Wnt family proteins are secreted and associated with the cell surface.
Open accessAbstract
Members of the Wnt gene family are proposed to function in both normal development and differentiation as well as in mammary tumorigenesis. To understand the function of Wnt proteins in these two processes, we present here a biochemical characterization of seven Wnt family members. For these studies, AtT-20 cells, a neuroendocrine cell line previously shown to efficiently process and secrete Wnt-1, was transfected with expression vectors encoding Wnt family members. All of the newly characterized Wnt proteins are glycosylated, secreted proteins that are tightly associated with the cell surface or extracellular matrix. We have also identified native Wnt proteins in retinoic acid-treated P19 embryonal carcinoma cells, and they exhibit the same biochemical characteristics as the recombinant proteins. These data suggest that Wnt family members function in cell to cell signaling in a fashion similar to Wnt-1.
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The Wnt proteins are glycosylated in the endoplasmic reticulum and also are palmitolated.
“Studies have revealed that the Wnt proteins are glycosylated in the endoplasmic reticulum and also are palmitolated.9,10”
From Wnt signal transduction pathways (2008) · cites this paper as [9]
Cites
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
Felgner, P. L., Gadek, T. R., Holm, M., Roman, R., Chan, H. W., Wenz, M., Northrop, J. P., RINGOLD, G. M., & Danielsen, M. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.21.7413 · Cited by 4,954 · see citing works
Southern, P. J., & Berg, P. R. (1982) · PubMed · Cited by 2,740 · see citing works
Nusse, R., & VARMUS, H. E. (1982) · Cell · doi:10.1016/0092-8674(82)90409-3 · Cited by 1,864 · see citing works
The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain
McMahon, A. P., & Bradley, A. (1990) · Cell · doi:10.1016/0092-8674(90)90385-r · Cited by 1,540 · see citing works
Self-citationRijsewijk, F. A. M., Schuermann, M., Wagenaar, E., Parren, P. W. H. I., Weigel, D., & Nusse, R. (1987) · Cell · doi:10.1016/0092-8674(87)90038-9 · Cited by 931 · see citing works
Thomas, K. R., & Capecchi, M. R. (1990) · Nature · doi:10.1038/346847a0 · Cited by 896 · see citing works
Nusse, R., & VARMUS, H. E. (1992) · Cell · doi:10.1016/0092-8674(92)90630-u · Cited by 814 · see citing works
Tsukamoto, A. S., Grosschedl, R., Guzman, R. C., Parslow, T. G., & Varmus, H. E. (1988) · Cell · doi:10.1016/0092-8674(88)90220-6 · Cited by 747 · see citing works
Smith, W. C., & Harland, R. M. (1991) · Cell · doi:10.1016/0092-8674(91)90070-f · Cited by 649 · see citing works
Wilkinson, D. G., Bailes, J. A., & McMahon, A. P. (1987) · Cell · doi:10.1016/0092-8674(87)90664-7 · Cited by 580 · see citing works
Self-citationMcMahon, A. P., & Moon, R. T. (1989) · Cell · doi:10.1016/0092-8674(89)90506-0 · Cited by 530 · see citing works
Self-citationInjected Wnt RNA induces a complete body axis in Xenopus embryos
Sokol, S. Y., Christian, J. L., Moon, R. T., & Melton, D. A. (1991) · Cell · doi:10.1016/0092-8674(91)90069-b · Cited by 501 · see citing works
Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development.Preprint
Gavin, B. J., McMahon, J. A., & McMahon, A. P. (1990) · Genes & Development · doi:10.1101/gad.4.12b.2319 · Cited by 455 · see citing works
Self-citationChristian, J. L., McMahon, J. A., McMahon, A. P., & Moon, R. T. (1991) · Development · doi:10.1242/dev.111.4.1045 · Cited by 374 · see citing works
Self-citationThe proto‐oncogene int‐1 encodes a secreted protein associated with the extracellular matrix.
