The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
The Role of Rab27a in the Regulation of Melanosome Distribution within Retinal Pigment Epithelial Cells
Open accessAbstract
Melanosomes within the retinal pigment epithelium (RPE) of mammals have long been thought to exhibit no movement in response to light, unlike fish and amphibian RPE. Here we show that the distribution of melanosomes within the mouse RPE undergoes modest but significant changes with the light cycle. Two hours after light onset, there is a threefold increase in the number of melanosomes in the apical processes that surround adjacent photoreceptors. In skin melanocytes, melanosomes are motile and evenly distributed throughout the cell periphery. This distribution is due to the interaction with the cortical actin cytoskeleton mediated by a tripartite complex of Rab27a, melanophilin, and myosin Va. In ashen (Rab27a null) mice RPE, melanosomes are unable to move beyond the adherens junction axis and do not enter apical processes, suggesting that Rab27a regulates melanosome distribution in the RPE. Unlike skin melanocytes, the effects of Rab27a are mediated through myosin VIIa in the RPE, as evidenced by the similar melanosome distribution phenotype observed in shaker-1 mice, defective in myosin VIIa. Rab27a and myosin VIIa are likely to be required for association with and movement through the apical actin cytoskeleton, which is a prerequisite for entry into the apical processes.
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“myosin-7a associates with RPE melanosomes by binding to myosin-7a- and Rab-interacting protein (MYRIP) (El-Amraoui et al., 2002), which in turn binds to RAB27A on the melanosome surface (Futter et al., 2004; Gibbs et al., 2004; Kuroda and Fukuda, 2005; Klomp et al., 2007)”
From The cell biology of the retinal pigment epithelium (2020) · cites this paper as Futter et al., 2004
In the citing paper
“Although more muted, melanosomes have been observed to move into the RPE apical processes in response to light onset (Futter et al., 2004).”
From The cell biology of the retinal pigment epithelium (2020) · cites this paper as Futter et al., 2004
In the citing paper
“Interestingly, mutant CHM results in underprenylated RAB27A (Seabra et al., 1995), which is the RAB that links myosin-7a to melanosomes (Futter et al., 2004; Gibbs et al., 2004).”
From Microtubule motors transport phagosomes in the RPE, and lack of KLC1 leads to AMD-like pathogenesis (2015) · cites this paper as Futter et al., 2004
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Citations
Cites
Defective myosin VIIA gene responsible for Usher syndrome type IB
Well, D., Blanchard, S., Kaplan, J., Guilford, P., Gibson, F., Walsh, J., Mburu, P., Varela, A., Levilliers, J., Weston, M. D., et al. (1995) · Nature · doi:10.1038/374060a0 · Cited by 1,068
Slot, J. W., Geuze, H. J., Gigengack, S., Lienhard, G. E., & James, D. E. (1991) · The Journal of Cell Biology · doi:10.1083/jcb.113.1.123 · Cited by 862
A type VII myosin encoded by the mouse deafness gene shaker-1
Gibson, F., Walsh, J. C., Mburu, P., Varela, A., Brown, K. A., Antonio, M., Beisel, K. W., Steel, K. P., & Brown, S. D. M. (1995) · Nature · doi:10.1038/374062a0 · Cited by 682
Novel myosin heavy chain encoded by murine dilute coat colour locus
Mercer, J. A., Seperack, P. K., Strobel, M. C., Copeland, N. G., & Jenkins, N. A. (1991) · Nature · doi:10.1038/349709a0 · Cited by 536
Rab GTPases, intracellular traffic and disease
Seabra, M. C., Mules, E. H., & Hume, A. N. (2002) · Trends in Molecular Medicine · doi:10.1016/s1471-4914(01)02227-4 · Cited by 473
Self-citationIdentification of an organelle receptor for myosin-Va
Wu, X., Rao, K., Zhang, H., Wang, F., Sellers, J. R., Matesic, L. E., Copeland, N. G., Jenkins, N. A., & Hammer, J. A. (2002) · Nature Cell Biology · doi:10.1038/ncb760 · Cited by 455
Microtubules containing detyrosinated tubulin are less dynamic.
