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Rapid Semimicro Procedure for Estimating Free and Total Cholesterol
Abstract
A rapid procedure for the determination of both free and total cholesterol in serum is described. The color development is extremely rapid, and once fully developed the color remains stable for several hours. The heat of reaction produced by the combination of concentrated sulfuric acid, phosphoricacid, and glacial acetic acid is apparently responsible for the rapid color development. The stable ferric chloride reagent containingphosphoric acid appears to be the contributing factor to the stability of the color. The results obtained with this procedure compare favorably with those obtained with saponification methods.
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Citations
Cited by
Determination of Lecithin: Cholesterol Acyltransfer in Human Blood Plasma
Stokke, K. T., & Norum, K. R. (1971) · Scandinavian Journal of Clinical and Laboratory Investigation · doi:10.3109/00365517109080184 · Cited by 554
Role of plasma lecithin:cholesterol acyltransferase in the metabolism of high density lipoproteins
Glomset, J. A., Janssen, E. T., Kennedy, R., & Dobbins, J. (1966) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)39245-2 · Cited by 279
Molecular basis of glycoalkaloid induced membrane disruption
Keukens, E. A. J., de Vrije, T., van den Boom, C., de Waard, P., Plasman, H. H., Thiel, F., Chupin, V., Jongen, W. M. F., & de Kruijff, B. (1995) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(95)00186-7 · Cited by 261
Effect of melatonin on the maintenance of cholesterol homeostasis in the rat
Chan, T. Y., & Tang, P. L. (1995) · Endocrine Research · doi:10.1080/07435809509030483 · Cited by 80
Metadata
- Authors
- Courchaine, A. J., Miller, W. H., & Stein, D. B.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1959-12-01
- Volume
- 5
- Issue
- 6
- Pages
- 609-614
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2227540966
- Cited by
- 171
- References
- 0
- FWCI
- 1.94
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Rapid Semimicro Procedure for Estimating Free and Total Cholesterol. https://app.cochat.ai/papers/10.1093/clinchem/5.6.609
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- Courchaine, A. J., Miller, W. H., & Stein, D. B. (1959). Rapid Semimicro Procedure for Estimating Free and Total Cholesterol. Clinical Chemistry. https://doi.org/10.1093/clinchem/5.6.609
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
