The DOI matches its Crossref record; 181 of the 184 references it cites were found in a registry or an index.
Current challenges and best-practice protocols for microbiome analysis
Open accessAbstract
Analyzing the microbiome of diverse species and environments using next-generation sequencing techniques has significantly enhanced our understanding on metabolic, physiological and ecological roles of environmental microorganisms. However, the analysis of the microbiome is affected by experimental conditions (e.g. sequencing errors and genomic repeats) and computationally intensive and cumbersome downstream analysis (e.g. quality control, assembly, binning and statistical analyses). Moreover, the introduction of new sequencing technologies and protocols led to a flood of new methodologies, which also have an immediate effect on the results of the analyses. The aim of this work is to review the most important workflows for 16S rRNA sequencing and shotgun and long-read metagenomics, as well as to provide best-practice protocols on experimental design, sample processing, sequencing, assembly, binning, annotation and visualization. To simplify and standardize the computational analysis, we provide a set of best-practice workflows for 16S rRNA and metagenomic sequencing data (available at https://github.com/grimmlab/MicrobiomeBestPracticeReview).
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“In contrast to 16S rRNA sequencing, whole-genome shotgun metagenomic sequencing provides more comprehensive taxonomic and functional characterization, encompassing broader genomic content and enabling higher-resolution bacterial community profiling.”
From Microbiome meets immunotherapy: unlocking the hidden predictors of immune checkpoint inhibitors (2025) · cites this paper as [146]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Trimmomatic: a flexible trimmer for Illumina sequence data
Bolger, A. M., Lohse, M., & Usadel, B. (2014) · Bioinformatics · doi:10.1093/bioinformatics/btu170 · Cited by 65,548
Fast and accurate short read alignment with Burrows-Wheeler transform
Li, H., & Durbin, R. (2009) · Bioinformatics · doi:10.1093/bioinformatics/btp324 · Cited by 58,607
Fast gapped-read alignment with Bowtie 2
Langmead, B., & Salzberg, S. L. (2012) · Nature Methods · doi:10.1038/nmeth.1923 · Cited by 56,400
DADA2: High–resolution sample inference from Illumina amplicon data
Callahan, B. J., McMurdie, P. J., Rosen, M. J., Han, A. W., Johnson, A. J. A., & Holmes, S. P. (2016) · Nature Methods · doi:10.1038/nmeth.3869 · Cited by 35,698
Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2
Bolyen, E., Rideout, J. R., Dillon, M. R., Bokulich, N. A., Abnet, C. C., Al-Ghalith, G. A., Alexander, H., Alm, E. J., Arumugam, M., Asnicar, F., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0209-9 · Cited by 23,742
phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data
McMurdie, P. J., & Holmes, S. (2013) · PLoS ONE · doi:10.1371/journal.pone.0061217 · Cited by 21,942
Schloss, P. D., Westcott, S. L., Ryabin, T., Hall, J. R., Hartmann, M., Hollister, E. B., Lesniewski, R. A., Oakley, B. B., Parks, D. H., Robinson, C. J., et al. (2009) · Applied and Environmental Microbiology · doi:10.1128/aem.01541-09 · Cited by 21,481
Prokka: rapid prokaryotic genome annotation
Seemann, T. (2014) · Bioinformatics · doi:10.1093/bioinformatics/btu153 · Cited by 18,937
Fast and sensitive protein alignment using DIAMOND
Buchfink, B., Xie, C., & Huson, D. H. (2015) · Nature Methods · doi:10.1038/nmeth.3176 · Cited by 14,201
Prodigal: prokaryotic gene recognition and translation initiation site identification
Hyatt, D., Chen, G.-L., LoCascio, P. F., Land, M., Larimer, F. W., & Hauser, L. (2010) · BMC Bioinformatics · doi:10.1186/1471-2105-11-119 · Cited by 12,692
Flagged first, then by citations
3 of the 184 references couldn’t be matched to a known paper.
