The DOI matches its Crossref record.
cDNA Sequence of Two Distinct Pituitary Proteins Homologous to Kex2 and Furin Gene Products: Tissue-Specific mRNAs Encoding Candidates for Pro-Hormone Processing Proteinases
Abstract
Based on the concept of sequence conservation around the active sites of serine proteinases, polymerase chain reaction applied to mRNA amplification allowed us to obtain a 260-bp probe which was used to screen a mouse pituitary cDNA library. The primers used derived from the cDNA sequence of active sites Ser* and Asn* of human furin. Two cDNA sequences were obtained from a number of positive clones. These code for two similar but distinct structures (mPC1 and mPC2), each being homologous to yeast Kex2 and human furin. In situ hybridization (mPC1) and Northern blots (mPC1 = 3.0 kb and mPC2 = 2.8 and 4.8 kb) demonstrated tissue and cellular specificity of expression, only within endocrine and neuroendocrine cells. These data suggest that mPC1 and mPC2 represent prime candidates for tissue-specific pro-hormone converting proteinases.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
The biology and therapeutic targeting of the proprotein convertases
Seidah, N. G., & Prat, A. (2012) · Nature Reviews Drug Discovery · doi:10.1038/nrd3699 · Cited by 759
Self-citationSeidah, N. G., Chrétien, M., & Day, R. (1994) · Biochimie · doi:10.1016/0300-9084(94)90147-3 · Cited by 396
Self-citationImplication of the proprotein convertase NARC-1/PCSK9 in the development of the nervous system
Poirier, S., Prat, A., Marcinkiewicz, E., Paquin, J., Chitramuthu, B. P., Baranowski, D., Cadieux, B., Bennett, H. P. J., & Seidah, N. G. (2006) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2006.03928.x · Cited by 121
Self-citationAnalyses of PCSK9 Post-translational Modifications Using Time-of-Flight Mass Spectrometry
Dewpura, T., & Mayne, J. (2011) · Methods in Molecular Biology · doi:10.1007/978-1-61779-204-5_8 · Cited by 8
Metadata
- Authors
- SEIDAH, N. G., GASPAR, L., MION, P., MARCINKIEWICZ, M., MBIKAY, M., & CHRÉTIEN, M.
- Venue
- DNA and Cell Biology
- Publisher
- SAGE Publications
- Published
- 1990-07
- Volume
- 9
- Issue
- 6
- Pages
- 415-424
- ISSN
- 1044-5498 (ISSN-L)
- 1557-7430
- OpenAlex
- W1975096426
- Cited by
- 512
- References
- 0
- FWCI
- 32.77
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: cDNA Sequence of Two Distinct Pituitary Proteins Homologous to Kex2 and Furin Gene Products: Tissue-Specific mRNAs Encoding Candidates for Pro-Hormone Processing Proteinases. https://app.cochat.ai/papers/10.1089/dna.1990.9.415
- The paper
- SEIDAH, N. G., GASPAR, L., MION, P., MARCINKIEWICZ, M., MBIKAY, M., & CHRÉTIEN, M. (1990). cDNA Sequence of Two Distinct Pituitary Proteins Homologous to Kex2 and Furin Gene Products: Tissue-Specific mRNAs Encoding Candidates for Pro-Hormone Processing Proteinases. DNA and Cell Biology. https://doi.org/10.1089/dna.1990.9.415
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
