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A New and Rapid Method for the Determination of Glucose by Measurement of Rate of Oxygen Consumption
Abstract
Glucose levels in serum, plasma, and urine are determined rapidly and conveniently by a new glucose oxidase method employing a polarographic oxygen sensor with a circuit modified to record the rate of oxygen consumption. The maximum apparent rate of oxygen consumption relative to the rate obtained with a glucose standard provides a direct measure of the glucose level in the sample; results are obtainable within 20 sec. after sample (100 µl.) addition and within 3 min. after a blood sample is withdrawn from a patient. Interferences associated with prior colorimetric glucose oxidase methods are avoided by measuring oxygen consumption instead of hydrogen peroxide formation. The method is described and results are presented showing a standard deviation of less than 1.5% on replicate determinations and a bias of 1% with respect to data obtained on the same samples by the automated ferricyanide method.
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Citations
Cited by
Electrochemical Glucose Sensors and Their Applications in Diabetes Management
Heller, A., & Feldman, B. (2008) · Chemical Reviews · doi:10.1021/cr068069y · Cited by 1,707
Martin, B. C., Warram, J. H., Krolewski, A. S., Soeldner, J. S., Kahn, C. R., Martin, B. C., & Bergman, R. N. (1992) · The Lancet · doi:10.1016/0140-6736(92)92814-v · Cited by 1,158
Seppala-Lindroos, A. (2002) · Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.87.7.3023 · Cited by 168
Warram, J. H., Martin, B. C., Krolewski, A. S., Soeldner, J. S., & Kahn, C. R. (1990) · Annals of Internal Medicine · doi:10.7326/0003-4819-113-12-909 · Cited by 1,038
Use of metabolic markers to identify overweight individuals who are insulin resistant
McLaughlin, T., Abbasi, F., Cheal, K., Chu, J., Lamendola, C., & Reaven, G. (2003) · Annals of Internal Medicine · doi:10.7326/0003-4819-139-10-200311180-00007 · Cited by 1,022
Metadata
- Authors
- Kadish, A. H., Litle, R. L., & Sternberg, J. C.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1968-02-01
- Volume
- 14
- Issue
- 2
- Pages
- 116-131
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2112538182
- Cited by
- 740
- References
- 0
- FWCI
- 9.67
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kadish, A. H., Litle, R. L., & Sternberg, J. C. (1968). A New and Rapid Method for the Determination of Glucose by Measurement of Rate of Oxygen Consumption. Clinical Chemistry. https://doi.org/10.1093/clinchem/14.2.116
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