Research works printed but not linked: 3
- Bowtie, 2010, Chapter 11:Unit, 11, 17
- al, 2018, Metagenomics
- Mitra, 2019, Evolutionary Genomics. Methods in Molecular Biology
Cited by
Sorted by citations
Omnipose: a high-precision morphology-independent solution for bacterial cell segmentation
Cutler, K. J., Stringer, C., Lo, T. W., Rappez, L., Stroustrup, N., Peterson, S. B., Wiggins, P. A., & Mougous, J. D. (2022) · Nature Methods · doi:10.1038/s41592-022-01639-4 · Cited by 389
Human microbiome: an academic update on human body site specific surveillance and its possible role
Dekaboruah, E., Suryavanshi, M. V., Chettri, D., & Verma, A. K. (2020) · Archives of Microbiology · doi:10.1007/s00203-020-01931-x · Cited by 361
Open challenges for microbial network construction and analysis
Faust, K. (2021) · The ISME Journal · doi:10.1038/s41396-021-01027-4 · Cited by 316
Current understanding of the Alzheimer’s disease-associated microbiome and therapeutic strategies
Seo, D.-O., & Holtzman, D. M. (2024) · Experimental & Molecular Medicine · doi:10.1038/s12276-023-01146-2 · Cited by 259
Yang, C., Chowdhury, D., Zhang, Z., Cheung, W. K., Lü, A., Bian, Z., & Zhang, L. (2021) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2021.11.028 · Cited by 242
Gut microbiota in overweight and obesity: crosstalk with adipose tissue
Cani, P. D., & Hul, M. V. (2023) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-023-00867-z · Cited by 230
Nanopore sequencing and its application to the study of microbial communities
Ciuffreda, L., Rodríguez‐Pérez, H., & Flores, C. (2021) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2021.02.020 · Cited by 209
Maternal exposures and the infant gut microbiome: a systematic review with meta-analysis
Grech, A. M., Collins, C. E., Holmes, A. J., Lal, R., Duncanson, K., Taylor, R., & Gordon, A. (2021) · Gut Microbes · doi:10.1080/19490976.2021.1897210 · Cited by 198
New Insights Into the Cancer–Microbiome–Immune Axis: Decrypting a Decade of Discoveries
Jain, T., Sharma, P., Are, A. C., Vickers, S. M., & Dudeja, V. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2021.622064 · Cited by 187
Guidelines for preventing and reporting contamination in low-biomass microbiome studies
Fierer, N., Leung, P. M., Lappan, R. J., Eisenhofer, R., Ricci, F., Holland, S. I., Dragone, N. B., Blackall, L. L., Dong, X., Dorador, C., et al. (2025) · Nature Microbiology · doi:10.1038/s41564-025-02035-2 · Cited by 191
Bindari, Y. R., & Gerber, P. F. (2021) · Poultry Science · doi:10.1016/j.psj.2021.101612 · Cited by 162
Rizal, N. S. M., Neoh, H., Ramli, R., Periyasamy, P. R., Hanafiah, A., Samat, M. N. A., Tan, T. L., Wong, K. K., Nathan, S., Chieng, S., et al. (2020) · Diagnostics · doi:10.3390/diagnostics10100816 · Cited by 142
The Association between Vitamin D and Gut Microbiota: A Systematic Review of Human Studies
Bellerba, F., Muzio, V., Gnagnarella, P., Facciotti, F., Chiocca, S., Bossi, P., Cortinovis, D. L., Chiaradonna, F., Serrano, D., Raimondi, S., et al. (2021) · Nutrients · doi:10.3390/nu13103378 · Cited by 137
Safarchi, A., Al-Qadami, G. H., Tran, C. D., & Conlon, M. A. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1559521 · Cited by 145
