IbMADS1 (Ipomoea batatas MADS-box 1 gene) is Involved in Tuberous Root Initiation in Sweet Potato (Ipomoea batatas)
Open accessAbstract
BACKGROUND AND AIMS: The tuberization mechanism of sweet potato (Ipomoea batatas) has long been studied using various approaches. Morphological data have revealed that the tuberizing events result from the activation of the cambium, followed by cell proliferation. However, uncertainties still remain regarding the regulators participating in this signal-transduction pathway. An attempt was made to characterize the role of one MADS-box transcription factor, which was preferentially expressed in sweet potato roots at the early tuberization stage. METHODS: A differential expression level of IbMADS1 (Ipomoea batatas MADS-box 1) was detected temporally and spatially in sweet potato tissues. IbMADS1 responses to tuberization-related hormones were assessed. In order to identify the evolutionary significance, the expression pattern of IbMADS1 was surveyed in two tuber-deficient Ipomoea relatives, I. leucantha and I. trifida, and compared with sweet potato. In functional analyses, potato (Solanum tuberosum) was employed as a heterologous model. The resulting tuber morphogenesis was examined anatomically in order to address the physiological function of IbMADS1, which should act similarly in sweet potato. KEY RESULTS: IbMADS1 was preferentially expressed as tuberous root development proceeded. Its expression was inducible by tuberization-related hormones, such as jasmonic acid and cytokinins. In situ hybridization data showed that IbMADS1 transcripts were specifically distributed around immature meristematic cells within the stele and lateral root primordia. Inter-species examination indicated that IbMADS1 expression was relatively active in sweet potato roots, but undetectable in tuber-deficient Ipomoea species. IbMADS1-transformed potatoes exhibited tuber morphogenesis in the fibrous roots. The partial swellings along fibrous roots were mainly due to anomalous proliferation and differentiation in the xylem. CONCLUSIONS: Based on this study, it is proposed that IbMADS1 is an important integrator at the initiation of tuberization. As a result, the initiation and development of tuberous roots seems to be well regulated by a network involving a MADS-box gene in which such hormones as jasmonic acid and cytokinins may act as trigger factors.
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ABA stimulates cambial cell division by interacting with CTKs, and this stimulation results in the growth of storage roots.
“Ku et al. [18] reported that ABA stimulates cambial cell division by interacting with CTKs, and this stimulation results in the growth of storage roots.”
From RNA-Seq and iTRAQ reveal multiple pathways involved in storage root formation and development in sweet potato (Ipomoea batatas L.) (2019) · cites this paper as [18]
The Discussion section on hormonal induction associates ABA with cell differentiation in the vascular cambium at later developmental stages, not with stimulating cambial division. It instead describes a synergistic effect of cytokinins and jasmonic acid on the onset of tuberous-root formation. The Results section likewise reports only a weak effect of ABA on IbMADS1 induction. (LLM, conf=0.86)
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Experimental — accuracy not yet measuredIbMADS1 regulates the enlargement of the lateral roots of potato.
“Ku et al. [18] reported that IbMADS1 regulates the enlargement of the lateral roots of potato.”
From RNA-Seq and iTRAQ reveal multiple pathways involved in storage root formation and development in sweet potato (Ipomoea batatas L.) (2019) · cites this paper as [18]
The Results section, “Morphological examination of potato expressing IbMADS1” (page unavailable), reports enlargement and partial swelling of fibrous roots in potatoes engineered to express IbMADS1. It does not identify those swollen roots as lateral roots, and the finding is limited to transgenic potatoes rather than establishing that IbMADS1 regulates lateral-root enlargement in potato generally. (LLM, conf=0.86)
Experimental — accuracy not yet measuredCTKs and JA exert a synergistic effect on the initiation of storage roots and the transcription levels of storage root development-related genes/regulators, such as IbMADS1, sporamin and IbAGPase.
“CTKs and JA exert a synergistic effect on the initiation of storage roots and the transcription levels of storage root development-related genes/regulators, such as IbMADS1, sporamin and IbAGPase [18].”
From RNA-Seq and iTRAQ reveal multiple pathways involved in storage root formation and development in sweet potato (Ipomoea batatas L.) (2019) · cites this paper as [18]
The Results ('Hormone regulation of gene expression and tuberous root formation') report tuber-like swellings after combined JA and cytokinin treatment, but not after either hormone alone at the tested concentrations. IbMADS1 transcripts were abundant and sporamin and IbAGPase were up-regulated in the swollen tissue. The Discussion calls the hormones' effect on tuberization synergistic, but the paper does not establish a synergistic effect on the transcription levels of all three genes. (LLM, conf=0.84)
Experimental — accuracy not yet measuredThe developmental program of storage roots, including the formation of cambium meristems, starch biosynthesis and hormone biosynthesis and transport, mostly relies on transcription factor regulation.