Bradley, R. S., & Brown, A. M. C. (1990) · The EMBO Journal · doi:10.1002/j.1460-2075.1990.tb08276.x · Cited by 277 · see citing works
Shackleford, G. M., & VARMUS, H. E. (1987) · Cell · doi:10.1016/0092-8674(87)90665-9 · Cited by 246 · see citing works
Swaying is a mutant allele of the proto-oncogene Wnt-1
Thomas, K. R., Musci, T. S., Neumann, P. E., & Capecchi, M. R. (1991) · Cell · doi:10.1016/0092-8674(91)90369-a · Cited by 243 · see citing works
Secretion and movement of wingless protein in the epidermis of the Drosophila embryo
González, F., Swales, L. S., Bejsovec, A., Skaer, H., & Arias, A. M. (1991) · Mechanisms of Development · doi:10.1016/0925-4773(91)90040-d · Cited by 238 · see citing works
Secreted int-1 protein is associated with the cell surface.
Papkoff, J., & Schryver, B. B. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.6.2723 · Cited by 212 · see citing works
Self-citationOverlapping Expression of Xwnt-3A and Xwnt-1 in Neural Tissue of Xenopus laevis Embryos
Wolda, S. L., Moody, C. J., & Moon, R. T. (1993) · Developmental Biology · doi:10.1006/dbio.1993.1005 · Cited by 201 · see citing works
Brown, A. M. C., Wildin, R. S., Prendergast, T. J., et al. (1986) · Cell · doi:10.1016/0092-8674(86)90699-9 · Cited by 190 · see citing works
Roelink, H., Wagenaar, E., da Silva, S. L., & Nusse, R. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.12.4519 · Cited by 187 · see citing works
Gavin, B. J., & McMahon, A. P. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.5.2418 · Cited by 152 · see citing works
Self-citationThe Mouse Wnt-1 Gene Can Act Via a Paracrine Mechanism in Transformation of Mammary Epithelial Cells
Jue, S. F., Bradley, R. S., Rudnicki, J. A., VARMUS, H. E., & Brown, A. M. C. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.1.321-328.1992 · Cited by 147 · see citing works
The int -1 Proto-Oncogene Products Are Glycoproteins That Appear To Enter the Secretory Pathway
Papkoff, J., Brown, A. M. C., & VARMUS, H. E. (1987) · Molecular and Cellular Biology · doi:10.1128/mcb.7.11.3978-3984.1987 · Cited by 142 · see citing works
Self-citationEffect of Wnt-1 and Related Proteins on Gap Junctional Communication in Xenopus Embryos
Olson, D. J., Christian, J. L., & Moon, R. T. (1991) · Science · doi:10.1126/science.252.5009.1173 · Cited by 128 · see citing works
Diversification of the Wnt gene family on the ancestral lineage of vertebrates.
Sidow, A. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.11.5098 · Cited by 124 · see citing works
Rijsewijk, F. A. M., van Deemter, L., Wagenaar, E., et al. (1987) · The EMBO Journal · doi:10.1002/j.1460-2075.1987.tb04729.x · Cited by 119 · see citing works
Mason, J. O., Kitajewski, J., & VARMUS, H. E. (1992) · Molecular Biology of the Cell · doi:10.1091/mbc.3.5.521 · Cited by 111 · see citing works
Wainwright, B. J., Scambler, P. J., Stanier, P., et al. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb03003.x · Cited by 96 · see citing works
The int-1 proto-oncogene products are glycoproteins that appear to enter the secretory pathway.
Papkoff, J., Brown, A. M., & VARMUS, H. E. (1987) · Molecular and Cellular Biology · doi:10.1128/mcb.7.11.3978 · Cited by 92 · see citing works
Self-citationIdentification of protein products encoded by the proto-oncogene int-1.