Regueira‐Iglesias, A., Balsa‐Castro, C., Blanco‐Pintos, T., & Tomás, I. (2023) · Molecular Oral Microbiology · doi:10.1111/omi.12434 · Cited by 146
Kameoka, S., Motooka, D., Watanabe, S., Kubo, R., Jung, N., Midorikawa, Y., Shinozaki, N. O., Sawai, Y., Takeda, A., & Nakamura, S. (2021) · BMC Genomics · doi:10.1186/s12864-021-07746-4 · Cited by 124
Machine Learning and Artificial Intelligence in the Multi-Omics Approach to Gut Microbiota
Rozera, T., Pasolli, E., Segata, N., & Ianiro, G. (2025) · Gastroenterology · doi:10.1053/j.gastro.2025.02.035 · Cited by 112
Plant–Microbe Interaction: Aboveground to Belowground, from the Good to the Bad
Nadarajah, K. K., & Rahman, N. S. N. A. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms221910388 · Cited by 114
Untapping the potential of plant mycobiomes for applications in agriculture
Pozo, M. J., Zabalgogeazcoa, Í., de Aldana, B. R. V., & Martínez‐Medina, A. (2021) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2021.102034 · Cited by 105
The emerging role of the gut microbiota and its application in inflammatory bowel disease
Xiu, W., Peng, J., Cai, P., Xia, Y., Yi, C., Shang, A., Akanyibah, F. A., & Mao, F. (2024) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2024.117302 · Cited by 117
Yang, S.-Y., Han, S. M., Lee, J., Kim, K. S., Lee, J. E., & Lee, D. (2025) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.2412.12001 · Cited by 124
Unraveling the Gut Microbiota: Implications for Precision Nutrition and Personalized Medicine
Abeltino, A., Hatem, D., Serantoni, C., Riente, A., Giulio, M. M. D., Spirito, M. D., Maio, F. D., & Maulucci, G. (2024) · Nutrients · doi:10.3390/nu16223806 · Cited by 121
Data Analytics for Environmental Science and Engineering Research
Gupta, S., Aga, D. S., Pruden, A. J., Zhang, L., & Vikesland, P. J. (2021) · Environmental Science & Technology · doi:10.1021/acs.est.1c01026 · Cited by 100
Rashidah, N. H., Lim, S. M., Neoh, C. F., Majeed, A. B. A., Tan, M. P., Khor, H. M., Tan, A. H., Rehiman, S. H., & Ramasamy, K. (2022) · Ageing Research Reviews · doi:10.1016/j.arr.2022.101744 · Cited by 100
Li, S., Lian, W., Han, J.-R., Ali, M., Lin, Z., Liu, Y., Li, L., Zhang, D., Jiang, X., Li, W., et al. (2023) · npj Biofilms and Microbiomes · doi:10.1038/s41522-023-00439-8 · Cited by 95
Current Methods, Common Practices, and Perspectives in Tracking and Monitoring Bioinoculants in Soil
Manfredini, A., Malusà, E., Costa, C., Pallottino, F., Mocali, S., Pinzari, F., & Canfora, L. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.698491 · Cited by 95
Gut microbiota and metabolic biomarkers in metabolic dysfunction–associated steatotic liver disease
Long, Q., Luo, F., Li, B., Li, Z., Guo, Z., Chen, Z., Wu, W., & Hu, M. (2024) · Hepatology Communications · doi:10.1097/hc9.0000000000000310 · Cited by 99
Gut microbiota development across the lifespan: Disease links and health‐promoting interventions
Schoultz, I., Claesson, M. J., Domínguez-Bello, M. G., Hållenius, F. F., Konturek, P. C., Korpela, K., Laursen, M. F., Penders, J., Roager, H. M., Vatanen, T., et al. (2025) · Journal of Internal Medicine · doi:10.1111/joim.20089 · Cited by 108
