Expression of Jun family members in human colorectal adenocarcinoma
Abstract
The products of the Jun family genes, c-Jun, JunB and JunD, are essential components of the activating protein-1 transcription factor complexes that are critically important in the control of cell growth, differentiation and neoplastic transformation. Although increased c-Jun expression has been reported in human colorectal tumors, expression of JunB and JunD in these tumors has not previously been characterized. In the current study, we examined 24 cases of human colorectal adenocarcinoma by western immunoblotting analysis and immunohistochemical staining for the expression of c-Jun, JunB and JunD proteins. Normal-appearing colonic mucosa distant from the tumors in the same colectomy specimens were used as a reference for comparison. The results showed that both c-Jun and JunB proteins were undetectable or barely detectable in normal mucosa but their expression levels were significantly increased in human colorectal adenocarcinomas. In contrast, JunD protein was present at high levels in normal mucosa and only showed a minimal increase in adenocarcinomas. These observations suggest that different Jun proteins may serve different roles in regulating colonic epithelial cell growth and in colorectal tumorigenesis.
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Different studies in several human neoplasms, including CRC, showed higher c-JUN expression and activation in tumors compared to normal tissue.
“Different studies in several human neoplasms [50–53], including CRC [54], showed higher c-JUN expression and activation in tumors compared to normal tissue.”
From Co-targeting SRC overcomes resistance to BRAF inhibitors in colorectal cancer (2025) · cites this paper as [54]
The Results report higher c-Jun protein expression in 24 human colorectal adenocarcinomas than in paired normal mucosa. The paper measures expression, not c-Jun activation in those tumors, so it does not support that part of the claim. The other neoplasms are attributed to different citations. (LLM, conf=0.85)
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Co-targeting SRC overcomes resistance to BRAF inhibitors in colorectal cancer
Rubio-Cuesta, B., Carretero-Puche, C., Llamas, P., Sarmentero, J., Gil-Calderon, B., Lens-Pardo, A., Antón-Pascual, B., Rubio-González, E., Cámara-Jurado, M., Salamanca, J., et al. (2025) · British Journal of Cancer · doi:10.1038/s41416-025-03058-6 · Cited by 7 · see citing works
- Authors
- Wang, H., Birkenbach, M., & Hart, J.
- Affiliations
- University of Chicago Medical Center (US)
- Venue
- Carcinogenesis
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-07
- Volume
- 21
- Issue
- 7
- Pages
- 1313-1317
- ISSN
- 0143-3334 (ISSN-L)
- 1460-2180
- OpenAlex
- W2107212237
- Cited by
- 64
- References
- 21
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 1 · 2023: 7 · 2022: 3 · 2021: 3 · 2020: 1 · 2019: 4 · 2018: 1 · 2017: 2 · 2016: 4 · 2015: 3 · 2014: 1 · 2013: 5 · 2012: 1
- FWCI
- 1.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Carcinogenesis (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Chicago
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Cancer Mechanisms and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Digestive system and related health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
- Keywords
- c-Jun, JunB, JunD, colorectal adenocarcinoma, activating protein-1, protein expression, colonic mucosa, colorectal carcinogenesis
- MeSH terms
- Adenocarcinoma, Humans, Immunohistochemistry, Intestinal Mucosa, Neoplasm Staging, Blotting, Western, Colorectal Neoplasms, Up-Regulation, Proto-Oncogene Proteins c-jun
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