The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
ATM-dependent DNA Damage-independent Mitotic Phosphorylation of H2AX in Normally Growing Mammalian Cells
Open accessAbstract
H2AX is a core histone H2A variant that contains an absolutely conserved serine/glutamine (SQ) motif within an extended carboxy-terminal tail. H2AX phosphorylation at the SQ motif (γ-H2AX) has been shown to increase dramatically upon exogenously introduced DNA double-strand breaks (DSBs). In this study, we use quantitative in situ approaches to investigate the spatial patterning and cell cycle dynamics of γ-H2AX in a panel of normally growing (unirradiated) mammalian cell lines and cultures. We provide the first evidence for the existence of two distinct yet highly discernible γ-H2AX focal populations: a small population of large amorphous foci that colocalize with numerous DNA DSB repair proteins and previously undescribed but much more abundant small foci. These small foci do not recruit proteins involved in DNA DSB repair. Cell cycle analyses reveal unexpected dynamics for γ-H2AX in unirradiated mammalian cells that include an ATM-dependent phosphorylation that is maximal during M phase. Based upon similarities drawn from other histone posttranslational modifications and previous observations in haploinsufficient (H2AX-/+) and null mice (H2AX-/-), γ-H2AX may contribute to the fidelity of the mitotic process, even in the absence of DNA damage, thereby ensuring the faithful transmission of genetic information from one generation to the next.
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Cites
DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139
Rogakou, E. P., Pilch, D. R., Orr, A. H., Ivanova, V. S., & Bonner, W. M. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.10.5858 · Cited by 5,438
DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation
Bakkenist, C. J., & Kastan, M. B. (2003) · Nature · doi:10.1038/nature01368 · Cited by 3,466
The DNA damage response: putting checkpoints in perspective
Zhou, B.-B. S., & Elledge, S. J. (2000) · Nature · doi:10.1038/35044005 · Cited by 3,213
ATM Phosphorylates Histone H2AX in Response to DNA Double-strand Breaks
Burma, S., Chen, B. P., Murphy, M., Kurimasa, A., & Chen, D. J. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.c100466200 · Cited by 1,954
Genomic Instability in Mice Lacking Histone H2AX
Celeste, A., Petersen, S., Romanienko, P. J., Fernandez-Capetillo, O., Chen, H. T., Sedelnikova, O. A., Reina-San-Martin, B., Coppola, V., Meffre, E., Difilippantonio, M. J., et al. (2002) · Science · doi:10.1126/science.1069398 · Cited by 1,385
Dynamics of DNA Double-Strand Breaks Revealed by Clustering of Damaged Chromosome Domains
Aten, J. A., Stap, J., Krawczyk, P. M., van Oven, C. H., Hoebe, R. A., Essers, J., & Kanaar, R. (2004) · Science · doi:10.1126/science.1088845 · Cited by 491
Limoli, C. L., Giedzinski, E., Bonner, W. M., & Cleaver, J. E. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.231611798 · Cited by 210
Cited by
Bonner, W. M., Redon, C. E., Dickey, J. S., Nakamura, A. J., Sedelnikova, O. A., Solier, S., & Pommier, Y. (2008) · Nature reviews. Cancer · doi:10.1038/nrc2523 · Cited by 1,623
Lysosome-mediated processing of chromatin in senescence
Ivanov, A., Pawlikowski, J., Manoharan, I., van Tuyn, J., Nelson, D. M., Rai, T. S., Shah, P. P., Hewitt, G., Korolchuk, V. I., Passos, J. F., et al. (2013) · Journal of Cell Biology · doi:10.1083/jcb.201212110 · Cited by 539
Lambrot, R., Xu, C., Saint-Phar, S., Chountalos, G., Cohen, T., Paquet, M., Suderman, M., Hallett, M., & Kimmins, S. (2013) · Nature Communications · doi:10.1038/ncomms3889 · Cited by 473
van der Burg, M., IJspeert, H., Verkaik, N. S., Turul, T., Wiegant, W. W., Morotomi-Yano, K., Mari, P.-O., Tezcan, I., Chen, D. J., Zdzienicka, M. Z., et al. (2008) · Journal of Clinical Investigation · doi:10.1172/jci37141 · Cited by 289
BMI1-mediated histone ubiquitylation promotes DNA double-strand break repair
Ismail, I. H., Andrin, C., McDonald, D., & Hendzel, M. J. (2010) · Journal of Cell Biology · doi:10.1083/jcb.201003034 · Cited by 255
Self-citation
Metadata
- Authors
- McManus, K. J., & Hendzel, M. J.
- Venue
- Molecular Biology of the Cell
- Published
- 2005-10
- Volume
- 16
- Issue
- 10
- Pages
- 5013-5025
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2097477376
- Cited by
- 243
- References
- 7
- FWCI
- 6.11
- Type
- journal article
- Language
- en
- Open access
- green
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- McManus, K. J., & Hendzel, M. J. (2005). ATM-dependent DNA Damage-independent Mitotic Phosphorylation of H2AX in Normally Growing Mammalian Cells. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e05-01-0065
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