Effects of cryopreservation on lymphocyte immunophenotype and function

Preservation of Lymphocyte Immunophenotype and Proliferative Responses in Cryopreserved Peripheral Blood Mononuclear Cells from Human Immunodeficiency Virus Type 1-Infected Donors: Implications for Multicenter Clinical Trials

Reimann, K. A., Chernoff, M., Wilkening, C. L., Nickerson, C. E., LANDAY, A. L., & Laboratories, T. A. I. A. T. (2000) · Clinical and Diagnostic Laboratory Immunology · doi:10.1128/cdli.7.3.352-359.2000 · Cited by 124 · see citing works

Viability and Recovery of Peripheral Blood Mononuclear Cells Cryopreserved for up to 12 Years in a Multicenter Study

Kleeberger, C. A., Lyles, R. H., Margolick, J. B., Rinaldo, C. R., Phair, J., & Giorgi, J. V. (1999) · Clinical and Diagnostic Laboratory Immunology · doi:10.1128/cdli.6.1.14-19.1999 · Cited by 100 · see citing works

Viability and Functional Activity of Cryopreserved Mononuclear Cells

Weinberg, A., Zhang, L., Brown, D., Erice, A., Polsky, B., Hirsch, M., Owens, S., & Lamb, K. (2000) · Clinical and Diagnostic Laboratory Immunology · doi:10.1128/cdli.7.4.714-716.2000 · Cited by 96 · see citing works

Hematopoietic potential of cryopreserved and ex vivo manipulated umbilical cord blood progenitor cells evaluated in vitro and in vivo

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Lymphocyte immunophenotyping by flow cytometry in normal adults

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Effect of Shipment, Storage, Anticoagulant, and Cell Separation on Lymphocyte Proliferation Assays for Human Immunodeficiency Virus-Infected Patients

Weinberg, A., Betensky, R. A., Zhang, L., & Ray, G. (1998) · Clinical and Diagnostic Laboratory Immunology · doi:10.1128/cdli.5.6.804-807.1998 · Cited by 83 · see citing works

Cryopreservation of human platelets with dimethyl sulfoxide: changes in biochemistry and cell function

Böck, M., Schleuning, M., Heim, M. U., & Mempel, W. (1995) · Transfusion · doi:10.1046/j.1537-2995.1995.351196110896.x · Cited by 52 · see citing works

Cryopreservation and long‐term liquid nitrogen storage of peripheral blood mononuclear cells for flow cytometry analysis effects on cell subset proportions and fluorescence intensity

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Successful cryopreservation of purified autologous CD34+ cells: influence of freezing parameters on cell recovery and engraftment

Beaujean, F., Bourhis, J.-H., Bayle, C., Jouault, H., Diviné, M., Rieux, C., Janvier, M., Forestier, C. L., & Pico, J. (1998) · Bone Marrow Transplantation · doi:10.1038/sj.bmt.1701494 · Cited by 44 · see citing works

Clonogenic capacity andex vivo expansion potential of umbilical cord blood progenitor cells are not impaired by cryopreservation

Almici, C., Carlo‐Stella, C., Wagner, J., Mangoni, L., Garau, D., Re, A., Giachetti, R., Cesana, C., & Rizzoli, V. (1997) · Bone Marrow Transplantation · doi:10.1038/sj.bmt.1700803 · Cited by 37 · see citing works

Decrease of L-selectin expression on human CD34+ cells on freeze-thawing and rapid recovery with short-term incubation

Hattori, Y., Kato, H., Nitta, M., & Takamoto, S. (2001) · Experimental Hematology · doi:10.1016/s0301-472x(00)00615-9 · Cited by 36 · see citing works

Adhesion molecules on peripheral blood-derived CD34+ cells: effects of cryopreservation and short-term ex vivo incubation with serum and cytokines

Koenigsmann, M., Koenigsmann, M., Notter, M., Neuloh, M., Mücke, C., Thiel, E., & Berdel, W. (1998) · Bone Marrow Transplantation · doi:10.1038/sj.bmt.1701484 · Cited by 34 · see citing works

Cryopreservation Modifies Flow-Cytometric Analysis of Hemopoietic Cells

Rosillo, M. C., Ortuño, F. J., Rivera, J., Moraleda, J. M., & Vicente, V. (1995) · Vox Sanguinis · doi:10.1111/j.1423-0410.1995.tb02574.x · Cited by 28 · see citing works

