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Toward Optimal Cryopreservation and Storage for Achievement of High Cell Recovery and Maintenance of Cell Viability and T Cell Functionality

Angel, Stephanie, von Briesen, Hagen, Oh, Young-Joo, Baller, Marko K., Zimmermann, Heiko, Germann, Anja·2016·Biopreservation and Biobanking
DOI 10.1089/bio.2016.0046PMC PMC5180082
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Abstract

Cryopreservation of biological materials such as cells, tissues, and organs is a prevailing topic of high importance. It is employed not only in many research fields but also in the clinical area. Cryopreservation is of great importance for reproductive medicine and clinical studies, as well as for the development of vaccines. Peripheral blood mononuclear cells (PBMCs) are commonly used in vaccine research where comparable and reliable results between different research institutions and laboratories are of high importance. Whereas freezing and thawing processes are well studied, controlled, and standardized, storage conditions are often disregarded. To close this gap, we investigated the influence of suboptimal storage conditions during low-temperature storage on PBMC viability, recovery, and T cell functionality. For this purpose, PBMCs were isolated and exposed with help of a robotic system in a low-temperature environment from 0 up to 350 temperature fluctuation cycles in steps of 50 cycles to simulate storage conditions in large biorepositories with sample storage, removal, and sorting functions. After the simulation, the viability, recovery, and T cell functionality were analyzed to determine the number of temperature rises, which ultimately lead to significant cell damage. All studied parameters decreased with increasing number of temperature cycles. Sometimes after as little as only 50 temperature cycles, a significant effect was observed. These results are very important for all fields in which cell cryopreservation is employed, particularly for clinical and multicenter studies wherein the comparability and reproducibility of results play a crucial role. To obtain reliable results and to maintain the quality of the cells, not only the freezing and thawing processes but also the storage conditions should be controlled and standardized, and any deviations should be documented.

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  • Where there is multi-user access, any storage system will be prone to temperature cycling, which is known to reduce viability, and the failure of routine liquid nitrogen refilling procedures can result in the catastrophic loss of viability of stored materials.

    “Where there is multi-user access, any storage system will be prone to temperature cycling, which is known to reduce viability (Angel et al., 2016), and the failure of routine liquid nitrogen refilling procedures can result in the catastrophic loss of viability of stored materials.”

    From Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0) (2021) · cites this paper as Angel et al., 2016

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Authors
Angel, S., von Briesen, H., Oh, Y.-J., Baller, M. K., Zimmermann, H., & Germann, A.
Affiliations
  • Fraunhofer Institute for Biomedical Engineering
  • University of Applied Sciences Kaiserslautern
Venue
Biopreservation and Biobanking
Publisher
SAGE Publications
Published
2016-10-28
Volume
14
Issue
6
Pages
539-547
ISSN
  • 1947-5535
  • 1947-5543
DOI
10.1089/bio.2016.0046
PMC
PMC5180082
Cited by
41
References
1
Language
en
Licence

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