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Cited by 356Tissue Engineering and Regenerative MedicineWound Healing and TreatmentsElectrospun Nanofibers in Biomedical Applications

Macrophage Participation in the Degradation and Remodeling of Extracellular Matrix Scaffolds

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Valentin, Jolene E., Stewart-Akers, Ann M., Gilbert, Thomas W., Badylak, Stephen F.·2009·Tissue Engineering Part A
DOI 10.1089/ten.tea.2008.0419PMC PMC2792102
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Abstract

Biologic scaffolds composed of extracellular matrix (ECM) are widely used to facilitate remodeling and reconstruction of a variety of tissues in both preclinical animal studies and human clinical applications. The mechanisms by which such scaffolds influence the host tissue response are only partially understood, but it is logical that the mononuclear macrophage cell population plays a central role. The present study evaluated the role of macrophages that derive from peripheral blood in the degradation of ECM scaffolds. An established rat body wall reconstruction model was used to evaluate the degradation of carbodiimide (CDI)-crosslinked scaffolds composed of porcine small intestinal submucosa (SIS), noncrosslinked SIS, and autologous body wall. To assess the role of circulating macrophages in the degradation process, the degradation of each scaffold was assessed with and without macrophage depletion caused by administration of clodronate-containing liposomes. Results showed that peripheral blood monocytes are required for the early and rapid degradation of both SIS scaffolds and autologous body wall, and that CDI crosslinked SIS is resistant to macrophage-mediated degradation.

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References 54 · Cited by 356

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Authors
Valentin, J. E., Stewart-Akers, A. M., Gilbert, T. W., & Badylak, S. F.
Affiliations
  • University of Pittsburgh (US)
  • Department of Surgery, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania.
Corresponding author
Badylak, Stephen F.
ORCID
Venue
Tissue Engineering Part A
Publisher
SAGE Publications
Published
2009-07
Volume
15
Issue
7
Pages
1687-1694
ISSN
  • 1937-3341 (ISSN-L)
  • 1937-335X
DOI
10.1089/ten.tea.2008.0419
PMC
PMC2792102
OpenAlex
W2053475436
Cited by
356
References
54
Citations by year
2026: 7 · 2025: 15 · 2024: 14 · 2023: 13 · 2022: 18 · 2021: 29 · 2020: 15 · 2019: 19 · 2018: 18 · 2017: 31 · 2016: 21 · 2015: 29 · 2014: 34 · 2013: 30 · 2012: 20
FWCI
20.95
Type
journal article
Language
en
Open access
Licence
www.liebertpub.com/nv/resources-tools/text-and-data-mining-policy/121/ (tdm)
Locations
  • Tissue Engineering Part A (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of Biomedical Imaging and Bioengineering (EB007678-01)
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
  • Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
Keywords
macrophages, extracellular matrix scaffolds, scaffold degradation, tissue remodeling, small intestinal submucosa, carbodiimide crosslinking, clodronate liposomes, macrophage depletion, peripheral blood monocytes, host tissue response
MeSH terms
CD68 Molecule, Animals, Carbodiimides, Cross-Linking Reagents, Extracellular Matrix, Fluorescent Antibody Technique, Immunohistochemistry, Intestinal Mucosa, Macrophages, Male, Postoperative Care, Transplantation, Autologous, Antigens, Differentiation, Myelomonocytic, Antigens, CD, Prosthesis Implantation, Sus scrofa, Rats, Tissue Scaffolds

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