Isolation and Expression Profiling of Genes Upregulated in Bone Marrow-Derived Mononuclear Cells of Rheumatoid Arthritis Patients
Open accessAbstract
We have comprehensively identified the genes whose expressions are augmented in bone marrow-derived mononuclear cells (BMMC) from patients with Rheumatoid Arthritis (RA) as compared with BMMCs from Osteoarthritis (OA) patients, and named them AURA after augmented in RA. Both stepwise subtractive hybridization and microarray analyses were used to identify AURA genes, which were confirmed by northern blot analysis and/or reverse transcription polymerase chain reaction (RT-PCR). We also assessed their expression levels in individual patients by quantitative real-time RT-PCR. Of 103 AURA genes we have identified, the mRNA levels of the following 10 genes, which are somehow related to immune responses, were increased in many of the RA patients: AREG (=AURA9), FK506-binding protein 5 (FKBP5 = AURA45), C-type lectin superfamily member 9 (CLECSF9 = AURA24), tyrosylprotein sulfotransferase 1 (TPST1 = AURA52), lymphocyte G0/G1 switch gene (G0S2 = AURA8), chemokine receptor 4 (CXCR4 = AURA86), nuclear factor-kappa B (NF-kappaB = AURA25) and two genes of unknown function (FLJ11106 = AURA1, BC022398 = AURA2 and XM_058513 = AURA17). Since AREG was most significantly increased in many of the RA patients, we subjected it to further analysis and found that AREG-epidermal growth factor receptor signaling is highly activated in synovial cells isolated from RA patients, but not in OA synoviocytes. We propose that the expression profiling of these AURA genes may improve our understanding of the pathogenesis of RA.
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Clec4e mRNA is upregulated in patients with rheumatoid arthritis.
“Interestingly, Clec4e mRNA is upregulated in patients with rheumatoid arthritis73,74.”
From Sterile inflammation: sensing and reacting to damage (2010) · cites this paper as [74]
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Sterile inflammation: sensing and reacting to damage
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“Another study found FKBP51 and NF-kB up-regulated in bone marrow-derived mononuclear cells from patients with rheumatoid arthritis [83].”
- Authors
- Nakamura, N., Shimaoka, Y., Tougan, T., Onda, H., Okuzaki, D., Zhao, H., Fujimori, A., Yabuta, N., Nagamori, I., Tanigawa, A., Sato, J., Oda, T., Hayashida, K., Suzuki, R., Yukioka, M., Nojima, H., & Ochi, T.
- Affiliations
- Yukioka Hospital (JP)
- Research Institute for Microbial Diseases, The University of Osaka (JP)
- Osaka Minami Medical Center (JP)
- National Sagamihara Hospital (JP)
- Corresponding author
- Nojima, H.
- Venue
- DNA Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-01-01
- Volume
- 13
- Issue
- 4
- Pages
- 169-183
- ISSN
- 1340-2838 (ISSN-L)
- OpenAlex
- W2146752492
- Cited by
- 94
- References
- 58
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2023: 3 · 2022: 7 · 2021: 3 · 2020: 4 · 2019: 3 · 2018: 4 · 2017: 6 · 2016: 8 · 2015: 4 · 2014: 5 · 2013: 8 · 2012: 9
- FWCI
- 2.84
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- DNA Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science
- Japan Science and Technology Agency
- Topics
- Health Sciences › Medicine › Rheumatology › Rheumatoid Arthritis Research and Therapies
- Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
- Health Sciences › Medicine › Hematology › Autoimmune and Inflammatory Disorders Research
- Keywords
- rheumatoid arthritis, osteoarthritis, bone marrow mononuclear cells, gene expression profiling, amphiregulin, epidermal growth factor receptor signaling, synoviocytes, immune response genes, NF-κB, CXCR4, FKBP5, G0S2, pathogenesis
- MeSH terms
- Arthritis, Rheumatoid, Bone Marrow Cells, Cells, Cultured, Genes, Glycoproteins, Humans, Leukocytes, Leukocytes, Mononuclear, Models, Biological, Osteoarthritis, Synovial Fluid, Up-Regulation, DNA, Complementary, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Intercellular Signaling Peptides and Proteins, Cell Proliferation, EGF Family of Proteins, Amphiregulin
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