Differential expression ofβ1 andβ2 integrins and L-selectin on CD4+ and CD8+ T lymphocytes in human blood: comparative analysis between isolated cells, whole blood samples and cryopreserved preparations

Cavers, M., Afzali, B., Macey, M. G., McCarthy, D. A., Irshad, S., & Brown, K. A. (2002) · Clinical & Experimental Immunology · doi:10.1046/j.1365-2249.2002.01711.x · Cited by 23 · see citing works

Lymphocyte subpopulations do not alter during blood storage at 4°C

van Lambalgen, R., & Meurs, G. J. E. V. (1985) · Journal of Immunological Methods · doi:10.1016/0022-1759(85)90162-0 · Cited by 14 · see citing works

Effect of cryopreservation on lymphocyte markers evaluated by monoclonal antibodies

Glassman, A. B., & Christopher, J. B. (1984) · Transfusion · doi:10.1046/j.1537-2995.1984.24685066822.x · Cited by 13 · see citing works

Ex Vivo Expansion of Frozen/Thawed CD34+ Cells Isolated from Frozen Human Apheresis Products

Martinson, J., Loudovaris, M., Smith, S. L., BENDER, J. G., Vachula, M., Epps, D. E. V., Kaizer, H., Ghalie, R., & McLeod, B. C. (1997) · Journal of Hematotherapy · doi:10.1089/scd.1.1997.6.69 · Cited by 10 · see citing works

The Activity in ex vivo Expansion of Cord Blood Myeloid Progenitor Cells before and after Cryopreservation

Wang, S., Hsu, M., Huang, M.-Z., Hsu, H., Tzeng, C., Hung, J.-H., & Ho, C. (2001) · Acta Haematologica · doi:10.1159/000046531 · Cited by 9 · see citing works

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Cossarizza, A., Chang, H., Radbruch, A., Acs, A., Adam, D., Adam‐Klages, S., Agace, W. W., Aghaeepour, N., Akdiş, M., Allez, M., et al. (2019) · European Journal of Immunology · doi:10.1002/eji.201970107 · Cited by 982 · see citing works

Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes

Newell, E. W., Sigal, N., Bendall, S. C., Nolan, G. P., & Davis, M. M. (2012) · Immunity · doi:10.1016/j.immuni.2012.01.002 · Cited by 555 · see citing works

Guidelines for the use of flow cytometry and cell sorting in immunological studies *

Cossarizza, A., Chang, H., Radbruch, A., Akdiş, M., Andrä, I., Annunziato, F., Bächer, P., Barnaba, V., Battistini, L., Bauer, W. M., et al. (2017) · European Journal of Immunology · doi:10.1002/eji.201646632 · Cited by 572 · see citing works

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Cossarizza, A., Chang, H., Radbruch, A., Abrignani, S., Addo, R., Akdis, M., Andrä, I., Andreata, F., Annunziato, F., Arranz, E., et al. (2021) · European Journal of Immunology · doi:10.1002/eji.202170126 · Cited by 531 · see citing works

Long COVID manifests with T cell dysregulation, inflammation and an uncoordinated adaptive immune response to SARS-CoV-2

Yin, K., Peluso, M. J., Luo, X., Thomas, R., Shin, M., Neidleman, J. A., Andrew, A. K., Young, K. C., Ma, T., Hoh, R., et al. (2024) · Nature Immunology · doi:10.1038/s41590-023-01724-6 · Cited by 399 · see citing works

Depletion of Regulatory T Cells in HIV Infection Is Associated with Immune Activation

Eggena, M. P., Barugahare, B. J., Jones, N. G., Okello, M., Mutalya, S., Kityo, C. M., Mugyenyi, P. N., & Cao, H. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.174.7.4407 · Cited by 373 · see citing works

Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: position statement of the T-Cell Workshop Committee of the Immunology of Diabetes Society

Mallone, R., Mannering, S. I., Brooks‐Worrell, B., Durinovic‐Belló, I., Cilio, C., Wong, F. S., & Schloot, N. C. (2010) · Clinical & Experimental Immunology · doi:10.1111/j.1365-2249.2010.04272.x · Cited by 261 · see citing works

