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Cited by 12Ion Transport and Channel RegulationIon channel regulation and functionOsteoarthritis Treatment and Mechanisms

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Physiological Effects of the Electrogenic Current Generated by the Na + /K + Pump in Mammalian Articular Chondrocytes

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Maleckar, Mary M., Martín-Vasallo, Pablo, Giles, Wayne R., Mobasheri, Ali·2020·Bioelectricity
DOI 10.1089/bioe.2020.0036PMC PMC8370340

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Abstract

Background: Although the chondrocyte is a nonexcitable cell, there is strong interest in gaining detailed knowledge of its ion pumps, channels, exchangers, and transporters. In combination, these transport mechanisms set the resting potential, regulate cell volume, and strongly modulate responses of the chondrocyte to endocrine agents and physicochemical alterations in the surrounding extracellular microenvironment. Materials and Methods: Mathematical modeling was used to assess the functional roles of energy-requiring active transport, the Na + /K + pump, in chondrocytes. Results: Our findings illustrate plausible physiological roles for the Na + /K + pump in regulating the resting membrane potential and suggest ways in which specific molecular components of pump can respond to the unique electrochemical environment of the chondrocyte. Conclusion: This analysis provides a basis for linking chondrocyte electrophysiology to metabolism and yields insights into novel ways of manipulating or regulating responsiveness to external stimuli both under baseline conditions and in chronic diseases such as osteoarthritis.