“The developmental program of storage roots, including the formation of cambium meristems, starch biosynthesis and hormone biosynthesis and transport, mostly relies on transcription factor regulation [11, 18].”
From RNA-Seq and iTRAQ reveal multiple pathways involved in storage root formation and development in sweet potato (Ipomoea batatas L.) (2019) · cites this paper as [18]
The Introduction says tuberous-root development involves cambial activity and starch-storage cells, and that such developmental programs mostly rely on transcription-factor regulation. The Results also link IbMADS1 expression with a starch-biosynthesis marker. However, the paper discusses hormones as signals affecting IbMADS1; it does not establish that transcription factors regulate hormone biosynthesis or transport. (LLM, conf=0.86)
Experimental — accuracy not yet measuredThe heterologous overexpression of the sweet potato AGL17 MADS-box gene IbMADS1 in potato causes the fibrous roots of potato to exhibit tuber morphogenesis.
“The heterologous overexpression of the sweet potato AGL17 MADS-box gene IbMADS1 in potato causes the fibrous roots of potato to exhibit tuber morphogenesis [18].”
From RNA-Seq and iTRAQ reveal multiple pathways involved in storage root formation and development in sweet potato (Ipomoea batatas L.) (2019) · cites this paper as [18]
The relationship between stele lignification and inability of adventitious roots to develop into storage roots has been observed by Wilson and Lowe, Belehu et al., and Villordon et al.
“The relationship between stele lignification and inability of adventitious roots to develop into SRs has also been observed by Wilson and Lowe [5], Belehu et al. [6] and Villordon et al. [7].”
From Transcriptional profiling of sweetpotato (Ipomoea batatas) roots indicates down-regulation of lignin biosynthesis and up-regulation of starch biosynthesis at an early stage of storage root formation (2013) · cites this paper as [6]
The Introduction (page unavailable) discusses cambial activity in tuberous-root development, and the Results (page unavailable) describe stele expansion in transgenic potato roots. Neither passage addresses whether stele lignification prevents adventitious roots from becoming storage roots; searches for that relationship and for lignified roots failing to form tuberous roots found no supporting passage. (LLM, conf=0.76)
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References 53 · Cited by 95
Research works printed but not linked: 4
- Artschwager E On the anatomy of sweet potato root with notes on internal breakdown Journal of Agricultural Research 1924 23 157 166
- Gan L-J Zeng X-C Zhou X Possible involvement of jasmonates in the morphogenesis of underground storage organs in plants Chinese Bulletin of Botany 2001 18 546 553
- Sambrook J Russell DW Molecular cloning: a laboratory manual 2001 3rd edn New York Cold Spring Harbor Laboratory Press
- Togari Y A study of tuberous root formation in sweet potato Bulletin of the National Agricultural Exptation Station Tokyo 1950 68 1 96
- Authors
- Ku, A. T., Huang, Y.-S., Wang, Y.-S., Ma, D., & Yeh, K.-W.
- Affiliations
- National Taiwan University (TW)
- Sweet Potato Research Institute (CN)
- Corresponding author
- Yeh, Kai-Wun
- Venue
- Annals of Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-05-07
- Volume
- 102
- Issue
- 1
- Pages
- 57-67
- ISSN
- 0305-7364 (ISSN-L)
- 1095-8290
- PMC
- PMC2712425
- OpenAlex
- W2123360662
- Cited by
- 95
- References
- 53
- Citations by year
- 2025: 5 · 2024: 9 · 2023: 9 · 2022: 8 · 2021: 7 · 2020: 10 · 2019: 7 · 2018: 6 · 2017: 2 · 2016: 8 · 2015: 5 · 2014: 5 · 2013: 4 · 2012: 4
- FWCI
- 2.40
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by
- Locations
- Annals of Botany (publishedVersion, cc-by)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Council
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
- Keywords
- MADS-box transcription factors, tuberization, Ipomoea batatas, jasmonic acid, cytokinins, Ipomoea leucantha, Ipomoea trifida
- MeSH terms
- Abscisic Acid, Cyclopentanes, Cytokinins, Plant Proteins, In Situ Hybridization, Gene Expression Regulation, Plant, Gene Expression Regulation, Developmental, Plant Roots, Reverse Transcriptase Polymerase Chain Reaction, MADS Domain Proteins, Ipomoea batatas, Plants, Genetically Modified, Amplified Fragment Length Polymorphism Analysis, Oxylipins
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