Kreis, T. E. (1987) · The EMBO Journal · doi:10.1002/j.1460-2075.1987.tb02550.x · Cited by 431
Wu, X., Bowers, B., Rao, K., Wei, Q., & Hammer, J. A. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.143.7.1899 · Cited by 419
A mutation in Rab27a causes the vesicle transport defects observed in ashen mice
Wilson, S. M., Yip, R., Swing, D. A., O’Sullivan, T. N., Zhang, Y., Novak, E. K., Swank, R. T., Russell, L. B., Copeland, N. G., & Jenkins, N. A. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.140212797 · Cited by 406
Slac2-a/Melanophilin, the Missing Link between Rab27 and Myosin Va
Fukuda, M., Kuroda, T. S., & Mikoshiba, K. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.c200005200 · Cited by 364
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Research works printed but not linked: 2
- Burnside, B., and Bost-Usinger, L. (1998). The retinal pigment epithelium cytoskeleton. In: The Retinal Pigment Epithelium: Function and Disease , eds. J.F. Marmor and T.J. Wolfensberger, Oxford, UK: Oxford University Press, 41–67.
- Burnside, B., and Laties, A.M. (1979). Pigment movement and cellular contractility in the retinal pigment epithelium. In: The Retinal Pigment Epithelium , eds. K.M. Zinn and M.F. Marmor, Cambridge, MA: Harvard University Press, 175–191.
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Items 101–110 of 110
Shelby, S. J. (2012) · Deep Blue (University of Michigan)
Statins and Protein Prenylation in the Retina
Ling, J. Y. M. (2017) · ERA: Education and Research Archive (University of Alberta) · doi:10.7939/r39s1m03v
Retinal morphology and retinomotor response in Caspian kutum (Rutilus frisii subsp. kutum)
Abbasi, M., & Shabanipour, N. (2019) · Nova Biologica Reperta · doi:10.29252/nbr.5.4.356
A method for quantifying pigment position in retinal pigment epithelium
Ibarra, D. E., Wiebe, D. E., Mireles, T., Pattillo, A., Roberts, T., Wood, T. S., Przybylski, C., Rodriguez, C. J., & Garcia, D. (2020) · Experimental Eye Research · doi:10.1016/j.exer.2020.108038
Sabry, D. A., & El-Badry, D. A. (2020) · Zoologia (Curitiba) · doi:10.3897/zoologia.37.e51603
Fukuda, M., & Ohbayashi, N. (2020) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.735464281.793574979
Cellular Organization of the Retinal Pigment Epithelium
Burgoyne, T., & Futter, C. E. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-13820-1.00067-0
Self-citationGermer, C. J., Williams, S., Fernandes, V., Lima, R. E., Cunza, N. L., Tan, L. X., Panda, S. R., Iorio, E., Elahi, F. M., & Lakkaraju, A. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.02.20.706984
Rôle du facteur H du complément et du récepteur CD47 dans l’inflammation sous-rétinienne
Augustin, S. (2021) · theses.fr (ABES) · doi:10.70675/be8ffdbez2125z4378za1bbzb8ba72c35267
Metadata
- Authors
- Futter, C. E., Ramalho, J. S., Jaissle, G. B., Seeliger, M. W., & Seabra, M. C.
- Affiliations
- University College London (GB)
- Centre of Ophthalmology, Biomedical Institute for Research in Light and Image, University of Coimbra, 3000-354 Coimbra, Portugal
- University of Tübingen (DE)
- Imperial College London (GB)
- Venue
- Molecular Biology of the Cell
- Published
- 2004-05
- Volume
- 15
- Issue
- 5
- Pages
- 2264-2275
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC404021
- OpenAlex
- W2121707887
- Cited by
- 110
- References
- 39
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 5 · 2023: 8 · 2022: 5 · 2021: 3 · 2020: 9 · 2019: 8 · 2018: 4 · 2017: 1 · 2016: 4 · 2015: 7 · 2014: 3 · 2013: 3 · 2012: 2
- FWCI
- 3.57
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Molecular Biology of the Cell (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- Foundation Fighting Blindness
- Wellcome Trust
- Deutsche Forschungsgemeinschaft
- Moorfields Eye Hospital NHS Foundation Trust
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Health Sciences › Medicine › Ophthalmology › Retinal Development and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- Rab27a, melanosomes, retinal pigment epithelium, MYO7A, myosin Va, melanophilin, cortical actin cytoskeleton, light regulation, adherens junctions, apical processes, melanosome transport
- MeSH terms
- rab27 GTP-Binding Proteins, Animals, Cytoskeletal Proteins, Electrophysiology, Mice, Mutant Strains, Microscopy, Pigment Epithelium of Eye, Retina, Melanosomes, rab GTP-Binding Proteins, Mice
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