Experimental temperatures shape host microbiome diversity and composition
Li, J., Bates, K. A., Hoang, K. L., Hector, T. E., Knowles, S. C. L., & King, K. C. (2022) · Global Change Biology · doi:10.1111/gcb.16429 · Cited by 101
The Human Respiratory Microbiome: Current Understandings and Future Directions
Zhao, L., Luo, J., Ali, M. K., Spiekerkoetter, E. F., & Nicolls, M. R. (2022) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2022-0208tr · Cited by 96
Lin, A., Xiong, M., Jiang, A., Huang, L., Wong, H. Z. H., Feng, S., Zhang, C., Yu, H., Chen, L., Chi, H., et al. (2025) · iMeta · doi:10.1002/imt2.70070 · Cited by 99
Davis, B. C., Brown, C., Gupta, S., Calarco, J., Liguori, K., Milligan, E., Harwood, V. J., Pruden, A. J., & Keenum, I. M. (2023) · Critical Reviews in Environmental Science and Technology · doi:10.1080/10643389.2023.2181620 · Cited by 83
In situ identification of environmental microorganisms with Raman spectroscopy
Cui, D., Kong, L., Wang, Y., Zhu, Y., & Zhang, C. (2022) · Environmental Science and Ecotechnology · doi:10.1016/j.ese.2022.100187 · Cited by 90
So, D., Loughman, A., & Staudacher, H. M. (2022) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/nqac176 · Cited by 85
Jang, D., Shin, J., Shim, E., Ohtani, N., & Jeon, O. H. (2024) · Aging Cell · doi:10.1111/acel.14315 · Cited by 84
Wu, J., Singleton, S. S., Bhuiyan, U., Krammer, L., & Mazumder, R. (2024) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2023.1337373 · Cited by 85
The role of the microbiome in gastrointestinal inflammation
Sanders, D. J., Inniss, S., Sebepos‐Rogers, G., Rahman, F. Z., & Smith, A. M. (2021) · Bioscience Reports · doi:10.1042/bsr20203850 · Cited by 82
Kullar, R., Chisari, E., Snyder, J. W., Cooper, C., Parvizi, J., & Sniffen, J. C. (2022) · Clinical Infectious Diseases · doi:10.1093/cid/ciac733 · Cited by 76
Genome-Scale Metabolic Modeling of the Human Microbiome in the Era of Personalized Medicine
Heinken, A., Basile, A., Hertel, J., Thinnes, C. C., & Thiele, I. (2021) · Annual Review of Microbiology · doi:10.1146/annurev-micro-060221-012134 · Cited by 76
Artificial intelligence and synthetic biology approaches for human gut microbiome
Kumar, P., Sinha, R., & Shukla, P. (2020) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2020.1850415 · Cited by 78
Charting the complexity of the activated sludge microbiome through a hybrid sequencing strategy
Liu, L., Wang, Y., Yu, Y., Wang, D., Cheng, S. H., Zheng, C., & Zhang, T. (2021) · Microbiome · doi:10.1186/s40168-021-01155-1 · Cited by 67
coda4microbiome: compositional data analysis for microbiome cross-sectional and longitudinal studies
Calle, M. L., Pujolassos, M., & Susín, A. (2023) · BMC Bioinformatics · doi:10.1186/s12859-023-05205-3 · Cited by 74
Penumutchu, S., Korry, B. J., Hewlett, K. K., & Belenky, P. A. (2023) · Nature Communications · doi:10.1038/s41467-023-40553-x · Cited by 73
A review of gut failure as a cause and consequence of critical illness
Soranno, D. E., Coopersmith, C. M., Brinkworth, J. F., Factora, F. N., Muntean, J. H., Mythen, M. G., Raphael, J., Shaw, A., Vachharajani, V., & Messer, J. S. (2025) · Critical Care · doi:10.1186/s13054-025-05309-7 · Cited by 77