Standardization of cytokine flow cytometry assays

Maecker, H. T., Rinfret, A., D’Souza, P., Darden, J. M., Roig, E., Landry, C., Hayes, P., Birungi, J., Anzala, O. A., Garcia, M., et al. (2005) · BMC Immunology · doi:10.1186/1471-2172-6-13 · Cited by 242 · see citing works

Myeloid-derived suppressor cell measurements in fresh and cryopreserved blood samples

Kotsakis, A. P., Harasymczuk, M., Schilling, B., Georgoulias, V., Argiris, A. E., & Whiteside, T. L. (2012) · Journal of Immunological Methods · doi:10.1016/j.jim.2012.04.004 · Cited by 199 · see citing works

Optimization and Limitations of Use of Cryopreserved Peripheral Blood Mononuclear Cells for Functional and Phenotypic T-Cell Characterization

Weinberg, A., Song, L., Wilkening, C. L., Sevin, A. D., Blais, B., Louzao, R., Stein, D., Defechereux, P. A., Durand, D., Riedel, E., et al. (2009) · Clinical and Vaccine Immunology · doi:10.1128/cvi.00342-08 · Cited by 194 · see citing works

Validation of CyTOF Against Flow Cytometry for Immunological Studies and Monitoring of Human Cancer Clinical Trials

Gadalla, R., Noamani, B., MacLeod, B. L., Dickson, R. J., Guo, M., Xu, W., Lukhele, S., Elsaesser, H. J., Razak, A. R. A., Hirano, N., et al. (2019) · Frontiers in Oncology · doi:10.3389/fonc.2019.00415 · Cited by 159 · see citing works

Maximizing the retention of antigen specific lymphocyte function after cryopreservation

Disis, M. L., Rosa, C. D., Vivian Goodell, A., Kuan, L.-Y., Chang, J. C. C., Kuus-Reichel, K., Clay, T. M., Lyerly, H. K., Bhatia, S., Ghanekar, S., et al. (2005) · Journal of Immunological Methods · doi:10.1016/j.jim.2005.09.011 · Cited by 153 · see citing works

Peripheral Blood Mononuclear Cells: Isolation, Freezing, Thawing, and Culture

Riedhammer, C., Halbritter, D., & Weissert, R. (2014) · Methods in molecular biology · doi:10.1007/7651_2014_99 · Cited by 148 · see citing works

Progressive contraction of the latent HIV reservoir around a core of less-differentiated CD4+ memory T Cells

Jaafoura, S., de Herve, M. G. D. G., Hernandez-Vargas, E. A., Hendel, H., Abdoh, M., Mateo, M. C., Krzysiek, R., Merad-Boudia, M., Seng, R., Tardieu, M., et al. (2014) · Nature Communications · doi:10.1038/ncomms6407 · Cited by 131 · see citing works

Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT

Maecker, H. T., Moon, J., Bhatia, S., Ghanekar, S., Maino, V. C., Payne, J. K., Kuus-Reichel, K., Chang, J. C. C., Summers, A. N., Clay, T. M., et al. (2005) · BMC Immunology · doi:10.1186/1471-2172-6-17 · Cited by 119 · see citing works

Different contribution of EBV and CMV infections in very long-term carriers to age-related alterations of CD8+ T cells

Vescovini, R., Telera, A. R., Fagnoni, F. F., Biasini, C., Medici, M. C., Valcavi, P., Pede, P. D., Lucchini, G., Zanlari, L., Passeri, G., et al. (2004) · Experimental Gerontology · doi:10.1016/j.exger.2004.04.004 · Cited by 119 · see citing works

Flow cytometric detection of endothelial microparticles (EMP): Effects of centrifugation and storage alter with the phenotype studied

van Ierssel, S. H., Craenenbroeck, E. M. V., Conraads, V. M., Tendeloo, V. F. V., Vrints, C. J., Jorens, P. G., & Hoymans, V. Y. (2010) · Thrombosis Research · doi:10.1016/j.thromres.2009.12.019 · Cited by 118 · see citing works

Loss of T cell responses following long-term cryopreservation

Owen, R. E., Sinclair, E., Emu, B., Heitman, J. W., Hirschkorn, D. F., Epling, C. L., Tan, Q. X., Custer, B., Harris, J. M., Jacobson, M. Z., et al. (2007) · Journal of Immunological Methods · doi:10.1016/j.jim.2007.07.012 · Cited by 103 · see citing works

