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Cited by 236Tissue Engineering and Regenerative Medicine3D Printing in Biomedical ResearchLiver physiology and pathology

The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.

Assessing Porcine Liver-Derived Biomatrix for Hepatic Tissue Engineering

Lin, Paul, Chan, Warren C.W., Badylak, Stephen F., Bhatia, Sangeeta N.·2004·Tissue Engineering
DOI 10.1089/ten.2004.10.1046
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Abstract

Acellular, biologically derived matrices such as small intestinal submucosa have been extensively utilized to induce tissue regeneration and remodeling of connective tissue, vascular grafts, and urinary bladder; however, decellularized scaffolds have not been explored for their potential utility in hepatic tissue engineering. In the case of both extracorporeal hepatocyte-based devices and implantable hepatocyte-scaffold tissue-engineered constructs, maintenance of hepatocellular function is of prime importance. In this study, we specifically explored decellularized, porcine, liver-derived biomatrix (LBM) as a bioresorbable scaffold for primary hepatocytes. Primary rat hepatocytes were cultured on LBM and compared with well-characterized hepatocyte culture models--double-gel cultures that promote maintenance of liver-specific functions for many weeks, and adsorbed collagen monolayers that lead to the rapid decline of hepatocellular function and viability. Hepatocytes were maintained for up to 45 days on LBM and liver-specific functions such as albumin synthesis, urea production, and P-450 IA1 activity were found to be significantly improved over adsorbed collagen cultures. Our data indicate that LBM may be a favorable alternative to existing scaffolds for tissue engineering in that it is bioresorbable, can be easily manipulated, and supports long-term hepatocellular functions in vitro.

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Crossref · 10.1089/ten.2004.10.1046

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Research works printed but not linked: 2

  1. Bissell D.M., 1986, Eur. J. Cell Biol., 40, 72
  2. Burke M.D., 1975, Drug Metab. Dispos., 3, 245

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Metadata

Authors
Lin, P., Chan, W. C. W., Badylak, S. F., & Bhatia, S. N.
Affiliations
  • University of California San Diego (US)
  • University of Toronto (CA)
  • University of Pittsburgh (US)
ORCID
Venue
Tissue Engineering
Publisher
SAGE Publications
Published
2004-07
Volume
10
Issue
7-8
Pages
1046-1053
ISSN
  • 1076-3279 (ISSN-L)
  • 1557-8690
DOI
10.1089/ten.2004.10.1046
OpenAlex
W2030077110
Cited by
236
References
52
Citations by year
2026: 1 · 2025: 4 · 2024: 11 · 2023: 10 · 2022: 11 · 2021: 14 · 2020: 12 · 2019: 15 · 2018: 9 · 2017: 8 · 2016: 10 · 2015: 17 · 2014: 20 · 2013: 17 · 2012: 11
FWCI
5.97
Type
journal article
Language
en
Open access
closed
Licence
www.liebertpub.com/nv/resources-tools/text-and-data-mining-policy/121/ (tdm)
Locations
  • Tissue Engineering (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Heart, Lung, and Blood Institute (T32HL007089)
  • National Institute of Diabetes and Digestive and Kidney Diseases (R01DK056966, R01DK065152)
  • National Institute of Biomedical Imaging and Bioengineering (R01EB000506)
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
Keywords
porcine liver, liver tissue engineering, bioresorbable scaffolds, primary hepatocytes, hepatocyte culture, albumin synthesis, urea synthesis, cytochrome P450
MeSH terms
Animals, Cell Adhesion, Cell Survival, Cells, Cultured, Extracellular Matrix, Female, Liver, Rats, Inbred Lew, Cell Culture Techniques, Liver, Artificial, Hepatocytes, Tissue Engineering, Cell Size, Rats

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