Application of Microbiome in Forensics
Zhang, J., Liu, W., Simayijiang, H., Hu, P., & Yan, J. (2022) · Genomics Proteomics & Bioinformatics · doi:10.1016/j.gpb.2022.07.007 · Cited by 75
Wani, A. K., Akhtar, N., Naqash, N., Rahayu, F., Djajadi, D., Chopra, C., Singh, R., Mulla, S. I., Sher, F., & Américo‐Pinheiro, J. H. P. (2023) · Environmental Science and Pollution Research · doi:10.1007/s11356-023-25192-5 · Cited by 68
Cullen, J. M. A., Shahzad, S., & Dhillon, J. (2023) · Frontiers in Physiology · doi:10.3389/fphys.2023.1292673 · Cited by 76
Recent advances in bivalve-microbiota interactions for disease prevention in aquaculture
Christine Paillard, A., Gueguen, Y., Wegner, K. M., Bass, D., Pallavicini, A., Vezzulli, L., & Arzul, I. (2021) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2021.07.026 · Cited by 66
Tousoulis, D., Guzik, T. J., Padró, T., Duncker, D. J., Luca, G. D., Eringa, E. C., Vavlukis, M., Antonopoulos, A. S., Katsimichas, T., Cenko, E., et al. (2022) · Cardiovascular Research · doi:10.1093/cvr/cvac057 · Cited by 67
Bastiaanssen, T. F. S., Quinn, T. P., & Loughman, A. (2023) · Nature Mental Health · doi:10.1038/s44220-023-00148-3 · Cited by 71
The intratumoral microbiome: Characterization methods and functional impact
Heymann, C. J. F., Bard, J., Heymann, M., Heymann, D., & Bobin‐Dubigeon, C. (2021) · Cancer Letters · doi:10.1016/j.canlet.2021.09.009 · Cited by 60
Phage-inclusive profiling of human gut microbiomes with Phanta
Pinto, Y., Chakraborty, M., Jain, N., & Bhatt, A. S. (2023) · Nature Biotechnology · doi:10.1038/s41587-023-01799-4 · Cited by 57
Imran, M., Feng, X., Sun, Z., AlOmari, H., Zhang, G., Zhu, J., Aldayel, M. F., & Li, C. (2025) · Chemical and Biological Technologies in Agriculture · doi:10.1186/s40538-025-00738-6 · Cited by 62
Phage therapeutics: from promises to practices and prospectives
Bhargava, K., Nath, G., Bhargava, A., Aseri, G. K., & Jain, N. (2021) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-021-11695-z · Cited by 58
Dreier, M., Meola, M., Berthoud, H., Shani, N., Wechsler, D., & Junier, P. (2022) · BMC Microbiology · doi:10.1186/s12866-022-02451-y · Cited by 58
Type 2 diabetes, gut microbiome, and systems biology: A novel perspective for a new era
Martínez-López, Y. E., Esquivel-Hernandez, D. A., Sánchez-Castañeda, J. P., Neri-Rosario, D., Guardado‐Mendoza, R., & Reséndis-Antonio, O. (2022) · Gut Microbes · doi:10.1080/19490976.2022.2111952 · Cited by 59
Gut Microbiome Analysis for Personalized Nutrition: The State of Science
Simon, M., Sina, C., Ferrario, P. G., Daniel, H., & Working Group “Personalized Nutrition” of the German Nutrition Society (2022) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.202200476 · Cited by 60
Cutting edge tools in the field of soil microbiology
Garg, D., Patel, N. S., Rawat, A., & Rosado, A. S. (2024) · Current Research in Microbial Sciences · doi:10.1016/j.crmicr.2024.100226 · Cited by 56
Role of the duodenal microbiota in functional dyspepsia
Brown, G., Hoedt, E. C., Keely, S., Shah, A., Walker, M. M., Holtmann, G., & Talley, N. J. (2022) · Neurogastroenterology & Motility · doi:10.1111/nmo.14372 · Cited by 58
