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Sodium Thymolphthalein Monophosphate: A New Acid Phosphatase Substrate with Greater Specificity for the Prostatic Enzyme in Serum
Abstract
We describe the use of sodium thymolphthalein monophosphate as a substrate for measuring acid phosphatase activity in serum. Thymolphthalein is liberated in the reaction and is conveniently measured by increasing the pH of the medium, which produces a color and also stops the hydrolysis. Optimal conditions for enzymatic activity were defined for use in the final assay system. Purified acid phosphatase isoenzymes from various human tissues and sera from patients with nonprostatic diseases most likely to affect serum acid phosphatase activity were used to show that the new method is more specific for the prostatic enzyme than other commonly used procedures for acid phosphatase. Other advantages include simplicity, good sensitivity over a wide range of activity, high precision, zero-order kinetics to about 200 times the upper limit of normal, easy standardization by a pure standard, and reagent stability.
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Citations
Cited by
Clinical significance of the human acid phosphatases
Yam, L. T. (1974) · The American Journal of Medicine · doi:10.1016/0002-9343(74)90630-5 · Cited by 292
Bicalutamide for advanced prostate cancer: the natural versus treated history of disease.
Scher, H. I., Liebertz, C., Kelly, W. K., Mazumdar, M., Brett, C., Schwartz, L., Kolvenbag, G., Shapiro, L., & Schwartz, M. (1997) · Journal of Clinical Oncology · doi:10.1200/jco.1997.15.8.2928 · Cited by 227
In vivo biocompatibility studies. I. The cage implant system and a biodegradable hydrogel
Marchant, R., Hiltner, A., Hamlin, C., Rabinovitch, A., Slobodkin, R., & Anderson, J. M. (1983) · Journal of Biomedical Materials Research · doi:10.1002/jbm.820170209 · Cited by 181
Assessment of environmental factors affecting male fertility
Dixon, R. L., Sherins, R. J., & Lee, I. P. (1979) · Environmental Health Perspectives · doi:10.1289/ehp.793053 · Cited by 34
Metadata
- Authors
- Roy, A. V., Brower, M. E., & Hayden, J. E.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1971-11-01
- Volume
- 17
- Issue
- 11
- Pages
- 1093-1102
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W1899295031
- Cited by
- 210
- References
- 0
- FWCI
- 1.82
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Sodium Thymolphthalein Monophosphate: A New Acid Phosphatase Substrate with Greater Specificity for the Prostatic Enzyme in Serum. https://app.cochat.ai/papers/10.1093/clinchem/17.11.1093
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- Roy, A. V., Brower, M. E., & Hayden, J. E. (1971). Sodium Thymolphthalein Monophosphate: A New Acid Phosphatase Substrate with Greater Specificity for the Prostatic Enzyme in Serum. Clinical Chemistry. https://doi.org/10.1093/clinchem/17.11.1093
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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