Immune cell contexture in the bone marrow tumor microenvironment impacts therapy response in CML

Brück, O. E., Blom, S., Dufva, O., Turkki, R., Chheda, H., Ribeiro, A., Kovanen, P. E., Aittokallio, T. A., Koskenvesa, P., Kallioniemi, O., et al. (2018) · Leukemia · doi:10.1038/s41375-018-0175-0 · Cited by 102 · see citing works

Prebiotic‐supplemented partially hydrolysed cow's milk formula for the prevention of eczema in high‐risk infants: a randomized controlled trial

Boyle, R. J., Tang, M. L. K., Chiang, W. C., Chua, M. C., Ismail, I. H., Nauta, A. J., Hourihane, J. O., Smith, P. K., Gold, M., Ziegler, J. B., et al. (2016) · Allergy · doi:10.1111/all.12848 · Cited by 99 · see citing works

TH1 and TH2 cell polarization increases with aging and is modulated by zinc supplementation

Uciechowski, P., Kahmann, L., Plümäkers, B., Malavolta, M., Mocchegiani, E., Dedoussis, G., Herbein, G., Jajte, J., Fulop, T., & Rink, L. (2008) · Experimental Gerontology · doi:10.1016/j.exger.2007.11.006 · Cited by 97 · see citing works

Temperature fluctuations during deep temperature cryopreservation reduce PBMC recovery, viability and T-cell function

Germann, A., Oh, Y.-J., Schmidt, T., Schön, U., Zimmermann, H., & von Briesen, H. (2013) · Cryobiology · doi:10.1016/j.cryobiol.2013.06.012 · Cited by 95 · see citing works

The role of CCR5/CXCR3 expressing CD8+ cells in liver damage and viral control during persistent hepatitis C virus infection

Larrubia, J. R., Calvino, M., Martínez, S. B., Sanz‐de‐Villalobos, E., Perna, C., Pérez-Hornedo, J., González-Mateos, F., García-Garzón, S., Bienvenido, A., & Parra, T. (2007) · Journal of Hepatology · doi:10.1016/j.jhep.2007.04.009 · Cited by 89 · see citing works

VACUTAINER CPT and Ficoll density gradient separation perform equivalently in maintaining the quality and function of PBMC from HIV seropositive blood samples.

Ruitenberg, J. J., Mulder, C. B., Maino, V. C., LANDAY, A. L., & Ghanekar, S. (2006) · BMC Immunology · doi:10.1186/1471-2172-7-11 · Cited by 84 · see citing works

Sources of heterogeneity in human monocyte subsets

Appleby, L. J., Nausch, N., Midzi, N., Mduluza, T., Allen, J. E., & Mutapi, F. (2013) · Immunology Letters · doi:10.1016/j.imlet.2013.03.004 · Cited by 75 · see citing works

Panel Optimization for High‐Dimensional Immunophenotyping Assays Using Full‐Spectrum Flow Cytometry

Ferrer‐Font, L., Small, S. J., Lavender, B., Pilkington, K. R., Johnston, L. K., Park, L. M., Lannigan, J. A., Jaimes, M. C., & Price, K. M. (2021) · Current Protocols · doi:10.1002/cpz1.222 · Cited by 73 · see citing works

Optimizing recovery of frozen human peripheral blood mononuclear cells for flow cytometry

Hønge, B. L., Petersen, M. S., Olesen, R., Møller, B. K., & Erikstrup, C. (2017) · PLoS ONE · doi:10.1371/journal.pone.0187440 · Cited by 73 · see citing works

PLA2G2A+ cancer-associated fibroblasts mediate pancreatic cancer immune escape via impeding antitumor immune response of CD8+ cytotoxic T cells

Ge, W., Yue, M., Lin, R., Zhou, T., Xu, H., Wang, Y., Mao, T., Li, S., Wu, X., Zhang, X., et al. (2023) · Cancer Letters · doi:10.1016/j.canlet.2023.216095 · Cited by 72 · see citing works

Biospecimen Science of Blood for Peripheral Blood Mononuclear Cell (PBMC) Functional Applications

Betsou, F., Gaignaux, A., Ammerlaan, W., Norris, P. J., & Stone, M. (2019) · Current Pathobiology Reports · doi:10.1007/s40139-019-00192-8 · Cited by 69 · see citing works