The Intestinal Microbiota May Be a Potential Theranostic Tool for Personalized Medicine
Domenico, M. D., Ballini, A., Boccellino, M., Scacco, S., Lovero, R., Charitos, I. A., & Santacroce, L. (2022) · Journal of Personalized Medicine · doi:10.3390/jpm12040523 · Cited by 54
Shifts of microbiota during cheese production: Impact on production and quality
Nam, J. H., Cho, Y. S., Rackerby, B., Goddik, L., & Park, S. H. (2021) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-021-11201-5 · Cited by 53
Considerations for mosquito microbiome research from the Mosquito Microbiome Consortium
Dada, N., Jupatanakul, N., Minard, G., Short, S. M., Akorli, J., & Villegas, L. E. M. (2021) · Microbiome · doi:10.1186/s40168-020-00987-7 · Cited by 54
Tegegne, H. A., & Savidge, T. (2025) · Gut Microbes · doi:10.1080/19490976.2025.2569739 · Cited by 59
Microbiome in Death and Beyond: Current Vistas and Future Trends
Roy, D., Tomo, S., Purohit, P., & Setia, P. (2021) · Frontiers in Ecology and Evolution · doi:10.3389/fevo.2021.630397 · Cited by 55
Slanzon, G. S., Ridenhour, B. J., Moore, D. A., Sischo, W. M., Parrish, L. M., Trombetta, S. C., & McConnel, C. S. (2022) · PLoS ONE · doi:10.1371/journal.pone.0262317 · Cited by 49
Gut Microbiota and Microbial Metabolism in Early Risk of Cardiometabolic Disease
Gabriel, C. L., & Ferguson, J. F. (2023) · Circulation Research · doi:10.1161/circresaha.123.322055 · Cited by 51
HoCoRT: host contamination removal tool
Rumbavicius, I., Rounge, T. B., & Rognes, T. (2023) · BMC Bioinformatics · doi:10.1186/s12859-023-05492-w · Cited by 46
Imanian, B., Donaghy, J., Jackson, T. C., Gummalla, S., Ganesan, B., Baker, R. C., Henderson, M., Butler, E. K., Hong, Y., Ring, B., et al. (2022) · npj Science of Food · doi:10.1038/s41538-022-00150-6 · Cited by 46
Unravelling the enigma of the human microbiome: Evolution and selection of sequencing technologies
Xin, Y., Lu, H., Liu, X., He, J., Li, B. J., Wang, Z., Zhao, Y., Zhang, X., & Yu, X. (2023) · Microbial Biotechnology · doi:10.1111/1751-7915.14364 · Cited by 47
Lista, S., Munafò, A., Caraci, F., Imbimbo, C., Emanuele, E., Minoretti, P., Pinto-Fraga, J., Merino-País, M., Crespo‐Escobar, P., López‐Ortiz, S., et al. (2025) · Ageing Research Reviews · doi:10.1016/j.arr.2025.102659 · Cited by 50
Reconstruction of the personal information from human genome reads in gut metagenome sequencing data
Tomofuji, Y., Sonehara, K., Kishikawa, T., Maeda, Y., Ogawa, K., Kawabata, S., Nii, T., Okuno, T., Oguro-Igashira, E., Kinoshita, M., et al. (2023) · Nature Microbiology · doi:10.1038/s41564-023-01381-3 · Cited by 45
Gut microbiome and amyotrophic lateral sclerosis: a systematic review of current evidence
Sun, J., Huang, T., Debelius, J. W., & Fang, F. (2021) · Journal of Internal Medicine · doi:10.1111/joim.13336 · Cited by 43
Behera, B. K., Dehury, B., Rout, A. K., Patra, B., Mantri, N. L., Chakraborty, H. J., Sarkar, D. J., Kaushik, N. K., Bansal, V., Singh, I., et al. (2021) · Gene Reports · doi:10.1016/j.genrep.2021.101372 · Cited by 45
Sung, C., Pilla, R., Chen, C.-C., Ishii, P. E., Toresson, L., Allenspach‐Jorn, K., Jergens, A. E., Summers, S., Swanson, K. S., Volk, H. A., et al. (2023) · Animals · doi:10.3390/ani13162597 · Cited by 52
