Plant-derived human collagen scaffolds for skin tissue engineering
Open accessAbstract
Tissue engineering scaffolds are commonly formed using proteins extracted from animal tissues, such as bovine hide. Risks associated with the use of these materials include hypersensitivity and pathogenic contamination. Human-derived proteins lower the risk of hypersensitivity, but possess the risk of disease transmission. Methods engineering recombinant human proteins using plant material provide an alternate source of these materials without the risk of disease transmission or concerns regarding variability. To investigate the utility of plant-derived human collagen (PDHC) in the development of engineered skin (ES), PDHC and bovine hide collagen were formed into tissue engineering scaffolds using electrospinning or freeze-drying. Both raw materials were easily formed into two common scaffold types, electrospun nonwoven scaffolds and lyophilized sponges, with similar architectures. The processing time, however, was significantly lower with PDHC. PDHC scaffolds supported primary human cell attachment and proliferation at an equivalent or higher level than the bovine material. Interleukin-1 beta production was significantly lower when activated THP-1 macrophages where exposed to PDHC electrospun scaffolds compared to bovine collagen. Both materials promoted proper maturation and differentiation of ES. These data suggest that PDHC may provide a novel source of raw material for tissue engineering with low risk of allergic response or disease transmission.
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Collagen extracted from mammalian sources may lead to the transmission of disease to humans.
“The collagen extracted from mammalian sources may lead to the transmission of the disease to humans [118].”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [118]
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References 49 · Cited by 87
Research works printed but not linked: 3
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- Authors
- Willard, J. J., Drexler, J. W., Das, A., Roy, S., Shilo, S., Shoseyov, O., & Powell, H. M.
- Affiliations
- The Ohio State University (US)
- Collplant Ltd.
- Hebrew University of Jerusalem (IL)
- Corresponding author
- Powell, Heather M.
- Venue
- Tissue Engineering Part A
- Publisher
- SAGE Publications
- Published
- 2013-02-18
- Volume
- 19
- Issue
- 13-14
- Pages
- 1507-1518
- ISSN
- 1937-3341 (ISSN-L)
- 1937-335X
- PMC
- PMC3665310
- OpenAlex
- W2030613766
- Cited by
- 87
- References
- 49
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 8 · 2023: 11 · 2022: 9 · 2021: 9 · 2020: 7 · 2019: 10 · 2018: 4 · 2017: 3 · 2016: 6 · 2015: 3 · 2014: 5 · 2013: 3
- FWCI
- 2.16
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Tissue Engineering Part A (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation (0914790)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK076566)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- tissue engineering scaffolds, cell adhesion and proliferation, IL-1β, THP-1 macrophages, disease transmission risk, allergic reactions
- MeSH terms
- Biocompatible Materials, Cells, Cultured, Collagen, Humans, Materials Testing, Plants, Skin, Tissue Engineering, Tissue Scaffolds
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