Complementary Role of HCV and HIV in T-Cell Activation and Exhaustion in HIV/HCV Coinfection

Feuth, T., Arends, J. E., Fransen, J. H., Nanlohy, N. M., van Erpecum, K. J., Siersema, P. D., Hoepelman, A. I. M., & van Baarle, D. (2013) · PLoS ONE · doi:10.1371/journal.pone.0059302 · Cited by 68 · see citing works

Immune cell constitution in bone marrow microenvironment predicts outcome in adult ALL

Hohtari, H. R., Brück, O. E., Blom, S., Turkki, R., Sinisalo, M., Kovanen, P. E., Kallioniemi, O., Pellinen, T. S., Porkka, K., & Mustjoki, S. M. (2019) · Leukemia · doi:10.1038/s41375-018-0360-1 · Cited by 66 · see citing works

Effects of cryopreservation on effector cells for antibody dependent cell-mediated cytotoxicity (ADCC) and natural killer (NK) cell activity in 51Cr-release and CD107a assays

Mata, M. M., Mahmood, F., Sowell, R., & Baum, L. L. (2014) · Journal of Immunological Methods · doi:10.1016/j.jim.2014.01.017 · Cited by 64 · see citing works

Cryopreservation modulates the detection of regulatory T cell markers

Sattui, S. E., de la Flor, C., Sánchez, C., Lewis, D. E., López, G., Rizo‐Patrón, E., White, A. C., & Montes, M. (2011) · Cytometry Part B Clinical Cytometry · doi:10.1002/cyto.b.20621 · Cited by 63 · see citing works

A novel method for evaluating antibody-dependent cell-mediated cytotoxicity by flowcytometry using cryopreserved human peripheral blood mononuclear cells

Yamashita, M., Kitano, S., Aikawa, H., Kuchiba, A., Hayashi, M., Yamamoto, N., Tamura, K., & Hamada, A. (2016) · Scientific Reports · doi:10.1038/srep19772 · Cited by 63 · see citing works

Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation

Elkord, E. (2009) · Journal of Immunological Methods · doi:10.1016/j.jim.2009.06.001 · Cited by 61 · see citing works

A scoring system based on the expression of six surface molecules allows the identification of three prognostic risk groups in B‐cell chronic lymphocytic leukemia

Zucchetto, A., Bomben, R., Bo, M. D., Sonego, P., Nanni, P., Rupolo, M., Bulian, P., Maso, L. D., Poeta, G. D., Principe, M. I. D., et al. (2005) · Journal of Cellular Physiology · doi:10.1002/jcp.20570 · Cited by 57 · see citing works

Long‐term peripheral immune cell profiling reveals further targets of oral cladribine in MS

Moser, T., Schwenker, K., Seiberl, M., Feige, J., Akgün, K., Haschke‐Becher, E., Ziemssen, T., & Sellner, J. (2020) · Annals of Clinical and Translational Neurology · doi:10.1002/acn3.51206 · Cited by 56 · see citing works

Comprehensive evaluation of the effects of long-term cryopreservation on peripheral blood mononuclear cells using flow cytometry

Li, B., Yang, C., Jia, G., Liu, Y., Wang, N., Yang, F., Su, R. J., Shang, Y., & Han, Y. (2022) · BMC Immunology · doi:10.1186/s12865-022-00505-4 · Cited by 55 · see citing works

Cryopreservation of NK and T Cells Without DMSO for Adoptive Cell-Based Immunotherapy

Yao, X., & Matosevic, S. (2021) · BioDrugs · doi:10.1007/s40259-021-00494-7 · Cited by 54 · see citing works

Effects of Blood Transportation on Human Peripheral Mononuclear Cell Yield, Phenotype and Function: Implications for Immune Cell Biobanking

Posevitz‐Fejfár, A., Posevitz, V., Groß, C. C., Bhatia, U., Frank, K., Schütte, V., Bar‐Or, A., Meuth, S. G., & Wiendl, H. (2014) · PLoS ONE · doi:10.1371/journal.pone.0115920 · Cited by 54 · see citing works

Standardization of cryopreserved peripheral blood mononuclear cells through a resting process for clinical immunomonitoring—Development of an algorithm

Wang, L., Hückelhoven, A. G., Hong, J., Jin, N., Mani, J., Chen, B., Schmitt, M. W., & Schmitt, A. (2016) · Cytometry Part A · doi:10.1002/cyto.a.22813 · Cited by 54 · see citing works