Kaur, H., Kaur, G., Gupta, T., Mittal, D., & Ali, S. A. (2023) · Biology · doi:10.3390/biology12091200 · Cited by 47
From hype to hope: Considerations in conducting robust microbiome science
McGuinness, A. J., Stinson, L. F., Snelson, M., Loughman, A., Stringer, A. M., Hannan, A. J., Cowan, C. S. M., Jama, H. A., Caparros-Martin, J. A., West, M. L., et al. (2023) · Brain Behavior and Immunity · doi:10.1016/j.bbi.2023.09.022 · Cited by 44
Evidence for the contribution of the gut microbiome to obesity and its reversal
de Wit, D. F., Hanssen, N. M. J., Wortelboer, K., Herrema, H., Rampanelli, E., & Nieuwdorp, M. (2023) · Science Translational Medicine · doi:10.1126/scitranslmed.adg2773 · Cited by 50
Unlocking the Potential of Metagenomics with the PacBio High-Fidelity Sequencing Technology
Han, Y., He, J., Li, M., Peng, Y., Jiang, H., Zhao, J., Li, Y., & Deng, F. (2024) · Microorganisms · doi:10.3390/microorganisms12122482 · Cited by 43
Vaginal microbiome of women with adenomyosis: A case-control study
Kunaseth, J., Waiyaput, W., Chanchaem, P., Sawaswong, V., Permpech, R., Payungporn, S., & Sophonsritsuk, A. (2022) · PLoS ONE · doi:10.1371/journal.pone.0263283 · Cited by 41
The Microbiome as a Maternal Effect: A Systematic Review on Vertical Transmission of Microbiota
Murphy, K., Le, S. M., Wilson, A. E., & Warner, D. A. (2023) · Integrative and Comparative Biology · doi:10.1093/icb/icad031 · Cited by 40
Romanis, C. S., Pearson, L. A., & Neilan, B. A. (2020) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2020.106123 · Cited by 43
Benchmarking genome assembly methods on metagenomic sequencing data
Zhang, Z., Yang, C., Veldsman, W. P., Fang, X., & Zhang, L. (2023) · Briefings in Bioinformatics · doi:10.1093/bib/bbad087 · Cited by 39
Application of emerging technologies for gut microbiome research
Kwa, W. T., Sundarajoo, S., Toh, K. Y., & Lee, J. W. J. (2023) · Singapore Medical Journal · doi:10.4103/singaporemedj.smj-2021-432 · Cited by 40
Palmieri, O., Castellana, S., Biscaglia, G., Panza, A., Latiano, A., Fontana, R., Guerra, M., Corritore, G., Latiano, T. P., Martino, G., et al. (2021) · Cells · doi:10.3390/cells10113243 · Cited by 37
Nine (not so simple) steps: a practical guide to using machine learning in microbial ecology
Walsh, C. M., Stallard-Olivera, E., & Fierer, N. (2023) · mBio · doi:10.1128/mbio.02050-23 · Cited by 41
Malakar, S., Sutaoney, P., Madhyastha, H., Shah, K., Chauhan, N. S., & Banerjee, P. (2024) · Chemical Biology & Drug Design · doi:10.1111/cbdd.14505 · Cited by 37
Fan, K.-C., Lin, C.-C., Chiu, Y., Koh, S., Liu, Y., & Chuang, Y. (2025) · Alzheimer s Research & Therapy · doi:10.1186/s13195-025-01769-9 · Cited by 42
Djemiel, C., Goulas, E., Badalato, N., Chabbert, B., Hawkins, S., & Grec, S. (2020) · Frontiers in Genetics · doi:10.3389/fgene.2020.581664 · Cited by 42
Rieder, J., Kapopoulou, A., Bank, C., & Adrian‐Kalchhauser, I. (2023) · Environmental Microbiome · doi:10.1186/s40793-023-00459-z · Cited by 50
Notario, E., Visci, G., Fosso, B., Gissi, C., Tanasković, N., Rescigno, M., Marzano, M., & Pesole, G. (2023) · Genes · doi:10.3390/genes14081567 · Cited by 38
