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Practical Procedure for Estimation of Corticosterone or Hydrocortisone
Abstract
A simplified fluorometric procedure for corticosterone or hydrocortisone has been described. In spite of certain specificity limitations, it has practical applications for the estimation of these steroids in samples that are small in volume or low in concentration, or in studies that are designed primarily to demonstrate increases or decreases in adrenocortical hormones in plasma or adrenals without requiring absolute specificity. For rat, human, monkey, or guinea pig plasma, 0.5 ml. suffices for an analysis. One rat adrenal or a fraction of a guinea pig adrenal usually contains enough steroid for a single determination. A procedure also has been described which can be applied to rat plasma to correct for interfering fluorescent material.
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Citations
Cited by
Garren, L. D., Ney, R. L., & Davis, W. W. (1965) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.53.6.1443 · Cited by 386
Simple Method of Harvesting Limnoria from Nature
Johnson, F. H., & Ray, D. L. (1962) · Science · doi:10.1126/science.135.3506.795-a · Cited by 276
Intracellular movement of cholesterol in rat adrenal cells. Kinetics and effects of inhibitors
Crivello, J. F., & Jefcoate, C. R. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)70620-6 · Cited by 230
Seelig, S., & Sayers, G. (1973) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(73)90053-2 · Cited by 87
Acute Self-Suppression of Corticosteroidogenesis in Isolated Adrenocortical Cells*
CARSIA, R. V., & MALAMED, S. (1979) · Endocrinology · doi:10.1210/endo-105-4-911 · Cited by 62
Metadata
- Authors
- Silber, R. H., Busch, R. D., & Oslapas, R.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1958-08-01
- Volume
- 4
- Issue
- 4
- Pages
- 278-285
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2165932004
- Cited by
- 544
- References
- 0
- FWCI
- 11.54
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Practical Procedure for Estimation of Corticosterone or Hydrocortisone. https://app.cochat.ai/papers/10.1093/clinchem/4.4.278
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- Silber, R. H., Busch, R. D., & Oslapas, R. (1958). Practical Procedure for Estimation of Corticosterone or Hydrocortisone. Clinical Chemistry. https://doi.org/10.1093/clinchem/4.4.278
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
