The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Effects of Wnt Signaling on Proliferation and Differentiation of Human Mesenchymal Stem Cells
Abstract
Mesenchymal stem cells are pluripotent cells from bone marrow, which can be differentiated into the osteogenic, chondrogenic, and adipogenic lineages in vitro and are a source of cells in bone and cartilage tissue engineering. An improvement in current tissue-engineering protocols requires more detailed insight into the molecular cues that regulate the distinct steps of osteochondral differentiation. Because Wnt signaling has been widely implicated in mesenchymal differentiation, we analyzed the role of Wnt signaling in human mesenchymal stem cell (hMSC) biology by stimulation of the pathway with lithium chloride and Wnt3A-conditioned medium. We demonstrate a role for low levels of Wnt signaling in proliferation of uncommitted hMSCs and confirm that Wnt signaling controls osteoprogenitor proliferation. On the other hand, at high Wnt levels we observed a block in adipogenic differentiation and an increase in the expression of alkaline phosphatase, suggesting a role in the initiation of osteogenesis. The results of this study suggest that bone tissue engineering could benefit from the activation of critical levels of Wnt signaling at defined stages of differentiation. Moreover, our data suggest that hMSCs provide a valid in vitro model to study the role of Wnt signaling in mesenchymal biology.
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Cites
Multilineage potential of adult human mesenchymal stem cells
Pittenger, M. F., Mackay, A. M., Beck, S. C., Jaiswal, R. K., Douglas, R., Mosca, J. D., Moorman, M. A., Simonetti, D. W., Craig, S., & Marshak, D. R. (1999) · Science · doi:10.1126/science.284.5411.143 · Cited by 21,052
Wnt signaling: a common theme in animal development
Cadigan, K. M., & Nusse, R. (1997) · Genes & Development · doi:10.1101/gad.11.24.3286 · Cited by 2,567
A molecular mechanism for the effect of lithium on development.
Klein, P. S., & Melton, D. A. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.16.8455 · Cited by 2,301
LDL Receptor-Related Protein 5 (LRP5) Affects Bone Accrual and Eye Development
Gong, Y., Slee, R. B., Fukai, N., Rawadi, G., Roman-Roman, S., Reginato, A. M., Wang, H., Cundy, T., Glorieux, F. H., Lev, D., et al. (2001) · Cell · doi:10.1016/s0092-8674(01)00571-2 · Cited by 2,235
Self-citationInhibition of Adipogenesis by Wnt Signaling
Ross, S. E., Hemati, N., Longo, K. A., Bennett, C. N., Lucas, P. C., Erickson, R. L., & MacDougald, O. A. (2000) · Science · doi:10.1126/science.289.5481.950 · Cited by 1,874
Isolation of multipotent adult stem cells from the dermis of mammalian skin
Toma, J. G., Akhavan, M., Fernandes, K. J. L., Barnabé-Heider, F., Sadikot, A., Kaplan, D. R., & Miller, F. D. (2001) · Nature Cell Biology · doi:10.1038/ncb0901-778 · Cited by 1,593
High Bone Density Due to a Mutation in LDL-Receptor–Related Protein 5
Boyden, L. M., Mao, J., Belsky, J., Mitzner, L., Farhi, A., Mitnick, M. A., Wu, D., Insogna, K., & Lifton, R. P. (2002) · New England Journal of Medicine · doi:10.1056/nejmoa013444 · Cited by 1,591
Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats
Maniatopoulos, C., Sodek, J., & Melcher, A. H. (1988) · Cell and Tissue Research · doi:10.1007/bf00225804 · Cited by 1,324
Characterization of cells with osteogenic potential from human marrow
Haynesworth, S. E., Goshima, J., Goldberg, V. M., & Caplan, A. I. (1992) · Bone · doi:10.1016/8756-3282(92)90364-3 · Cited by 1,289
Little, R. D., Folz, C., Manning, S. P., Swain, P. M., Zhao, S.-C., Eustace, B., Lappe, M. M., Spitzer, L., Zweier, S., Braunschweiger, K., et al. (2002) · The American Journal of Human Genetics · doi:10.1086/338450 · Cited by 1,282
Cited by
Ding, D.-C., Shyu, W.-C., & Lin, S.-Z. (2011) · Cell Transplantation · doi:10.3727/096368910x · Cited by 894
Biological functions of mesenchymal stem cells and clinical implications
Naji, A., Eitoku, M., Favier, B., Deschaseaux, F., Rouas-Freiss, N., & Suganuma, N. (2019) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-019-03125-1 · Cited by 624
Bioinorganics and biomaterials: Bone repair
Habibovic, P., & Barralet, J. E. (2011) · Acta Biomaterialia · doi:10.1016/j.actbio.2011.03.027 · Cited by 441
Wnt signaling controls the fate of mesenchymal stem cells
Ling, L., Nurcombe, V., & Cool, S. M. (2009) · Gene · doi:10.1016/j.gene.2008.12.008 · Cited by 417
miR-29 Modulates Wnt Signaling in Human Osteoblasts through a Positive Feedback Loop
Kapinas, K., Kessler, C., Ricks, T., Gronowicz, G., & Delany, A. M. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.116137 · Cited by 396
Metadata
- Authors
- de Boer, J., Wang, H. J., & van Blitterswijk, C.
- Venue
- Tissue Engineering
- Publisher
- SAGE Publications
- Published
- 2004-03
- Volume
- 10
- Issue
- 3-4
- Pages
- 393-401
- ISSN
- 1076-3279 (ISSN-L)
- 1557-8690
- OpenAlex
- W2029143606
- Cited by
- 275
- References
- 49
- FWCI
- 8.31
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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