Ansorge, R., Birolo, G., James, S. A., & Telatin, A. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22105309 · Cited by 36
Lathe, R., Schultek, N. M., Balin, B. J., Ehrlich, G. D., Alberi, L. A., Perry, G. L., Breitschwerdt, E. B., Corry, D. B., Doty, R. L., Rissman, R. A., et al. (2023) · Alzheimer s & Dementia · doi:10.1002/alz.13076 · Cited by 39
Lee, Y., Cappellato, M., & Camillo, B. D. (2022) · GigaScience · doi:10.1093/gigascience/giad083 · Cited by 36
A Perspective on the Role of Microbiome for Colorectal Cancer Treatment
Kalasabail, S., Engelman, J., Zhang, L. Y., El-Omar, E. M., & Yim, H. C. H. (2021) · Cancers · doi:10.3390/cancers13184623 · Cited by 36
Long-Reads-Based Metagenomics in Clinical Diagnosis With a Special Focus on Fungal Infections
Hoang, M. T. V., Irinyi, L., Hu, Y., Schwessinger, B., & Meyer, W. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.708550 · Cited by 35
Rochegüe, T., Haenni, M., Mondot, S., Astruc, C., Cazeau, G., Ferry, T., Madec, J.-Y., & Lupo, A. (2021) · Animals · doi:10.3390/ani11113280 · Cited by 34
Rangel-Piñeros, G., Almeida, A., Beracochea, M., Sakharova, E., Marz, M., Reyes, A., Hölzer, M., & Finn, R. D. (2023) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1011422 · Cited by 34
Mannion, A. J., Sheh, A., Shen, Z., Dzink-Fox, J., Piazuelo, M. B., Wilson, K. T., Peek, R. M., & Fox, J. G. (2023) · Gut Microbes · doi:10.1080/19490976.2023.2186677 · Cited by 33
Seibert, B., Cáceres, C. J., Garcia, S. C., Carnaccini, S., Geiger, G., Rajão, D. S., Ottesen, E. A., & Perez, D. R. (2021) · Microbiology Spectrum · doi:10.1128/spectrum.00536-21 · Cited by 33
Microbiome Research and Multi-Omics Integration for Personalized Medicine in Asthma
Logotheti, M., Agioutantis, P. C., Katsaounou, P. A., & Loutrari, H. V. (2021) · Journal of Personalized Medicine · doi:10.3390/jpm11121299 · Cited by 32
Metadata
- Authors
- Bharti, R., & Grimm, D. G.
- Affiliations
- Weihenstephan-Triesdorf University of Applied Sciences (DE)
- Corresponding author
- Grimm, Dominik G
- Venue
- Briefings in Bioinformatics
- Publisher
- Oxford University Press (OUP)
- Published
- 2019-12-18
- Volume
- 22
- Issue
- 1
- Pages
- 178-193
- ISSN
- 1467-5463 (ISSN-L)
- 1477-4054
- PMC
- PMC7820839
- OpenAlex
- W2995649573
- Cited by
- 592
- References
- 184
- Citations by year
- 2026: 91 · 2025: 129 · 2024: 101 · 2023: 107 · 2022: 80 · 2021: 74 · 2020: 10
- FWCI
- 3.61
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Briefings in Bioinformatics (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich) (submittedVersion)
- mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- microbiome analysis, 16S rRNA gene sequencing, shotgun metagenomics, long-read metagenomics, best practice protocol, sample processing, sequencing quality control, metagenome assembly, metagenome binning, functional annotation, data visualization, experimental design
- MeSH terms
- Animals, Humans, RNA, Ribosomal, 16S, Practice Guidelines as Topic, Sequence Analysis, DNA, Metagenomics, DNA Barcoding, Taxonomic, Microbiota
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review