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An improved smaller biotin ligase for BioID proximity labeling
Open accessAbstract
The BioID method uses a promiscuous biotin ligase to detect protein–protein associations as well as proximate proteins in living cells. Here we report improvements to the BioID method centered on BioID2, a substantially smaller promiscuous biotin ligase. BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, and exhibits enhanced labeling of proximate proteins. Thus BioID2 improves the efficiency of screening for protein–protein associations. We also demonstrate that the biotinylation range of BioID2 can be considerably modulated using flexible linkers, thus enabling application-specific adjustment of the biotin-labeling radius.
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Cites
Extremely long-lived nuclear pore proteins in the rat brain
Savas, J. N., Toyama, B. H., Xu, T., Yates, J. R., & Hetzer, M. W. (2012) · Science · doi:10.1126/science.1217421 · Cited by 335
Novel Bilobe Components in Trypanosoma brucei Identified Using Proximity-Dependent Biotinylation
Morriswood, B., Havlicek, K., Demmel, L., Yavuz, S., Sealey-Cardona, M., Vidilaseris, K., Anrather, D., Kostan, J., Djinović-Carugo, K., Roux, K. J., et al. (2013) · Eukaryotic Cell · doi:10.1128/ec.00326-12 · Cited by 138
Self-citationBioID identifies novel c-MYC interacting partners in cultured cells and xenograft tumors
Dingar, D., Kalkat, M., Chan, P.-K., Srikumar, T., Bailey, S. D., Tu, W. B., Coyaud, E., Ponzielli, R., Kolyar, M., Jurisica, I., et al. (2015) · Journal of Proteomics · doi:10.1016/j.jprot.2014.09.029 · Cited by 131
PPP2R5C couples hepatic glucose and lipid homeostasis
Cheng, Y.-S., Seibert, O., Klöting, N., Dietrich, A., Straßburger, K., Fernández-Veledo, S., Vendrell, J. J., Zorzano, A., Blüher, M., Herzig, S., et al. (2015) · PLoS Genetics · doi:10.1371/journal.pgen.1005561 · Cited by 43
Cited by
Efficient proximity labeling in living cells and organisms with TurboID
Branon, T. C., Bosch, J. A., Sanchez, A. D., Udeshi, N. D., Svinkina, T., Carr, S. A., Feldman, J. L., Perrimon, N., & Ting, A. Y. (2018) · Nature Biotechnology · doi:10.1038/nbt.4201 · Cited by 2,174
Deciphering molecular interactions by proximity labeling
Qin, W., Cho, K. F., Cavanagh, P. E., & Ting, A. Y. (2021) · Nature Methods · doi:10.1038/s41592-020-01010-5 · Cited by 671
Proximity labeling in mammalian cells with TurboID and split-TurboID
Cho, K. F., Branon, T. C., Udeshi, N. D., Myers, S. A., Carr, S. A., & Ting, A. Y. (2020) · Nature Protocols · doi:10.1038/s41596-020-0399-0 · Cited by 534
Phosphorothioate modified oligonucleotide–protein interactions
Crooke, S. T., Vickers, T. A., & Liang, X.-H. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa299 · Cited by 372
RNA–protein interaction detection in living cells
Ramanathan, M., Majzoub, K., Rao, D. S., Neela, P. H., Zarnegar, B. J., Mondal, S., Roth, J. G., Gai, H., Kovalski, J. R., Siprashvili, Z., et al. (2018) · Nature Methods · doi:10.1038/nmeth.4601 · Cited by 308
Metadata
- Authors
- Kim, D. I., Jensen, S. C., Noble, K. A., KC, B., Roux, K. H., Motamedchaboki, K., & Roux, K. J.
- Venue
- Molecular Biology of the Cell
- Published
- 2016-04-15
- Volume
- 27
- Issue
- 8
- Pages
- 1188-1196
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2304846645
- Cited by
- 848
- References
- 4
- FWCI
- 30.12
- Type
- journal article
- Language
- en
- Open access
- green
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- Kim, D. I., Jensen, S. C., Noble, K. A., KC, B., Roux, K. H., Motamedchaboki, K., & Roux, K. J. (2016). An improved smaller biotin ligase for BioID proximity labeling. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e15-12-0844
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