1-Step Versus 2-Step Immobilization of Alkaline Phosphatase and Bone Morphogenetic Protein-2 onto Implant Surfaces Using Polydopamine
Open accessAbstract
Immobilization of biomolecules onto implant surfaces is highly relevant in many areas of biomaterial research. Recently, a 2-step immobilization procedure was developed for the facile conjugation of biomolecules onto various surfaces using self-polymerization of dopamine into polydopamine. In the current study, a 1-step polydopamine-based approach was applied for alkaline phosphatase (ALP) and bone morphogenetic protein-2 (BMP-2) immobilization, and compared to the conventional 2-step polydopamine-based immobilization and plain adsorption. To this end, ALP and BMP-2 were immobilized onto titanium and polytetrafluoroethylene (PTFE) substrates. The absolute quantity and biological activity of immobilized ALP were assessed quantitatively to compare the three types of immobilization. Plain adsorption of both ALP and BMP-2 was inferior to both polydopamine-based immobilization approaches. ALP was successfully immobilized onto titanium and PTFE surfaces via the 1-step approach, and the immobilized ALP retained its enzymatic activity. Using the 1-step approach, the amount of immobilized ALP was increased twofold to threefold compared to the conventional 2-step immobilization process. In contrast, more BMP-2 was immobilized using the conventional 2-step immobilization approach. Retention of ALP and BMP-2 was measured over a period of 4 weeks and was found to be similar for the 1-step and 2-step methods and far superior to the retention of adsorbed biomolecules due to the formation of…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Compared with traditional immobilization approaches, PDA-assisted immobilization significantly improves the anchoring performance of biomolecules.
“Compared to traditional immobilization approaches, PDA-assisted immobilization exhibits a merit to significantly improve the anchoring performance of biomolecules [79].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [79]
Cites
The effect of VEGF functionalization of titanium on endothelial cells in vitro
Poh, C. K., Shi, Z., Lim, T. Y., Neoh, K. G., & Wang, W. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2009.11.042 · Cited by 236 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Polydopamine-Assisted Surface Modification for Bone Biosubstitutes
Huang, S., Liang, N., Hu, Y., Zhou, X., & Abidi, N. (2016) · BioMed Research International · doi:10.1155/2016/2389895 · Cited by 97 · see citing works
“Studies have shown that the activity of these biomolecules was not affected by the PDA coating layer itself except for the forming condition of PDA coating layer and the inherent properties of biomolecules (incubation time, concentration, molecule weight, and isoelectric point) [78].”
Background
- Authors
- Nijhuis, A. W. G., van den Beucken, J. J. J. P., Boerman, O. C., Jansen, J. A., & Leeuwenburgh, S. C. G.
- Affiliations
- Radboud University Medical Center (NL)
- Department of Biomaterials, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
- Corresponding author
- Leeuwenburgh, Sander C.G.
- Venue
- Tissue Engineering Part C: Methods
- Publisher
- SAGE Publications
- Published
- 2013-08
- Volume
- 19
- Issue
- 8
- Pages
- 610-619
- ISSN
- 1937-3384 (ISSN-L)
- 1937-3392
- PMC
- PMC3689932
- OpenAlex
- W2124349212
- Cited by
- 37
- References
- 1
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 3 · 2018: 4 · 2017: 3 · 2016: 6 · 2015: 4 · 2014: 2 · 2013: 1
- FWCI
- 0.99
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Tissue Engineering Part C Methods (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Radboud Repository (Radboud University) (submittedVersion)
- Funders
- Koninklijke Nederlandse Akademie van Wetenschappen
- Topics
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Engineering › Mechanical Engineering › Surface Treatment and Coatings
- Keywords
- polydopamine, alkaline phosphatase, BMP-2, enzyme immobilization, biomolecule immobilization, titanium implants, polytetrafluoroethylene, adsorption, implant coatings
- MeSH terms
- Alkaline Phosphatase, Animals, Bone Marrow Cells, Cattle, Cell Adhesion, Cells, Cultured, Enzymes, Immobilized, Humans, Indoles, Polymers, Polytetrafluoroethylene, Titanium, Prostheses and Implants, Coated Materials, Biocompatible, Rats, Bone Morphogenetic Protein 2
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
