Cited by 151Cellular transport and secretionCalcium signaling and nucleotide metabolismAutophagy in Disease and Therapy

Interorganellar Regulation of Lysosome Positioning by the Golgi Apparatus through Rab34 Interaction with Rab-interacting Lysosomal Protein

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Wang, Tuanlao, Hong, Wanjin·2002·Molecular Biology of the Cell

Abstract

We present evidence to suggest the existence of a regulatory pathway for the Golgi apparatus to modulate the spatial positioning of otherwise distantly located lysosomes. Rab34, a new member of the Rab GTPase family, is associated primarily with the Golgi apparatus. Expression of wild-type or GTP-restricted but not GDP-restricted versions of Rab34 causes spatial redistribution of lysosomes from the periphery to the peri-Golgi region. The regulation of lysosomal positioning by Rab34 depends on its association with the membrane mediated by prenylation and its direct interaction with Rab-interacting lysosomal protein (RILP). This biological activity, mediated by Rab34-RILP interaction, is dependent on Lys82 in the switch I region. Our results have uncovered a novel mechanism for the Golgi apparatus to regulate the spatial distribution of another organelle.

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Crossref · 10.1091/mbc.e02-05-0280

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  • There was a very high location similarity between lysosomes and Golgi.

    “We found a very high location similarity between lysosomes and Golgi, consistent with their enrichment in location in the apical cytoplasm and the known role of the Golgi in regulating lysosome localization20,21.”

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Authors
Wang, T., & Hong, W.
Affiliations
Institute of Molecular and Cell Biology (SG)
Venue
Molecular Biology of the Cell
Published
2002-12
Volume
13
Issue
12
Pages
4317-4332
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
OpenAlex
W2137315893
Cited by
147
References
48
Citations by year
2026: 4 · 2025: 6 · 2024: 11 · 2023: 6 · 2022: 8 · 2021: 9 · 2020: 6 · 2019: 4 · 2018: 7 · 2017: 13 · 2016: 6 · 2015: 5 · 2014: 8 · 2013: 3 · 2012: 6
FWCI
2.19
Type
journal article
Language
en
Open access
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National University of Singapore
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
Keywords
lysosome positioning, Golgi apparatus, Rab34, Rab GTPases, RILP, prenylation, switch I region
MeSH terms
Amino Acid Sequence, Animals, Axons, Base Sequence, Carrier Proteins, Cells, Cultured, Cloning, Molecular, Cytoskeleton, Glutathione Transferase, Golgi Apparatus, HeLa Cells, Humans, Lysine, Lysosomes, Microscopy, Fluorescence, Models, Genetic, Molecular Sequence Data, Plant Proteins, Protein Binding, Transfection, Mutagenesis, Site-Directed, Sequence Homology, Amino Acid, DNA, Complementary, Fluorescent Antibody Technique, Indirect, rab GTP-Binding Proteins, Two-Hybrid System Techniques, Adaptor Proteins, Signal Transducing, Mice, Rats

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