by a solid freeform fabrication method. The PCL/PLGA scaffolds were functionalized with Hep-DOPA and then BMP-2 was sequentially coated onto the Hep-DOPA/PCL/PLGA scaffolds. The characterization and surface elemental composition of all scaffolds were evaluated by scanning electron microscope and x-ray photoelectron spectroscopy. The osteoblast activities on all scaffolds were assessed by cell proliferation, alkaline phosphatase (ALP) activity and calcium deposition in vitro. To demonstrate bone formation in vivo, plain radiograph, micro-computed tomography (micro-CT) evaluation and histological studies were performed after the implantation of all scaffolds on a rat femur defect. Hep-DOPA/PCL/PLGA had more controlled release of BMP-2, which was quantified by enzyme-linked immunosorbent assay, compared with Hep/PCL/PLGA. The in vitro results showed that osteoblast-like cells (MG-63 cells) grown on BMP-2/Hep-DOPA/PCL/PLGA had significantly enhanced ALP activity and calcium deposition compared with those on BMP-2/Hep/PCL/PLGA and PCL/PLGA. In addition, the plain radiograph, micro-CT evaluation and histological studies demonstrated that the implanted BMP-2/Hep-DOP…
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Upon immobilization of BMP, the property of osteoblast cells responsive to polymeric materials can be remarkably improved.
“Upon immobilization of BMP, the property of osteoblast cells responsive to polymeric materials can be remarkably improved”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [95]
Material mineralization can be expedited in the presence of immobilized growth factors.
“and the material mineralization can be expedited in the presence of immobilized growth factors [91–95].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [95]
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Polydopamine-Assisted Surface Modification for Bone Biosubstitutes
Huang, S., Liang, N., Hu, Y., Zhou, X., & Abidi, N. (2016) · BioMed Research International · doi:10.1155/2016/2389895 · Cited by 97 · see citing works
“[94] T.”
Background
- Authors
- Kim, T.-H., Yun, Y.-P., Park, Y.-E., Lee, S.-H., Yong, W., Kundu, J., Jung, J. W., Shim, J.-H., Cho, D.-W., Kim, S. E., & Song, H.-R.
- Affiliations
- Konkuk University (KR)
- Korea University (KR)
- Pohang University of Science and Technology (KR)
- ORCID
- Venue
- Biomedical Materials
- Publisher
- IOP Publishing
- Published
- 2014-02-11
- Volume
- 9
- Issue
- 2
- Pages
- 025008
- ISSN
- 1748-6041 (ISSN-L)
- 1748-605X
- OpenAlex
- W2092632731
- Cited by
- 42
- References
- 0
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 5 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 6 · 2018: 3 · 2017: 4 · 2016: 6 · 2015: 2 · 2014: 4
- FWCI
- 2.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Biomedical Materials (publishedVersion)
- PubMed (publishedVersion)
- Open Access System for Information Sharing (Pohang University of Science and Technology) (submittedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Dentistry › Oral Surgery › Dental Implant Techniques and Outcomes
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Keywords
- BMP-2, additive manufacturing, osteoblast activity, bone formation, bone tissue engineering, alkaline phosphatase activity, calcium deposition, MG-63 cells
- MeSH terms
- Polylactic Acid-Polyglycolic Acid Copolymer, Animals, Biocompatible Materials, Bone and Bones, Calcium, Dihydroxyphenylalanine, Dopamine, Enzyme-Linked Immunosorbent Assay, Femur, Heparin, Humans, Osteoblasts, Osteogenesis, Polyglycolic Acid, Surface Properties, Lactic Acid, Cell Line, Tumor, Cell Proliferation, Rats, X-Ray Microtomography, Bone Morphogenetic Protein 2, Photoelectron Spectroscopy
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