The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Downregulation of miR-133 and miR-590 contributes to nicotine-induced atrial remodelling in canines
Abstract
AIMS: The present study was designed to decipher molecular mechanisms underlying nicotine's promoting atrial fibrillation (AF) by inducing atrial structural remodelling. METHODS AND RESULTS: The canine model of AF was successfully established by nicotine administration and rapid pacing. The atrial fibroblasts isolated from healthy dogs were treated with nicotine. The role of microRNAs (miRNAs) on the expression and regulation of transforming growth factor-beta1 (TGF-beta1), TGF-beta receptor type II (TGF-betaRII), and collagen production was evaluated in vivo and in vitro. Administration of nicotine for 30 days increased AF vulnerability by approximately eight- to 15-fold in dogs. Nicotine stimulated remarkable collagen production and atrial fibrosis both in vitro in cultured canine atrial fibroblasts and in vivo in atrial tissues. Nicotine produced significant upregulation of expression of TGF-beta1 and TGF-betaRII at the protein level, and a 60-70% decrease in the levels of miRNAs miR-133 and miR-590. This downregulation of miR-133 and miR-590 partly accounts for the upregulation of TGF-beta1 and TGF-betaRII, because our data established TGF-beta1 and TGF-betaRII as targets for miR-133 and miR-590 repression. Transfection of miR-133 or miR-590 into cultured atrial fibroblasts decreased TGF-beta1 and TGF-betaRII levels and collagen content. These effects were abolished by the antisense oligonucleotides against miR-133 or miR-590. The effects of nicotine were prevented by an alpha7 nicotinic acetylcholine receptor antagonist. CONCLUSION: We conclude that the profibrotic response to nicotine in canine atrium is critically dependent upon downregulation of miR-133 and miR-590.
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Cites
Prediction of Mammalian MicroRNA Targets
Lewis, B. P., Shih, I.-H., Jones-Rhoades, M. W., Bartel, D. P., & Burge, C. B. (2003) · Cell · doi:10.1016/s0092-8674(03)01018-3 · Cited by 5,199
Kannel, W. B., Wolf, P. A., Benjamin, E. J., & Levy, D. (1998) · The American Journal of Cardiology · doi:10.1016/s0002-9149(98)00583-9 · Cited by 2,033 · cited 1×
“Serious, sometimes fatal, cases of atrial fibrillation (AF) have been reported in patients with and without detectable structural and/or history of heart disease from using a nicotine product, particularly when patients have smoked while using nicotine patches.(1,2) In an American Multicenter Study, designed to determ…”
BackgroundBenowitz, N. L., & Gourlay, S. G. (1997) · Journal of the American College of Cardiology · doi:10.1016/s0735-1097(97)00079-x · Cited by 529
Cited by
Epidemiology of Atrial Fibrillation in the 21st Century: Novel Methods and New Insights
Kornej, J., Börschel, C. S., Benjamin, E. J., & Schnabel, R. B. (2020) · Circulation Research · doi:10.1161/circresaha.120.316340 · Cited by 1,516
Atrial Fibrillation: Epidemiology, Pathophysiology, and Clinical Outcomes
Staerk, L., Sherer, J. A., Ko, D., Benjamin, E. J., & Helm, R. H. (2017) · Circulation Research · doi:10.1161/circresaha.117.309732 · Cited by 1,201
Atrial Remodeling and Atrial Fibrillation
Nattel, S., & Harada, M. (2014) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2014.02.555 · Cited by 736
Prevention of Atrial Fibrillation: Report From a National Heart, Lung, and Blood Institute Workshop
Benjamin, E. J., Chen, P.-S., Bild, D. E., Mascette, A. M., Albert, C. M., Alonso, A., Calkins, H., Connolly, S. J., Curtis, A. B., Darbar, D., et al. (2009) · Circulation · doi:10.1161/circulationaha.108.825380 · Cited by 470
MicroRNA-328 Contributes to Adverse Electrical Remodeling in Atrial Fibrillation
Lu, Y., Zhang, Y., Wang, N., Pan, Z., Gao, X., Zhang, F., Zhang, Y., Shan, H., Luo, X., Bai, Y., et al. (2010) · Circulation · doi:10.1161/circulationaha.110.958967 · Cited by 451
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Metadata
- Authors
- Shan, H., Zhang, Y., Lu, Y., Zhang, Y., Pan, Z., Cai, B., Wang, N., Li, X., Feng, T., Hong, Y., & Yang, B.
- Venue
- Cardiovascular Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-04-27
- Volume
- 83
- Issue
- 3
- Pages
- 465-472
- ISSN
- 0008-6363 (ISSN-L)
- 1755-3245
- OpenAlex
- W2131901732
- Cited by
- 349
- References
- 3
- FWCI
- 11.07
- Type
- journal article
- Language
- en
- Open access
- closed
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- Shan, H., Zhang, Y., Lu, Y., Zhang, Y., Pan, Z., Cai, B., Wang, N., Li, X., Feng, T., Hong, Y., & Yang, B. (2009). Downregulation of miR-133 and miR-590 contributes to nicotine-induced atrial remodelling in canines. Cardiovascular Research. https://doi.org/10.1093/cvr/cvp130
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