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Lipoperoxides in plasma as measured by liquid-chromatographic separation of malondialdehyde-thiobarbituric acid adduct.
Abstract
This assay of plasma lipoperoxides involves hydrolysis in dilute H3PO4 at 100 degrees C; complexation of malondialdehyde (MDA), a hydrolysis product, with thiobarbituric acid (TBA); methanol precipitation of plasma proteins; fractionation of the protein-free extract on a C18 column; and spectrophotometric quantification of the MDA-TBA adduct at 532 nm. The detection limit was 0.15 mumol of MDA per liter of plasma. Run-to-run precision (CV) averaged 8 to 13%. Analytical recovery of MDA after addition of tetraethoxypropane standards to 21 specimens of human or rat plasma averaged 98% (SD 7%). Lipoperoxide concentrations (as MDA) averaged 0.60 (SD 0.13) mumol/L in plasma specimens from 41 healthy persons and 1.4 (SD 0.3) mumol/L in plasma specimens from 12 control rats. Mean lipoperoxide concentrations were 1.5 to 2.3 times as great in plasma sampled from rats one to three days after subcutaneous administration of NiCl2 at dosages (250 to 750 mumol per kilogram body wt) previously shown to induce lipid peroxidation in lung, liver, and kidney.
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Cited by
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Delayed ageing through damage protection by the Arf/p53 pathway
Matheu, A., Maraver, A., Klatt, P., Flores, I., Garcia-Cao, I., Borras, C., Flores, J. M., Viña, J., Blasco, M. A., & Serrano, M. (2007) · Nature · doi:10.1038/nature05949 · Cited by 466
Antioxidant Activities and Oxidative Stress Byproducts in Human Hypertension
Redón, J., Oliva, M. R., Tormos, C., Giner, V., Chaves, J., Iradi, A., & Sáez, G. T. (2003) · Hypertension · doi:10.1161/01.hyp.0000068370.21009.38 · Cited by 420
Grotto, D., Santa Maria, L. D., Boeira, S., Valentini, J., Charão, M. F., Moro, A. M., Nascimento, P. C., Pomblum, V. J., & Garcia, S. C. (2007) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2006.07.030 · Cited by 351
Brain oxidative stress in a triple-transgenic mouse model of Alzheimer disease
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Metadata
- Authors
- Wong, S. H., Knight, J. A., Hopfer, S. M., Zaharia, O., Leach, C. N., & Sunderman, F. W.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1987-02-01
- Volume
- 33
- Issue
- 2
- Pages
- 214-220
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2152555940
- Cited by
- 813
- References
- 0
- FWCI
- 7.05
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Lipoperoxides in plasma as measured by liquid-chromatographic separation of malondialdehyde-thiobarbituric acid adduct. https://app.cochat.ai/papers/10.1093/clinchem/33.2.214
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- Wong, S. H., Knight, J. A., Hopfer, S. M., Zaharia, O., Leach, C. N., & Sunderman, F. W. (1987). Lipoperoxides in plasma as measured by liquid-chromatographic separation of malondialdehyde-thiobarbituric acid adduct. Clinical Chemistry. https://doi.org/10.1093/clinchem/33.2.214
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