Angiotensin II-Induced Erythrocyte Senescence Contributes to Oxidative Stress
Abstract
Oxidative stress may be an important cause of erythrocyte senescence. Angiotensin II (Ang II) has recently been shown to promote vascular cell senescence. However, its effects on erythrocytes remain unclear. This study aims at investigating the role of Ang II in regulating erythrocyte lifespan through oxidative stress. Experiments were performed in C57/BL6J mice infused with Ang II (1500 ng/kg per minute) or saline for 7 days. After Ang II infusion, we found that Ang II increased erythrocyte number, hemoglobin, and red blood cell distribution width. These differences were accompanied by a decrease in glutathione (GSH) and an increase in malondialdehyde (MDA) concentration. In vitro , after 24 hours of Ang II treatment, erythrocytes showed reduced surface expression of CD47 and increased phosphatidylserine exposure. In parallel, Ang II reduced the levels of antioxidant enzymes, including Cu/ZnSOD, catalase, and peroxidase 2 (PRDX2). These effects were reversed by the addition of the antioxidant N-acetyl-L-cysteine or the Ang II type 1 (AT1) receptor blocker losartan. In addition, Ang II treatment increased pro-inflammatory oxylipin, including hydroxyeicosatetraenoic acids (HETEs) and dihydroxyoctadecenoic acids (DiHOMEs), in the erythrocyte membranes. Collectively, Ang II induced erythrocyte senescence and susceptibility to eryptosis, partially due to enhanced oxidative stress.
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Normal aging and cellular senescence are conditions influenced by oxidative stress.
“both conditions influenced by OS [183,184,185,186].”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [186]
A reduction in GSH and the GSH/GSSG ratio is often reported during normal aging and in cellular senescence.
“a reduction in GSH and the GSH/GSSG ratio are often reported during normal aging and in cellular senescence, both conditions influenced by OS [183,184,185,186].”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [186]
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- Authors
- Huang, C., Gao, J., Wei, T., & Shen, W.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Corresponding author
- Shen, Weili
- Venue
- Rejuvenation Research
- Publisher
- SAGE Publications
- Published
- 2022-02
- Volume
- 25
- Issue
- 1
- Pages
- 30-38
- ISSN
- 1094-5458 (ISSN-L)
- 1549-1684
- 1557-8577
- OpenAlex
- W4205395647
- Cited by
- 15
- References
- 38
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 5 · 2023: 2 · 2022: 3
- FWCI
- 1.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Rejuvenation Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Erythrocyte Function and Pathophysiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- angiotensin II, erythrocyte senescence, oxidative stress, eryptosis, erythrocyte lifespan, CD47, phosphatidylserine exposure, antioxidant enzymes, N-acetylcysteine, losartan, angiotensin II type 1 receptor, oxylipins, hydroxyeicosatetraenoic acids, glutathione, malondialdehyde, erythrocyte membrane
- MeSH terms
- Angiotensin II, Animals, Erythrocytes, Cellular Senescence, Oxidative Stress, Losartan, Mice
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