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Cited by 76melanin and skin pigmentationDevelopmental Biology and Gene RegulationZebrafish Biomedical Research Applications

Pax7 is required for establishment of the xanthophore lineage in zebrafish embryos

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Nord, Hanna, Dennhag, Nils, Muck, Joscha, von Hofsten, Jonas·2016·Molecular Biology of the Cell
DOI 10.1091/mbc.e15-12-0821PMC PMC4884075
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Abstract

The pigment pattern of many animal species is a result of the arrangement of different types of pigment-producing chromatophores. The zebrafish has three different types of chromatophores: black melanophores, yellow xanthophores, and shimmering iridophores arranged in a characteristic pattern of golden and blue horizontal stripes. In the zebrafish embryo, chromatophores derive from the neural crest cells. Using pax7a and pax7b zebrafish mutants, we identified a previously unknown requirement for Pax7 in xanthophore lineage formation. The absence of Pax7 results in a severe reduction of xanthophore precursor cells and a complete depletion of differentiated xanthophores in embryos as well as in adult zebrafish. In contrast, the melanophore lineage is increased in pax7a/pax7b double-mutant embryos and larvae, whereas juvenile and adult pax7a/pax7b double-mutant zebrafish display a severe decrease in melanophores and a pigment pattern disorganization indicative of a xanthophore- deficient phenotype. In summary, we propose a novel role for Pax7 in the early specification of chromatophore precursor cells.

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Crossref · 10.1091/mbc.e15-12-0821

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  • Specification and differentiation of the third pigment cell type of zebrafish, the xanthophore, depends upon the paired-box transcription factors Pax3 and Pax7a/b.

    “Specification and differentiation of the third pigment cell type of zebrafish, the xanthophore, has been shown to depend upon the paired-box transcription factors Pax3 and Pax7a/b [59, 60].”

    From The MITF paralog tfec is required in neural crest development for fate specification of the iridophore lineage from a multipotent pigment cell progenitor (2021) · cites this paper as [60]

    The Introduction reports evidence that Pax3 is required for xanthoblast specification, and the Discussion concludes that Pax7a or Pax7b is required to establish xanthophores. The Discussion explicitly leaves unresolved whether Pax7 acts through differentiation, xanthoblast survival, or a fate decision, so the claim that differentiation depends on Pax7a/b is stronger than this paper establishes. (LLM, conf=0.86)

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Authors
Nord, H., Dennhag, N., Muck, J., & von Hofsten, J.
Affiliations
  • Umeå University (SE)
  • Max Planck Institute for Biology of Ageing (DE)
  • Umeå Centre for Molecular Medicine, Umeå University, 90187 Umeå, Sweden
ORCID
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2016-06
Volume
27
Issue
11
Pages
1853-1862
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
DOI
10.1091/mbc.e15-12-0821
PMC
PMC4884075
OpenAlex
W2328767622
Cited by
76
References
1
Citations by year
2026: 15 · 2025: 4 · 2024: 11 · 2023: 8 · 2022: 6 · 2021: 12 · 2020: 7 · 2019: 7 · 2018: 2 · 2017: 3 · 2016: 1
FWCI
2.48
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • DiVA at Umeå University (Umeå University) (submittedVersion, other-oa)
Funders
  • Royal Swedish Academy of Sciences
  • Cancerfonden
  • Magnus Bergvalls Stiftelse
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
Keywords
Pax7, xanthophores, melanophores, iridophores, neural crest, pigmentation patterns, zebrafish, double mutants
MeSH terms
Animals, Cell Differentiation, Chromatophores, Embryo, Nonmammalian, Melanophores, Neural Crest, Pigmentation, Zebrafish, Gene Expression Regulation, Developmental, Body Patterning, Zebrafish Proteins, PAX2 Transcription Factor

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