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Three-Dimensional Cell Culture Matrices: State of the Art
Open accessAbstract
Traditional methods of cell growth and manipulation on 2-dimensional (2D) surfaces have been shown to be insufficient for new challenges of cell biology and biochemistry, as well as in pharmaceutical assays. Advances in materials chemistry, materials fabrication and processing technologies, and developmental biology have led to the design of 3D cell culture matrices that better represent the geometry, chemistry, and signaling environment of natural extracellular matrix. In this review, we present the status of state-of-the-art 3D cell-growth techniques and scaffolds and analyze them from the perspective of materials properties, manufacturing, and functionality. Particular emphasis was placed on tissue engineering and in vitro modeling of human organs, where we see exceptionally strong potential for 3D scaffolds and cell-growth methods. We also outline key challenges in this field and most likely directions for future development of 3D cell culture over the period of 5-10 years.
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“Cellular responses to drug treatments in 3D cultures have been shown to be more similar to what occurs in vivo compared to 2D culture. 11-13”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [13]
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“It has been demonstrated that cell responses in 3D cultures are more similar to in vivo behavior compared to 2D culture. 11-13”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [13]
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“In both cases, the data agreed with values obtained from 2D/3D cell cultures in 96-well plates or from the literature, demonstrating that the 3D array's miniaturization has no appreciable effect on either cell line's cytotoxicity responses. 13”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [13]
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Heller, C. M., Schwentenwein, M., Porodec, M., Schulz‐Siegmund, M., Stampfl, J., Varga, F., & Liska, R. (2009) · World Congress on Medical Physics and Biomedical Engineering, September 7 - 12, 2009, Munich, Germany · doi:10.1007/978-3-642-03900-3_88 · Cited by 1
Stem Cell Response to Biomaterial Topography
Nguyen, L. T., Liao, S., Chan, C. K., & Ramakrishna, S. (2012) · CRC Press eBooks · doi:10.1201/b12083-15 · Cited by 1
Introduction to Bio-Inspired Hydrogel and Their Application
Melnyczuk, J. M., & Palchoudhury, S. (2016) · Advances in chemical and materials engineering book series · doi:10.4018/978-1-4666-9811-6.ch005 · Cited by 1
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Ntamo, Y., Samodien, E., Muller, C. J. F., Louw, J., & Chellan, N. (2020) · Tissue and Cell · doi:10.1016/j.tice.2020.101367 · Cited by 1
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Metadata
- Authors
- Lee, J., Cuddihy, M. J., & Kotov, N. A.
- Affiliations
- University of Michigan (US)
- Venue
- Tissue Engineering Part B: Reviews
- Publisher
- SAGE Publications
- Published
- 2008-03
- Volume
- 14
- Issue
- 1
- Pages
- 61-86
- ISSN
- 1937-3368 (ISSN-L)
- 1937-3376
- OpenAlex
- W2043156676
- Cited by
- 1,047
- References
- 209
- Citations by year
- 2026: 20 · 2025: 37 · 2024: 32 · 2023: 51 · 2022: 54 · 2021: 63 · 2020: 66 · 2019: 60 · 2018: 68 · 2017: 66 · 2016: 81 · 2015: 66 · 2014: 72 · 2013: 58 · 2012: 77
- FWCI
- 38.55
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Tissue Engineering Part B Reviews (publishedVersion)
- PubMed (publishedVersion)
- Deep Blue (University of Michigan) (submittedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Keywords
- 3D cell culture, cell culture scaffolds, extracellular matrix mimics, tissue engineering, in vitro organ models, scaffold fabrication, scaffold materials, pharmaceutical assay
- MeSH terms
- Animals, Biocompatible Materials, Biomedical Engineering, Culture Media, Humans, Materials Testing, Microscopy, Electron, Scanning, Surface Properties, Cell Culture Techniques, Imaging, Three-Dimensional, Tissue Engineering, Nanotechnology, Culture Techniques, Cell Proliferation
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