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The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.

Identification of novel transcripts in annotated genomes using RNA-Seq

Open access
Roberts, Adam, Pimentel, Harold, Trapnell, Cole, Pachter, Lior·2011·Bioinformatics

Abstract

Summary: We describe a new ‘reference annotation based transcript assembly’ problem for RNA-Seq data that involves assembling novel transcripts in the context of an existing annotation. This problem arises in the analysis of expression in model organisms, where it is desirable to leverage existing annotations for discovering novel transcripts. We present an algorithm for reference annotation-based transcript assembly and show how it can be used to rapidly investigate novel transcripts revealed by RNA-Seq in comparison with a reference annotation. Availability: The methods described in this article are implemented in the Cufflinks suite of software for RNA-Seq, freely available from http://bio.math.berkeley.edu/cufflinks. The software is released under the BOOST license. Contact: cole@broadinstitute.org; lpachter@math.berkeley.edu Supplementary Information: Supplementary data are available at Bioinformatics online.

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Metadata

Authors
Roberts, A., Pimentel, H., Trapnell, C., & Pachter, L.
Venue
Bioinformatics
Published
2011-06-21
Volume
27
Issue
17
Pages
2325-2329
ISSN
  • 1367-4803 (ISSN-L)
  • 1367-4811
OpenAlex
W2098832713
Cited by
972
References
4
FWCI
27.39
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Identification of novel transcripts in annotated genomes using RNA-Seq. https://app.cochat.ai/papers/10.1093/bioinformatics/btr355
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Roberts, A., Pimentel, H., Trapnell, C., & Pachter, L. (2011). Identification of novel transcripts in annotated genomes using RNA-Seq. Bioinformatics. https://doi.org/10.1093/bioinformatics/btr355

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