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PoPoolation2: identifying differentiation between populations using sequencing of pooled DNA samples (Pool-Seq)
Open accessAbstract
SUMMARY: Sequencing pooled DNA samples (Pool-Seq) is the most cost-effective approach for the genome-wide comparison of population samples. Here, we introduce PoPoolation2, the first software tool specifically designed for the comparison of populations with Pool-Seq data. PoPoolation2 implements a range of commonly used measures of differentiation (F(ST), Fisher's exact test and Cochran-Mantel-Haenszel test) that can be applied on different scales (windows, genes, exons, SNPs). The result may be visualized with the widely used Integrated Genomics Viewer. AVAILABILITY AND IMPLEMENTATION: PoPoolation2 is implemented in Perl and R. It is freely available on http://code.google.com/p/popoolation2/ CONTACT: christian.schloetterer@vetmeduni.ac.at SUPPLEMENTARY INFORMATION: Manual: http://code.google.com/p/popoolation2/wiki/Manual Test data and tutorial: http://code.google.com/p/popoolation2/wiki/Tutorial Validation: http://code.google.com/p/popoolation2/wiki/Validation.
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“Variants were finally filtered using PoPoolation2 (80) to yield a high-confidence set of SNVs (n = 6,534,957; filtering steps included a minimum coverage of 8, a minimum variant count of 6, a maximum coverage of 500 for the domestic cat pool, and a maximum coverage of 200 for the wildcat pool).”
From Comparative analysis of the domestic cat genome reveals genetic signatures underlying feline biology and domestication (2014) · cites this paper as [80]
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Metadata
- Authors
- Kofler, R., Pandey, R. V., & Schlötterer, C.
- Affiliations
- University of Veterinary Medicine Vienna (AT)
- Corresponding author
- Schlötterer, Christian
- Venue
- Bioinformatics
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-10-23
- Volume
- 27
- Issue
- 24
- Pages
- 3435-3436
- ISSN
- 1367-4803 (ISSN-L)
- 1367-4811
- PMC
- PMC3232374
- OpenAlex
- W2161950199
- Cited by
- 815
- References
- 23
- Citations by year
- 2026: 44 · 2025: 48 · 2024: 68 · 2023: 54 · 2022: 86 · 2021: 83 · 2020: 88 · 2019: 77 · 2018: 64 · 2017: 54 · 2016: 62 · 2015: 34 · 2014: 22 · 2013: 26 · 2012: 2
- FWCI
- 9.98
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Bioinformatics (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- Austrian Science Fund (P 22725, P19467)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
- Keywords
- pooled DNA sequencing, genetic differentiation, F(ST), Fisher's exact test, Cochran-Mantel-Haenszel test, population genetics software
- MeSH terms
- Animals, Drosophila melanogaster, Genetics, Population, Software, Arabidopsis, Sequence Analysis, DNA, Polymorphism, Single Nucleotide, Genomics
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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