Effect of delayed cell processing and cryopreservation on immunophenotyping in multicenter population studies

Thyagarajan, B., Barcelo, H., Crimmins, E. M., Weir, D. R., Minnerath, S., Vivek, S., & Faul, J. D. (2018) · Journal of Immunological Methods · doi:10.1016/j.jim.2018.09.007 · Cited by 54 · see citing works

Functional comparison of PBMCs isolated by Cell Preparation Tubes (CPT) vs. Lymphoprep Tubes

Chen, H. M., Schürch, C. M., Noble, K., Kim, K., Krutzik, P. O., O'Donnell, E. A., Tuig, J. V., Nolan, G. P., & McIlwain, D. R. (2020) · BMC Immunology · doi:10.1186/s12865-020-00345-0 · Cited by 50 · see citing works

Challenges in cryopreservation of regulatory T cells (Tregs) for clinical therapeutic applications

Gołąb, K., Leveson-Gower, D. B., Wang, X.-J., Grzanka, J., Marek-Trzonkowska, N. M., Krzystyniak, A., Millis, J. M., Trzonkowski, P., & Witkowski, P. (2013) · International Immunopharmacology · doi:10.1016/j.intimp.2013.02.001 · Cited by 52 · see citing works

A standardized immune phenotyping and automated data analysis platform for multicenter biomarker studies

Ivison, S., Malek, M., Garcia, R. V., Broady, R., Halpin, A., Richaud, M., Brant, R. F., Wang, S.-I., Goupil, M., Guan, Q., et al. (2018) · JCI Insight · doi:10.1172/jci.insight.121867 · Cited by 49 · see citing works

The novel tuberculosis vaccine, AERAS-402, is safe in healthy infants previously vaccinated with BCG, and induces dose-dependent CD4 and CD8T cell responses

Kagina, B. M. N., Tameris, M. D., Geldenhuys, H. D., Hatherill, M., Abel, B., Hussey, G. D., Scriba, T. J., Mahomed, H., SADOFF, J. C., Hanekom, W. A., et al. (2014) · Vaccine · doi:10.1016/j.vaccine.2014.09.001 · Cited by 49 · see citing works

Cryopreservation-related loss of antigen-specific IFNγ producing CD4+ T-cells can skew immunogenicity data in vaccine trials: Lessons from a malaria vaccine trial substudy

Ford, T., Wenden, C., Mbekeani, A. J., Dally, L., Cox, J. H., Morin, M. J., Winstone, N., Hill, A. V. S., Gilmour, J. W., & Ewer, K. (2017) · Vaccine · doi:10.1016/j.vaccine.2017.02.038 · Cited by 48 · see citing works

Assessment of the Phenotype and Functionality of Porcine CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus (CSFV) and Virulent CSFV Challenge

Franzoni, G., Kurkure, N. V., Edgar, D. S., Everett, H., Gerner, W., Bodman-Smith, K. B., Crooke, H., & Graham, S. P. (2013) · Clinical and Vaccine Immunology · doi:10.1128/cvi.00415-13 · Cited by 48 · see citing works

Toward Optimal Cryopreservation and Storage for Achievement of High Cell Recovery and Maintenance of Cell Viability and T Cell Functionality

Angel, S., von Briesen, H., Oh, Y.-J., Baller, M. K., Zimmermann, H., & Germann, A. (2016) · Biopreservation and Biobanking · doi:10.1089/bio.2016.0046 · Cited by 41 · see citing works

The impact of cryopreservation on human peripheral blood leucocyte bioenergetics

Keane, K. N., Calton, E. K., Cruzat, V. F., Soares, M. J., & Newsholme, P. (2015) · Clinical Science · doi:10.1042/cs20140725 · Cited by 43 · see citing works

Kinetics of human myeloid-derived suppressor cells after blood draw

Grützner, E. M., Stirner, R., Arenz, L., Athanasoulia, A. P., Schrödl, K., Berking, C., Bogner, J. R., & Draenert, R. (2016) · Journal of Translational Medicine · doi:10.1186/s12967-015-0755-y · Cited by 43 · see citing works

Lymph node biopsy analysis reveals an altered immunoregulatory balance already during the at‐risk phase of autoantibody positive rheumatoid arthritis

