Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 13

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Promoting Maturation of Human Pluripotent Stem Cell-Derived Renal Microtissue by Incorporation of Endothelial and Mesenchymal Cells

Khoshdel-Rad, Niloofar, Zahmatkesh, Ensieh, Moeinvaziri, Farideh, Haghparast, Newsha, Baharvand, Hossein, Aghdami, Nasser, Moghadasali, Reza·2021·Stem Cells and Development
DOI 10.1089/scd.2020.0189
Open this paper in CoChat — free account

Abstract

Directed differentiation of human pluripotent stem cells (hPSCs) uses a growing number of small molecules and growth factors required for in vitro generation of renal lineage cells. Although current protocols are relatively inefficient or expensive. The first objective of the present work was to establish a new differentiation protocol for generating renal precursors. We sought to determine if inducer of definitive endoderm 1 (IDE1), a cost-effective small molecule, can be used to replace activin A. Gene expression data showed significantly increased expressions of nephrogenic markers in cells differentiated with 20 nM IDE1 compared with cells differentiated with activin A. Thus, renal lineage cells could be generated by this alternative approach. Afterward, we determined whether coculture of endothelial and mesenchymal cells could increase the maturation of three-dimensional (3D) renal structures. For this purpose, we employed a 3D coculture system in which hPSC-derived kidney precursors were cocultured with endothelial cells (ECs) and mesenchymal stem cells (MSCs), hereafter named RMEM (renal microtissue derived from coculture of renal precursors with endothelial and mesenchymal stem cells). hPSC-derived kidney precursors were cultured either alone [renal microtissue (RM)] or in coculture with human umbilical vein endothelial cells and human bone marrow-derived mesenchymal stem cells at an approximate ratio of 10:7:2, respectively. Immunofluorescent staining showed expressi…

Overview

Integrity signals

Registry

Crossref · 10.1089/scd.2020.0189

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 3 references

Research works printed but not linked: 38

  1. 10.2174/1574888X15666191218094513 · DOI
  2. 10.3389/fcell.2020.00183 · DOI
  3. 10.1038/nbt.3392 · DOI
  4. 10.1016/j.stemcr.2018.01.041 · DOI
  5. 10.1038/s41592-019-0325-y · DOI · Paper page
  6. 10.1038/s41563-019-0287-6 · DOI
  7. Takebe T, 2015, Stem Cell, 16, 556
  8. 10.1016/j.stem.2017.10.011 · DOI
  9. 10.1242/dev.093740 · DOI
  10. 10.1242/dev.035592 · DOI
  11. 10.1016/j.ydbio.2009.02.034 · DOI
  12. 10.1098/rstb.2011.0006 · DOI
  13. 10.1016/j.ydbio.2006.07.026 · DOI
  14. Bronner-Fraser M, 1988, Nature, 336, 403, 10.1038/336403a0 · DOI
  15. 10.1038/s41598-017-03808-4 · DOI
  16. 10.1016/j.stem.2018.10.010 · DOI
  17. 10.1242/dev.142794 · DOI
  18. 10.1089/ten.tea.2010.0397 · DOI
  19. 10.1016/j.yjmcc.2013.09.005 · DOI
  20. 10.1038/s12276-019-0336-x · DOI
  21. Zaret KS, 2009, Stem Cell, 4, 373
  22. 10.1016/j.stem.2009.01.014 · DOI
  23. 10.1002/jbm.a.35039 · DOI
  24. 10.1002/jcp.27644 · DOI
  25. 10.1002/cpsc.49 · DOI
  26. 10.1016/j.celrep.2017.11.005 · DOI
  27. 10.1016/j.biomaterials.2018.11.033 · DOI
  28. Yokoo T, 2015, J Am Soc Nephrol, 27, 1400
  29. 10.1016/j.ebiom.2018.08.014 · DOI
  30. Holloway EM, 2019, Development, 146
  31. 10.1016/j.jcyt.2014.01.004 · DOI
  32. 10.1016/j.jcyt.2018.02.368 · DOI
  33. 10.1186/s13287-017-0557-7 · DOI
  34. 10.1002/jcp.28094 · DOI
  35. 10.3390/ijms20205238 · DOI · Paper page
  36. Randjelović P, 2017, EXCLI J, 16, 388
  37. 10.1038/nprot.2014.102 · DOI · Paper page
  38. 10.1089/scd.2016.0244 · DOI

Cited by

See all 13

Metadata

Authors
Khoshdel-Rad, N., Zahmatkesh, E., Moeinvaziri, F., Haghparast, N., Baharvand, H., Aghdami, N., & Moghadasali, R.
Affiliations
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Venue
Stem Cells and Development
Publisher
SAGE Publications
Published
2021-04-15
Volume
30
Issue
8
Pages
428-440
ISSN
  • 1547-3287
  • 1557-8534
DOI
10.1089/scd.2020.0189
Cited by
13
References
3
Language
en
Licence
www.liebertpub.com/nv/resources-tools/text-and-data-mining-policy/121/ (tdm)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Promoting Maturation of Human Pluripotent Stem Cell-Derived Renal Microtissue by Incorporation of Endothelial and Mesenchymal Cells. https://app.cochat.ai/papers/10.1089/scd.2020.0189
The paper
Khoshdel-Rad, N., Zahmatkesh, E., Moeinvaziri, F., Haghparast, N., Baharvand, H., Aghdami, N., & Moghadasali, R. (2021). Promoting Maturation of Human Pluripotent Stem Cell-Derived Renal Microtissue by Incorporation of Endothelial and Mesenchymal Cells. Stem Cells and Development. https://doi.org/10.1089/scd.2020.0189