Molecular Characterization of Staphylococcus aureus in the United States 2004–2008 Reveals the Rapid Expansion of USA300 Among Inpatients and Outpatients
Abstract
To assess the clonal structure of Staphylococcus aureus in the United States, we performed a molecular epidemiological study of 1,055 S. aureus isolates from a nationally representative clinical isolate collection from 2004–2008. Resistant and susceptible isolates were typed with multilocus sequence typing, tested for the presence of Panton-Valentine leukocidin (PVL), and serotyped. USA300 (multilocus sequence typing clonal complex 8, PVL positive, and methicillin-resistant) was the most frequently isolated clone, expanding from 12% of all isolates in 2004 to 38% in 2006. The USA300 clone increased significantly in frequency among both outpatients and inpatients. USA300 increased in both skin and soft-tissue and invasive infection isolates. The second most frequently observed clone was clonal complex 5, PVL-negative, and methicillin-resistant, and its frequency was stable from 2004–2008. The methicillin-susceptible S. aureus in the study was polyclonal, and decreased in frequency as it was replaced by USA300.
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Increased incidence of MRSA-associated infections in both acute and chronic wounds is well-documented.
“Increased incidence of MRSA-associated infections in both acute and chronic wounds is well-documented [3], [21], [22]”
From Interactions of Methicillin Resistant Staphylococcus aureus USA300 and Pseudomonas aeruginosa in Polymicrobial Wound Infection (2013) · cites this paper as [22]
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- Authors
- O'Hara, F. P., Amrine-Madsen, H., Mera, R. M., Brown, M. L., Close, N. M., Suaya, J. A., & Acosta, C. J.
- Affiliations
- Department of Computational Biology, GlaxoSmithKline, Collegeville, Pennsylvania.
- Head of Assay Development, DBU, GlaxoSmithKline, Research Triangle Park, North Carolina.
- GlaxoSmithKline (United States) (US)
- Venue
- Microbial Drug Resistance
- Publisher
- SAGE Publications
- Published
- 2012-12
- Volume
- 18
- Issue
- 6
- Pages
- 555-561
- ISSN
- 1076-6294 (ISSN-L)
- 1931-8448
- OpenAlex
- W1991180141
- Cited by
- 45
- References
- 29
- Citations by year
- 2026: 1 · 2023: 1 · 2022: 2 · 2021: 6 · 2020: 5 · 2019: 3 · 2018: 4 · 2016: 4 · 2015: 3 · 2014: 6 · 2013: 8 · 2012: 1
- FWCI
- 3.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Microbial Drug Resistance (publishedVersion)
- PubMed (publishedVersion)
- Funders
- GlaxoSmithKline
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antibiotic Use and Resistance
- Keywords
- Staphylococcus aureus, USA300, clonal expansion, clonal complex 8, clonal complex 5, Panton-Valentine leukocidin, methicillin resistance, methicillin-susceptible Staphylococcus aureus, molecular epidemiology, skin and soft tissue infections, invasive infections, inpatients and outpatients, serotyping, antimicrobial resistance
- MeSH terms
- Adolescent, Adult, Aged, Aged, 80 and over, Anti-Bacterial Agents, Bacterial Toxins, Child, Child, Preschool, Clone Cells, Exotoxins, Female, Humans, Infant, Infant, Newborn, Inpatients, Leukocidins, Male, Middle Aged, Outpatients, Retrospective Studies, Serotyping, Skin, Staphylococcal Infections, Staphylococcal Skin Infections, Staphylococcus aureus, United States, Incidence, Methicillin Resistance, Soft Tissue Infections, Multilocus Sequence Typing
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