The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
The Expression of Mitochondrial DNA Transcription Factors during Early Cardiomyocyte In Vitro Differentiation from Human Embryonic Stem Cells
Abstract
Mitochondrial biogenesis and activation of both oxidative phosphorylation, as well as transcription and replication of the mitochondrial genome, are key regulatory events in cell differentiation. Mitochondrial DNA transcription and replication are highly dependent on the interaction with nuclear-encoded transcription factors translocated from the nucleus. Using a human embryonic stem cell line, HSF 6, we analyzed the proliferation of mitochondria and the expression of mtDNA-specific transcription factors in undifferentiated, migratory embryonic stem cells and spontaneously derived cardiomyocytes. Mitochondrial proliferation and mtDNA transcription are initiated in human embryonic stem cells as they undergo spontaneous differentiation in culture into beating cardiomyocytes. Undifferentiated, pluripotent human embryonic stem cells have few mitochondria, and, as they differentiate, they polarize to one extremity of the cell and then bipolarize the differentiating cell. The differentiated cell then adopts the cytoplasmic configuration of a somatic cell as evidenced in differentiating cardiomyocytes. Transcription and replication of the extranuclear mitochondrial genome is dependent on nuclear encoded factors exported to the mitochondrion. However, the differentiating cardiomyocytes have reduced or absent levels of these transcription and replication factors, namely mitochondrial transcription factors A, B1, B2, and nuclear respiratory factor 1 and polymerase gamma. Therefore, final embryonic stem cell commitment may be influenced by mitochondrial proliferation and mtDNA transcription. However, it is likely that differentiating cardiomyocytes are in mitochondrial arrest, awaiting commitment to a final cell fate.
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Cites
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Birky, C. W. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.25.11331 · Cited by 969
Mitochondrial transcription factor A regulates mtDNA copy number in mammals
Ekstrand, M. I. (2004) · Human Molecular Genetics · doi:10.1093/hmg/ddh109 · Cited by 919
Virbasius, J. V., & Scarpulla, R. C. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.4.1309 · Cited by 819
Gleyzer, N., Vercauteren, K., & Scarpulla, R. C. (2005) · Molecular and Cellular Biology · doi:10.1128/mcb.25.4.1354-1366.2005 · Cited by 711
Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA
Falkenberg, M., Gaspari, M., Rantanen, A., Trifunovic, A., Larsson, N.-G., & Gustafsson, C. M. (2002) · Nature Genetics · doi:10.1038/ng909 · Cited by 598 · cited 1×
“Those cells at the on-set and tubular mitochondria (Sathananthan et al., 2002) and, in mouse ESCs, also harvested to TFAM other factors factor B1 ( (Falkenberg possess both al., 2003).”
Genetic modification of survival in tissue-specific knockout mice with mitochondrial cardiomyopathy
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Cited by
Metabolic requirements for the maintenance of self-renewing stem cells
Ito, K., & Suda, T. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3772 · Cited by 1,014
Folmes, C. D. L., Nelson, T. J., Martinez-Fernandez, A., Arrell, D. K., Lindor, J. Z., Dzeja, P. P., Ikeda, Y., Perez-Terzic, C., & Terzic, A. (2011) · Cell Metabolism · doi:10.1016/j.cmet.2011.06.011 · Cited by 985
Energy Metabolism in Human Pluripotent Stem Cells and Their Differentiated Counterparts
Varum, S., Rodrigues, A. S., Moura, M. B., Momcilovic, O., Easley, C. A., Ramalho-Santos, J., Van Houten, B., & Schatten, G. (2011) · PLoS ONE · doi:10.1371/journal.pone.0020914 · Cited by 671
Self-citationLopaschuk, G. D., & Jaswal, J. S. (2010) · Journal of Cardiovascular Pharmacology · doi:10.1097/fjc.0b013e3181e74a14 · Cited by 636
Ramalho-Santos, J., Varum, S., Amaral, S., Mota, P. C., Sousa, A. P., & Amaral, A. (2009) · Human Reproduction Update · doi:10.1093/humupd/dmp016 · Cited by 482
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Metadata
- Authors
- St. John, J. C., Ramalho-Santos, J., Gray, H. L., Petrosko, P., Rawe, V. Y., Navara, C. S., Simerly, C. R., & Schatten, G. P.
- Venue
- Cloning and Stem Cells
- Publisher
- SAGE Publications
- Published
- 2005-09
- Volume
- 7
- Issue
- 3
- Pages
- 141-153
- ISSN
- 1536-2302 (ISSN-L)
- 1557-7457
- OpenAlex
- W1980699340
- Cited by
- 231
- References
- 7
- FWCI
- 2.19
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: The Expression of Mitochondrial DNA Transcription Factors during Early Cardiomyocyte In Vitro Differentiation from Human Embryonic Stem Cells. https://app.cochat.ai/papers/10.1089/clo.2005.7.141
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- St. John, J. C., Ramalho-Santos, J., Gray, H. L., Petrosko, P., Rawe, V. Y., Navara, C. S., Simerly, C. R., & Schatten, G. P. (2005). The Expression of Mitochondrial DNA Transcription Factors during Early Cardiomyocyte In Vitro Differentiation from Human Embryonic Stem Cells. Cloning and Stem Cells. https://doi.org/10.1089/clo.2005.7.141
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