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Bismark: a flexible aligner and methylation caller for Bisulfite-Seq applications

Open access
Krueger, Felix, Andrews, Simon R.·2011·Bioinformatics

Abstract

Summary: A combination of bisulfite treatment of DNA and high-throughput sequencing (BS-Seq) can capture a snapshot of a cell's epigenomic state by revealing its genome-wide cytosine methylation at single base resolution. Bismark is a flexible tool for the time-efficient analysis of BS-Seq data which performs both read mapping and methylation calling in a single convenient step. Its output discriminates between cytosines in CpG, CHG and CHH context and enables bench scientists to visualize and interpret their methylation data soon after the sequencing run is completed. Availability and implementation: Bismark is released under the GNU GPLv3+ licence. The source code is freely available from www.bioinformatics.bbsrc.ac.uk/projects/bismark/. Contact: felix.krueger@bbsrc.ac.uk Supplementary information: Supplementary data are available at Bioinformatics online.

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Research works printed but not linked: 6

  1. Chen, 2010, BS Seeker: precise mapping for bisulfite sequencing, BMC Bioinformatics, 11, 203, 10.1186/1471-2105-11-203 · DOI · Paper page
  2. Cokus, 2008, Shotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterning, Nature, 452, 215, 10.1038/nature06745 · DOI · Paper page
  3. Harris, 2010, Comparison of sequencing-based methods to profile DNA methylation and identification of monoallelic epigenetic modifications, Nat. Biotechnol., 28, 1097, 10.1038/nbt.1682 · DOI · Paper page
  4. Law, 2010, Establishing, maintaining and modifying DNA methylation patterns in plants and animals, Nat. Rev. Genet., 11, 204, 10.1038/nrg2719 · DOI · Paper page
  5. Popp, 2010, Genome-wide erasure of DNA methylation in mouse primordial germ cells is affected by AID deficiency, Nature, 463, 1101, 10.1038/nature08829 · DOI · Paper page
  6. Robertson, 2005, DNA methylation and human disease, Nat. Rev. Genet., 6, 597, 10.1038/nrg1655 · DOI · Paper page

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Metadata

Authors
Krueger, F., & Andrews, S. R.
Venue
Bioinformatics
Published
2011-04-14
Volume
27
Issue
11
Pages
1571-1572
ISSN
  • 1367-4803 (ISSN-L)
  • 1367-4811
OpenAlex
W2131374955
Cited by
5,297
References
3
FWCI
25.51
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Bismark: a flexible aligner and methylation caller for Bisulfite-Seq applications. https://app.cochat.ai/papers/10.1093/bioinformatics/btr167
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Krueger, F., & Andrews, S. R. (2011). Bismark: a flexible aligner and methylation caller for Bisulfite-Seq applications. Bioinformatics. https://doi.org/10.1093/bioinformatics/btr167

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