Characterizing White Matter Damage in Rat Spinal Cord with Quantitative MRI and Histology
Abstract
Diffusion tensor imaging (DTI) and quantitative T(2) magnetic resonance imaging (MRI) were used to characterize ex vivo the white matter damage at 3 and 8 weeks following dorsal column transection (DC Tx) injury at the cervical level C5 of rat spinal cords. Luxol Fast Blue (LFB) and myelin basic protein (MBP) staining was used to assess myelin damage, and neurofilament-H in combination with neuron specific beta-III-tubulin (NF/Tub) staining was used to assess axonal damage. Average values of myelin water fraction (MWF), fractional anisotropy (FA), longitudinal diffusivity (D(long)), transverse diffusivity (D(trans)), and average diffusivity (D(ave)) were calculated in the fasciculus gracilis, fasciculus cuneatus, and the dorsal corticospinal tract (CST) 5 mm cranial, as well as 5 and 10 mm caudal to injury and correlated with histology. These tracts were selected as these contain bundles of parallel ascending and descending axons in very circumscribed areas with little intermingling of other axonal populations. Axonal and myelin degeneration occur cranial to injury in the funiculus gracilis and caudal to injury in the CST. Both MWF and D(trans) showed significant correlation with LFB staining at 3 weeks (0.64 and -0.49, respectively) and 8 weeks post-injury (0.88 and -0.71, respectively). Both D(long) and FA correlated significantly with NF/Tub staining at 3 weeks post-injury (0.78 and 0.64, respectively), while only D(long) displayed significant correlation 8 weeks post-inju…
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In papers that used multiple histological methods, validation results were not always the same for different histological markers.
“In the few papers that used multiple histological methods, validation results were not always the same for the different histological markers used”
From Can MRI measure myelin? Systematic review, qualitative assessment, and meta-analysis of studies validating microstructural imaging with myelin histology (2021) · cites this paper as Kozlowski, Raj, Liu, Lam, Yung, Tetzlaff, 2008
Cites
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Myelin Water Fraction in Human Cervical Spinal Cord In Vivo
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Lazari, A., & Lipp, I. (2021) · NeuroImage · doi:10.1016/j.neuroimage.2021.117744 · Cited by 176 · see citing works
“In the few papers that used multiple histological methods, validation results were not always the same for the different histological markers used (e.g. Kozlowski et al. (2008); Laule et al. (2011)).”
Method
- Authors
- Kozlowski, P., Raj, D., Liu, J., Lam, C., Yung, A. C., & Tetzlaff, W.
- Affiliations
- Departments of Radiology and Urologic Sciences, University of British Columbia, Vancouver, Canada.
- University of British Columbia (CA)
- International Collaboration On Repair Discoveries (CA)
- Venue
- Journal of Neurotrauma
- Publisher
- SAGE Publications
- Published
- 2008-06
- Volume
- 25
- Issue
- 6
- Pages
- 653-676
- ISSN
- 0897-7151 (ISSN-L)
- 1557-9042
- OpenAlex
- W2082419792
- Cited by
- 129
- References
- 4
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 2 · 2023: 5 · 2022: 6 · 2021: 10 · 2020: 11 · 2019: 7 · 2018: 10 · 2017: 7 · 2016: 7 · 2015: 9 · 2014: 6 · 2013: 12 · 2012: 9
- FWCI
- 4.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neurotrauma (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Health Canada
- Canadian Institutes of Health Research
- Natural Sciences and Engineering Research Council of Canada
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced Neuroimaging Techniques and Applications
- Health Sciences › Medicine › Pathology and Forensic Medicine › Spinal Cord Injury Research
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Keywords
- spinal cord injury, white matter injury, myelin degeneration, axonal degeneration, myelin water fraction, fractional anisotropy, diffusion tensor imaging, neurofilament H, Luxol fast blue, myelin basic protein, fasciculus gracilis, rat model
- MeSH terms
- Animals, Body Water, Diffusion, Disease Models, Animal, Myelin Basic Protein, Immunohistochemistry, Male, Nerve Fibers, Myelinated, Neural Pathways, Predictive Value of Tests, Spinal Cord, Spinal Cord Injuries, Staining and Labeling, Time Factors, Tubulin, Wallerian Degeneration, Biomarkers, Trauma Severity Indices, Anisotropy, Neurofilament Proteins, Rats, Sprague-Dawley, Disease Progression, Diffusion Magnetic Resonance Imaging, Rats
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