Cited by 172Wnt/β-catenin signaling in development and cancerDevelopmental Biology and Gene RegulationHedgehog Signaling Pathway Studies

Wnt family proteins are secreted and associated with the cell surface.

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Smolich, B D, McMahon, J A, McMahon, A P, Papkoff, J·1993·Molecular Biology of the Cell

Abstract

Members of the Wnt gene family are proposed to function in both normal development and differentiation as well as in mammary tumorigenesis. To understand the function of Wnt proteins in these two processes, we present here a biochemical characterization of seven Wnt family members. For these studies, AtT-20 cells, a neuroendocrine cell line previously shown to efficiently process and secrete Wnt-1, was transfected with expression vectors encoding Wnt family members. All of the newly characterized Wnt proteins are glycosylated, secreted proteins that are tightly associated with the cell surface or extracellular matrix. We have also identified native Wnt proteins in retinoic acid-treated P19 embryonal carcinoma cells, and they exhibit the same biochemical characteristics as the recombinant proteins. These data suggest that Wnt family members function in cell to cell signaling in a fashion similar to Wnt-1.

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Crossref · 10.1091/mbc.4.12.1267

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  • The Wnt proteins are glycosylated in the endoplasmic reticulum and also are palmitolated.

    “Studies have revealed that the Wnt proteins are glycosylated in the endoplasmic reticulum and also are palmitolated.9,10”

    From Wnt signal transduction pathways (2008) · cites this paper as [9]

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Authors
Smolich, B. D., McMahon, J. A., McMahon, A. P., & Papkoff, J.
Affiliations
SUGEN, Redwood City, California 94063.
Venue
Molecular Biology of the Cell
Published
1993-12
Volume
4
Issue
12
Pages
1267-1275
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
OpenAlex
W1973516848
Cited by
172
References
42
Citations by year
2025: 6 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 6 · 2018: 7 · 2017: 16 · 2016: 7 · 2015: 4 · 2014: 7 · 2013: 6 · 2012: 10
FWCI
1.29
Type
journal article
Language
en
Open access
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Hedgehog Signaling Pathway Studies
Keywords
Wnt proteins, Wnt1, protein secretion, glycosylation, extracellular matrix, mammary carcinogenesis, cell-to-cell signaling, retinoic acid, P19 embryonal carcinoma cells, AtT-20 cells, developmental signaling
MeSH terms
Amino Acid Sequence, Animals, Cell Membrane, Extracellular Matrix, Multigene Family, Glycoproteins, Glycosylation, Molecular Sequence Data, Neoplasm Proteins, Pituitary Neoplasms, Protein Processing, Post-Translational, Proteins, Proto-Oncogene Proteins, Recombinant Fusion Proteins, Transfection, Tumor Cells, Cultured, Signal Transduction, Carcinoma, Embryonal, Zebrafish Proteins, Wnt Proteins, Wnt2 Protein, Wnt1 Protein, Mice, Wnt3 Protein

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