Ramwadhdoebé, T. H., Hähnlein, J. S., Maijer, K. I., van Boven, L. J., Gerlag, D. M., Tak, P. P., & van Baarsen, L. G. M. (2016) · European Journal of Immunology · doi:10.1002/eji.201646393 · Cited by 42 · see citing works

A Practical Cryopreservation and Staining Protocol for Immunophenotyping in Population Studies

Barcelo, H., Faul, J. D., Crimmins, E. M., & Thyagarajan, B. (2018) · Current Protocols in Cytometry · doi:10.1002/cpcy.35 · Cited by 42 · see citing works

Cryopreserved peripheral blood mononuclear cells are suitable for the assessment of immunological markers in type 1 diabetic children

Axelsson, S., Faresjö, M., Hedman, M., Ludvigsson, J., & Casas, R. (2008) · Cryobiology · doi:10.1016/j.cryobiol.2008.08.001 · Cited by 41 · see citing works

Expression of Chemokine Receptors on Intrahepatic and Peripheral Lymphocytes in Chronic Hepatitis C Infection: Its Relationship to Liver Inflammation

Wang, J., Holmes, T. H., Cheung, R. C., Greenberg, H. B., & He, X.-S. (2004) · The Journal of Infectious Diseases · doi:10.1086/423283 · Cited by 41 · see citing works

Standardized Serum-Free Cryomedia Maintain Peripheral Blood Mononuclear Cell Viability, Recovery, and Antigen-Specific T-Cell Response Compared to Fetal Calf Serum-Based Medium

Germann, A., Schulz, J. C., Kemp-Kamke, B., Zimmermann, H., & von Briesen, H. (2011) · Biopreservation and Biobanking · doi:10.1089/bio.2010.0033 · Cited by 40 · see citing works

Cryopreservation Decreases Receptor PD-1 and Ligand PD-L1 Coinhibitory Expression on Peripheral Blood Mononuclear Cell-Derived T Cells and Monocytes

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The Effect of Donor Graft Cryopreservation on Allogeneic Hematopoietic Cell Transplantation Outcomes: A Center for International Blood and Marrow Transplant Research Analysis. Implications during the COVID-19 Pandemic

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Cell banking for regulatory T cell-based therapy: strategies to overcome the impact of cryopreservation on the Treg viability and phenotype

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RNA-Stabilized Whole Blood Samples but Not Peripheral Blood Mononuclear Cells Can Be Stored for Prolonged Time Periods Prior to Transcriptome Analysis

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High dimensional flow cytometry for comprehensive leukocyte immunophenotyping (CLIP) in translational research

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Comparison of peripheral blood mononuclear cell isolation techniques and the impact of cryopreservation on human lymphocytes expressing CD39 and CD73

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Cryopreservation does not alter the frequency of regulatory T cells in peripheral blood mononuclear cells

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Pushing the frontiers of T-cell vaccines: accurate measurement of human T-cell responses

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Cell therapy products: focus on issues with manufacturing and quality control of chimeric antigen receptor T‐cell therapies

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Impact of Cryopreservation on Viability, Phenotype, and Functionality of Porcine PBMC

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Ex-Vivo Analysis of CD8+ T Cells Infiltrating Colorectal Tumors Identifies a Major Effector-Memory Subset with Low Perforin Content

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Heterogeneity of circulating CD8 T-cells specific to islet, neo-antigen and virus in patients with type 1 diabetes mellitus

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Fresh vs. frozen allogeneic peripheral blood stem cell grafts: A successful timely option

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Subsets of CD4+, CD8+, and CD25hi Lymphocytes Are in General Not Influenced by Isolation and Long-Term Cryopreservation

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Cryopreservation affected the levels of immune responses of PBMCs and antigen-presenting cells

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Standardization and performance evaluation of mononuclear cell cytokine secretion assays in a multicenter study

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Whole‐blood leukoreduction filters are a source for cryopreserved cells for phenotypic and functional investigations on peripheral blood lymphocytes

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Peripheral blood manipulation significantly affects the result of dendritic cell monitoring

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Value of a Quality Assessment Program in Optimizing Cryopreservation of Peripheral Blood Mononuclear Cells in a Multicenter Study

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CyTOF Measurement of Immunocompetence Across Major Immune Cell Types

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Cryopreservation of primary B cells minimally influences their signaling responses

