The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Extracellular Matrix Mineralization and Osteoblast Gene Expression by Human Adipose Tissue–Derived Stromal Cells
Abstract
Human adipose tissue represents an abundant reservoir of stromal cells with potential utility for tissue engineering. The current study demonstrates the ability of human adipose tissue-derived stromal cells to display some of the hallmarks of osteoblast differentiation in vitro. Following treatment with ascorbate, beta-glycerophosphate, dexamethasone, and 1,25 dihydroxy vitamin D(3), adipose tissue-derived stromal cells mineralize their extracellular matrix based on detection of calcium phosphate deposits using Alizarin Red and von Kossa histochemical stains. Fourier transform infrared analysis demonstrates the apatitic nature of these crystals. Mineralization is accompanied by increased expression or activity of the osteoblast-associated proteins osteocalcin and alkaline phosphatase. These and other osteoblast-associated gene markers are detected based on polymerase chain reaction. In contrast, the adipocyte gene markers--leptin, lipoprotein lipase, and peroxisome proliferator activated receptor gamma2--are reduced under mineralization conditions, consistent with the reciprocal relationship postulated to exist between adipocytes and osteoblasts. The current work supports the presence of a multipotent stromal cell population within human extramedullary adipose tissue. These findings have potential implications for human bone tissue bioengineering.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 46,426
Multilineage potential of adult human mesenchymal stem cells
Pittenger, M. F., Mackay, A. M., Beck, S. C., Jaiswal, R. K., Douglas, R., Mosca, J. D., Moorman, M. A., Simonetti, D. W., Craig, S., & Marshak, D. R. (1999) · Science · doi:10.1126/science.284.5411.143 · Cited by 21,052
Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based Therapies
Zuk, P. A., Zhu, M., Mizuno, H., Huang, J., Futrell, J. W., Katz, A. J., Benhaim, P., Lorenz, H. P., & Hedrick, M. H. (2001) · Tissue Engineering · doi:10.1089/107632701300062859 · Cited by 7,956
Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues
Prockop, D. J. (1997) · Science · doi:10.1126/science.276.5309.71 · Cited by 4,629
Green, H., & Kehinde, O. (1975) · Cell · doi:10.1016/0092-8674(75)90087-2 · Cited by 1,360
Theory and Practice of Histotechnology
Sheehan, D. C., & Hrapchak, B. B. (1973) · Medical Entomology and Zoology · Cited by 1,128
Beresford, J. N., Bennett, J. H., Devlin, C., Leboy, P. S., & Owen, M. E. (1992) · Journal of Cell Science · doi:10.1242/jcs.102.2.341 · Cited by 836
MEUNIER, P., AARON, J., EDOUARD, C., & VlGNON, G. (1971) · Clinical Orthopaedics and Related Research · doi:10.1097/00003086-197110000-00021 · Cited by 779
Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells
Conget, P. A., & Minguell, J. J. (1999) · Journal of Cellular Physiology · doi:10.1002/(sici)1097-4652(199910)181:1<67::aid-jcp7>3.0.co;2-c · Cited by 711
Grigoriadis, A. E., Heersche, J. N., & Aubin, J. E. (1988) · The Journal of cell biology · doi:10.1083/jcb.106.6.2139 · Cited by 649
Cited by
Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based Therapies
Zuk, P. A., Zhu, M., Mizuno, H., Huang, J., Futrell, J. W., Katz, A. J., Benhaim, P., Lorenz, H. P., & Hedrick, M. H. (2001) · Tissue Engineering · doi:10.1089/107632701300062859 · Cited by 7,956
Adipose-derived stem cells for regenerative medicine
Gimble, J. M., Katz, A. J., & Bunnell, B. A. (2007) · Circulation Research · doi:10.1161/01.res.0000265074.83288.09 · Cited by 2,332
Self-citationStem cells in tissue engineering
Bianco, P., & Robey, P. G. (2001) · Nature · doi:10.1038/35102181 · Cited by 965
Adipose-derived adult stromal cells heal critical-size mouse calvarial defects
Cowan, C. M., Shi, Y.-Y., Aalami, O. O., Chou, Y.-F., Mari, C., Thomas, R., Quarto, N., Contag, C. H., Wu, B., & Longaker, M. T. (2004) · Nature Biotechnology · doi:10.1038/nbt958 · Cited by 914
The Potential of Adipose Stem Cells in Regenerative Medicine
Lindroos, B., Suuronen, R., & Miettinen, S. (2011) · Stem Cell Reviews and Reports · doi:10.1007/s12015-010-9193-7 · Cited by 441
Metadata
- Authors
- Halvorsen, Y.-D. C., Franklin, D., Bond, A. L., Hitt, D. C., Auchter, C., Boskey, A. L., Paschalis, E. P., Wilkison, W. O., & Gimble, J. M.
- Venue
- Tissue Engineering
- Publisher
- SAGE Publications
- Published
- 2001-12
- Volume
- 7
- Issue
- 6
- Pages
- 729-741
- ISSN
- 1076-3279 (ISSN-L)
- 1557-8690
- OpenAlex
- W2087255769
- Cited by
- 508
- References
- 45
- FWCI
- 6.03
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Extracellular Matrix Mineralization and Osteoblast Gene Expression by Human Adipose Tissue–Derived Stromal Cells. https://app.cochat.ai/papers/10.1089/107632701753337681
- The paper
- Halvorsen, Y.-D. C., Franklin, D., Bond, A. L., Hitt, D. C., Auchter, C., Boskey, A. L., Paschalis, E. P., Wilkison, W. O., & Gimble, J. M. (2001). Extracellular Matrix Mineralization and Osteoblast Gene Expression by Human Adipose Tissue–Derived Stromal Cells. Tissue Engineering. https://doi.org/10.1089/107632701753337681
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