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References 96 · Cited by 12

Research works printed but not linked: 95

  1. 10.1016/j.berh.2011.11.013 · DOI
  2. 10.1111/j.1469-7580.2006.00546.x · DOI
  3. 10.1002/wsbm.157 · DOI
  4. 10.4137/CMAMD.S22147 · DOI
  5. 10.7554/eLife.16415 · DOI
  6. 10.1002/art.34520 · DOI
  7. 10.1016/j.joca.2011.01.003 · DOI
  8. 10.1016/S1357-2725(02)00301-1 · DOI
  9. Mobasheri A, , Mobasheri R, , Francis MJ, et al. Ion transport in chondrocytes: Membrane transporters involved in intracellular ion homeostasis and the regulation of cell volume, free [Ca2+] and pH. Histol Histopathol, 1998; 13:893–910. DOI: 10.14670/HH-13.893 · DOI
  10. 10.1006/cbir.2001.0826 · DOI
  11. 10.1177/0006355X2002039001002007 · DOI
  12. 10.1002/jcp.22075 · DOI
  13. 10.3389/fphys.2010.00135 · DOI
  14. 10.1007/s11926-015-0521-4 · DOI
  15. 10.1016/j.jbspin.2018.01.012 · DOI
  16. 10.1002/jcb.10217 · DOI
  17. 10.1007/s11926-019-0837-6 · DOI
  18. 10.3390/ijms13044433 · DOI
  19. 10.1002/jcp.22646 · DOI
  20. 10.4161/chan.22340 · DOI
  21. 10.4161/chan.26071 · DOI
  22. 10.1111/j.1476-5381.2012.02185.x · DOI
  23. 10.1186/s12860-020-00288-9 · DOI
  24. 10.3389/fphys.2018.01661 · DOI
  25. 10.1007/s004419900082 · DOI
  26. 10.3389/fendo.2019.00756 · DOI
  27. 10.2106/00004623-198062050-00018 · DOI
  28. 10.1016/j.maturitas.2014.12.003 · DOI
  29. 10.1007/s007760100064 · DOI
  30. 10.1073/pnas.1414298111 · DOI
  31. 10.1006/excr.2000.4847 · DOI
  32. 10.1007/s00776-004-0789-0 · DOI
  33. 10.1248/bpb.b18-00152 · DOI
  34. 10.1038/nrrheum.2017.50 · DOI · Paper page
  35. 10.1016/j.tvjl.2007.08.013 · DOI
  36. 10.3389/fphys.2018.00974 · DOI
  37. 10.1006/cbir.1997.0137 · DOI
  38. 10.1016/S1357-2725(96)00150-1 · DOI
  39. Trujillo E, , Alvarez de la Rosa D, , Mobasheri A, et al. Sodium transport systems in human chondrocytes. I. Morphological and functional expression of the Na+,K(+)-ATPase alpha and beta subunit isoforms in healthy and arthritic chondrocytes. Histol Histopathol, 1999; 14:1011–1022. DOI: 10.14670/HH-14.1011 · DOI
  40. 10.3390/ijms13045019 · DOI
  41. Mobasheri A. Correlation between [Na+], [glycosaminoglycan] and Na+/K+ pump density in the extracellular matrix of bovine articular cartilage. Physiol Res, 1998; 47:47–52.
  42. Mobasheri A. Regulation of Na+, K+-ATPase density by the extracellular ionic and osmotic environment in bovine articular chondrocytes. Physiol Res, 1999; 48:509–512.
  43. 10.1023/A:1005580332144 · DOI
  44. Glynn IM. Annual review prize lecture. “All hands to the sodium pump.” J Physiol (Lond), 1993; 462:1–30. DOI: 10.1113/jphysiol.1993.sp019540 · DOI
  45. Lingrel JB, , Van Huysse J, , O'Brien W, et al. Structure-function studies of the Na,K-ATPase. Kidney Int Suppl, 1994; 44:S32–S39.
  46. 10.1186/1471-2474-9-46 · DOI
  47. 10.1016/j.arthro.2005.01.011 · DOI
  48. 10.1007/BF01466616 · DOI
  49. 10.1016/S0006-3495(99)77246-2 · DOI
  50. 10.1016/S0006-3495(99)77245-0 · DOI
  51. 10.1007/BF01945511 · DOI
  52. Mobasheri A. The effect of the extracellular environment on sodium pump density in cartilage [dissertation]. Oxford UK: The University of Oxford; 1996.
  53. 10.4161/chan.4.3.11629 · DOI
  54. 10.1113/jphysiol.2011.210757 · DOI
  55. 10.1113/jphysiol.2003.058883 · DOI
  56. Hille B. Ion Channels of Excitable Membranes 3rd Edition. Sunderland MA: Sinauer Assoc. Inc. 2001.
  57. 10.1038/323628a0 · DOI
  58. Hille B. Ionic channels in excitable membranes. Current problems and biophysical approaches. Biophys J, 1978; 22:283–294. DOI: 10.1016/S0006-3495(78)85489-7. · DOI
  59. Sterratt DC. Q10: The Effect of Temperature on Ion Channel Kinetics. In: Jaeger D Jung R eds. Encyclopedia of Computational Neuroscience. New York NY: Springer 2014. DOI: 10.1007/978-1-4614-7320-6_236-1. · DOI
  60. 10.1016/S0006-3495(02)75434-9 · DOI
  61. Trujillo E, , Alvarez de la Rosa D, , Mobasheri A, et al. Sodium transport systems in human chondrocytes. II. Expression of ENaC, Na+/K+/2Cl- cotransporter and Na+/H+ exchangers in healthy and arthritic chondrocytes. Histol Histopathol, 1999; 14:1023–1031. DOI: 10.14670/HH-14.1023 · DOI
  62. 10.1113/jphysiol.2014.282319 · DOI
  63. 10.1016/S0021-9258(19)75984-5 · DOI
  64. 10.1152/ajpcell.1998.275.4.C1067 · DOI
  65. 10.1177/0006355X2004041003004012 · DOI
  66. 10.1152/ajprenal.1998.275.5.F633 · DOI
  67. 10.1016/j.bpj.2020.06.006 · DOI
  68. 10.1016/0221-8747(84)90019-5 · DOI
  69. 10.1152/ajpcell.1996.270.5.C1300 · DOI
  70. Mobasheri A. Brefeldin A influences the cell surface abundance and intracellular pools of low and high ouabain affinity Na+, K(+)-ATPase alpha subunit isoforms in articular chondrocytes. Histol Histopathol, 1999; 14:427–438. DOI: 10.14670/HH-14.427 · DOI
  71. 10.1073/pnas.0931278100 · DOI
  72. 10.1016/j.semnephrol.2005.03.004 · DOI
  73. 10.1016/j.bpj.2015.09.015 · DOI
  74. 10.1016/j.bpj.2014.05.054 · DOI
  75. 10.3390/cells9071577 · DOI
  76. 10.1042/cs0900061 · DOI
  77. Walker LM Holm A Cooling L et al. Mechanical manipulation of bone and cartilage cells with “optical tweezers.” FEBS Lett 1999;459:39–42. DOI: 10.1016/s0014-5793(99)01169-2 · DOI
  78. 10.1016/j.ceca.2010.09.006 · DOI
  79. 10.1016/bs.ctm.2016.10.003 · DOI
  80. 10.1007/s11010-008-9927-x · DOI
  81. 10.3389/fendo.2012.00099 · DOI
  82. 10.1016/j.joca.2003.12.005 · DOI
  83. 10.1007/s00264-004-0618-2 · DOI
  84. 10.1186/ar1894 · DOI
  85. 10.1042/bj3210095 · DOI
  86. 10.1016/j.freeradbiomed.2012.10.539 · DOI
  87. 10.1113/jphysiol.2014.282517 · DOI
  88. 10.1074/jbc.M109.090225 · DOI
  89. 10.1097/BOR.0000000000000353 · DOI
  90. 10.1177/1759720X12467868 · DOI · Paper page
  91. 10.7554/eLife.19267 · DOI
  92. 10.1152/physrev.00020.2014 · DOI
  93. 10.1016/S1063-4584(97)80022-2 · DOI
  94. 10.1002/art.27313 · DOI
  95. 10.1159/000448711 · DOI

Metadata

Authors
Maleckar, M. M., Martín-Vasallo, P., Giles, W. R., & Mobasheri, A.
Affiliations
  • Simula Research Laboratory (NO)
  • UD of Biochemistry and Molecular Biology, Universidad de La Laguna, San Cristóbal de La Laguna, Spain.
  • University of Calgary (CA)
  • University Medical Center Utrecht (NL)
Corresponding author
Maleckar, Mary M.
ORCID
Venue
Bioelectricity
Publisher
SAGE Publications
Published
2020-09-01
Volume
2
Issue
3
Pages
258-268
ISSN
  • 2576-3105 (ISSN-L)
  • 2576-3113
DOI
10.1089/bioe.2020.0036
PMC
PMC8370340
OpenAlex
W3100583014
Cited by
12
References
96
Citations by year
2024: 2 · 2023: 4 · 2022: 3 · 2021: 3
FWCI
0.81
Type
journal article
Language
en
Open access
Licence
Locations
  • Bioelectricity (publishedVersion, cc-by-nc)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
  • European Federation of Pharmaceutical Industries and Associations
  • European Commission (115770)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
Keywords
Na+/K+-ATPase, articular chondrocytes, resting membrane potential, ion transport, mathematical modeling, cell volume regulation, osteoarthritis, extracellular microenvironment

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