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Effects of cryopreservation on CD4+ CD25+ T cells of HIV‐1 infected individuals

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Towards a xeno-free and fully chemically defined cryopreservation medium for maintaining viability, recovery, and antigen-specific functionality of PBMC during long-term storage

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Improvement of in vitro potency assays by a resting step for clinical-grade chimeric antigen receptor engineered T cells

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Humoral and Cellular Immune Responses to Measles and Tetanus: The Importance of Elapsed Time Since Last Exposure and the Nature of the Antigen

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Islet cell transplant and the incorporation of Tregs

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Granzyme B ELISPOT assay to measure influenza-specific cellular immunity

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Phenotyping of tumor infiltrating immune cells using mass-cytometry (CyTOF)

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Effects of long-term cryopreservation of PBMC on recovery of B cell subpopulations

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Generation, cryopreservation, function and in vivo persistence of ex vivo expanded cynomolgus monkey regulatory T cells

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Impacts of cryopreservation on phenotype and functionality of mononuclear cells in peripheral blood and ascites

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Quality Monitoring of HIV-1-Infected and Uninfected Peripheral Blood Mononuclear Cell Samples in a Resource-Limited Setting

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Thymic Function-Related Markers Within the Thymus and Peripheral Blood: Are They Comparable?

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Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot

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Single-cell analysis by mass cytometry reveals CD19 CAR T cell spatiotemporal plasticity in patients

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Surface-antigen expression profiling (SEP) in B-cell chronic lymphocytic leukemia (B-CLL): Identification of markers with prognostic relevance

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Effects of Cryopreservation on Canine Multipotent Stromal Cells from Subcutaneous and Infrapatellar Adipose Tissue

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Optimization of UC-MSCs cold-chain storage by minimizing temperature fluctuations using an automatic cryopreservation system

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Rapid expansion of T cells: Effects of culture and cryopreservation and importance of short-term cell recovery

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Guidelines for analysis of low-frequency antigen-specific T cell results: Dye-based proliferation assay vs 3H-thymidine incorporation

Blasi, D. D., Claessen, I., Turksma, A. W., van Beek, J., & ten Brinke, A. (2020) · Journal of Immunological Methods · doi:10.1016/j.jim.2020.112907 · Cited by 18 · see citing works

Viable Mononuclear Cell Stability Study for Implementation in a Proficiency Testing Program: Impact of Shipment Conditions

Kofanova, O. A., Davis, K., Glazer, B., Souza, Y. G. D., Kessler, J., Betsou, F., & Betsou, F. (2014) · Biopreservation and Biobanking · doi:10.1089/bio.2013.0090 · Cited by 18 · see citing works

Navigating the manufacturing, testing and regulatory complexities of regulatory T cells for adoptive cell therapy

Pikor, L. A., Arivazhagan, S., Mendicino, M., & Sathiamoorthy, S. (2025) · Frontiers in Immunology · doi:10.3389/fimmu.2025.1626085 · Cited by 17 · see citing works

Evaluation of T-activated proteins as recall antigens to monitor Epstein–Barr virus and human cytomegalovirus-specific T cells in a clinical trial setting

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2022 White Paper on Recent Issues in Bioanalysis: Enzyme Assay Validation, BAV for Primary End Points, Vaccine Functional Assays, Cytometry in Tissue, LBA in Rare Matrices, Complex NAb Assays, Spectral Cytometry, Endogenous Analytes, Extracellular Vesicles Part 2 – Recommendations on Biomarkers/CDx, Flow Cytometry, Ligand-Binding Assays Development & Validation; Emerging Technologies; Critical Reagents Deep Characterization

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DIFFERENTIAL MODULATION OF PHENOTYPIC COMPOSITION OF HIV-INFECTED AND -UNINFECTED PBMCS DURING CRYOPRESERVATION

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Preservation of Lymphocyte Immunophenotype and Proliferative Responses in Cryopreserved Peripheral Blood Mononuclear Cells from Human Immunodeficiency Virus Type 1-Infected Donors: Implications for Multicenter Clinical Trials

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Preservation of functionality, immunophenotype, and recovery of HIV RNA from PBMCs cryopreserved for more than 20 years

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Loss of T cell responses following long-term cryopreservation

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Effects of cryopreservation on CD4+ CD25+ T cells of HIV‐1 infected individuals

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