Cited by 162Cholesterol and Lipid MetabolismLipid metabolism and disordersLipoproteins and Cardiovascular Health

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Improved estimation of cholesteryl ester transfer/exchange activity in serum or plasma.

Groener, J E, Pelton, R W, Kostner, G M·1986·Clinical Chemistry

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Abstract

This simple, routine assay for measuring cholesteryl ester transfer/exchange activity in human plasma is based on the removal of interfering lipoproteins--very-low-density (VLDL) and low-density lipoproteins (LDL)--by precipitation with polyethylene glycol. High-density lipoproteins (HDL) in the samples do not affect the results. The supernate after precipitation is mixed with [14C]cholesteryl ester-labeled LDL as donor and with HDL as the acceptor for the cholesteryl ester. After incubation for 16 h at 37 degrees C, LDL is separated from HDL by precipitation with dextran sulfate and the radioactivity measured in the supernate, which contains the HDL. The assay is applicable to samples containing as much as 10 mmol of triglycerides per liter. The within-assay CV was 2.7%, the day-to-day CV 6.8%. Results compared well with those by conventional procedures.

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Crossref · 10.1093/clinchem/32.2.283

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Authors: Groener, J. E., Pelton, R. W., & Kostner, G. M.
ORCID: Kostner, G M
Venue: Clinical Chemistry
Publisher: Oxford University Press (OUP)
Published: 1986-02-01
Volume: 32
Issue: 2
Pages: 283-286
ISSN: 0009-9147 (ISSN-L)
1530-8561
DOI: 10.1093/clinchem/32.2.283
OpenAlex: W1917789100
Cited by: 162
References: 0
Citations by year: 2025: 1 · 2021: 1 · 2019: 1 · 2017: 3 · 2016: 3 · 2014: 2 · 2013: 1 · 2012: 2
FWCI: 4.00
Type: journal article
Language: en
Open access: closed
Licence: academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations: Clinical Chemistry (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Cholesterol and Lipid Metabolism
Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipoproteins and Cardiovascular Health
Keywords: cholesteryl ester transfer, serum lipoproteins, HDL cholesterol, LDL cholesterol, VLDL, polyethylene glycol precipitation, dextran sulfate precipitation, assay interference, triglycerides
MeSH terms: Cholesterol Esters, Humans, Isotope Labeling, Lipoproteins, HDL, Lipoproteins, LDL, Cholesterol, LDL, Lipoproteins, VLDL, Mathematics, Polyethylene Glycols, Chemical Precipitation, Scintillation Counting, Ultracentrifugation

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Improved estimation of cholesteryl ester transfer/exchange activity in serum or plasma.

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