Brown, A. M. C., Papkoff, J., Fung, Y. K., et al. (1987) · Molecular and Cellular Biology · doi:10.1128/mcb.7.11.3971 · Cited by 82 · see citing works
Self-citationIsolation and expression of two novel Wnt/wingless gene homologues in Drosophila
Russell, J., Gennissen, A. M. C., & Nusse, R. (1992) · Development · doi:10.1242/dev.115.2.475 · Cited by 61 · see citing works
Inducible overexpression and secretion of int-1 protein.
Papkoff, J. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.8.3377 · Cited by 60 · see citing works
Self-citationThe biochemical properties and transforming potential of human Wnt-2 are similar to Wnt-1.
Blasband, A. J., Schryver, B. B., & Papkoff, J. (1992) · PubMed · Cited by 46 · see citing works
Self-citationEisenberg, L. M., Ingham, P. W., & Brown, A. M. C. (1992) · Developmental Biology · doi:10.1016/0012-1606(92)90049-m · Cited by 44 · see citing works
The Wnt family of developmental regulators
McMahon, A. P. (1992) · Trends in Genetics · doi:10.1016/0168-9525(92)90122-k · Cited by 37 · see citing works
Self-citationSt‐Arnaud, R., Craig, J., Mw, M., et al. (1989) · PubMed · Cited by 33 · see citing works
Identification of Protein Products Encoded by the Proto-Oncogene int-1
Brown, A. M., Papkoff, J., Fung, Y. K., et al. (1987) · Molecular and Cellular Biology · doi:10.1128/mcb.7.11.3971-3977.1987 · Cited by 28 · see citing works
Self-citation(1990) · Trends in Genetics · doi:10.1016/0168-9525(90)90263-6 · Cited by 21 · see citing works
Papkoff, J. (1994) · PubMed · Cited by 18 · see citing works
Self-citationInjected Wnt RNA induces a complete body axis in Xenopus embryos
(1992) · Trends in Genetics · doi:10.1016/0168-9525(92)90344-4 · Cited by 16 · see citing works
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Obermeier, B., Daneman, R., & Ransohoff, R. M. (2013) · Nature Medicine · doi:10.1038/nm.3407 · Cited by 2,452 · see citing works
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Komiya, Y., & Habas, R. (2008) · Organogenesis · doi:10.4161/org.4.2.5851 · Cited by 1,528 · see citing works
Kireeva, M. L., Mo, F.-E., Yang, G. P., & Lau, L. F. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.4.1326 · Cited by 341 · see citing works
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- Authors
- Smolich, B. D., McMahon, J. A., McMahon, A. P., & Papkoff, J.
- Affiliations
- SUGEN, Redwood City, California 94063.
- ORCID
- McMahon, A P
- Venue
- Molecular Biology of the Cell
- Published
- 1993-12
- Volume
- 4
- Issue
- 12
- Pages
- 1267-1275
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC275763
- OpenAlex
- W1973516848
- Cited by
- 172
- References
- 42
- Citations by year
- 2025: 6 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 6 · 2018: 7 · 2017: 16 · 2016: 7 · 2015: 4 · 2014: 7 · 2013: 6 · 2012: 10
- FWCI
- 1.29
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular Biology of the Cell (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Hedgehog Signaling Pathway Studies
- Keywords
- Wnt proteins, Wnt1, protein secretion, glycosylation, extracellular matrix, mammary carcinogenesis, cell-to-cell signaling, retinoic acid, P19 embryonal carcinoma cells, AtT-20 cells, developmental signaling
- MeSH terms
- Amino Acid Sequence, Animals, Cell Membrane, Extracellular Matrix, Multigene Family, Glycoproteins, Glycosylation, Molecular Sequence Data, Neoplasm Proteins, Pituitary Neoplasms, Protein Processing, Post-Translational, Proteins, Proto-Oncogene Proteins, Recombinant Fusion Proteins, Transfection, Tumor Cells, Cultured, Signal Transduction, Carcinoma, Embryonal, Zebrafish Proteins, Wnt Proteins, Wnt2 Protein, Wnt1 Protein, Mice, Wnt3 Protein
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